Detection probe for leptocylindrus danicus
A technology for detecting probes and Cylindrophyllum, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of large fluctuations in detection results, difficult operation, and small number of capture probes, so as to facilitate the search and implementation , good probe applicability and high stability
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[0030] Example: Qualitative and quantitative detection of Cylindrocystis danishensis by using S1 enzyme protection analysis for rRNA and sandwich hybridization technology.
[0031] In this example, the combination of S1 enzyme protection analysis technology and sandwich hybridization technology is used to realize the qualitative and quantitative detection of marine red tide organism Leptocylindrus danicus. The steps are as follows:
[0032] 1.1 The search for the specific region of Cylindrocystis danishensis rRNA and the design and synthesis of S1 enzyme protection analysis probe
[0033] By determining the 18S rRNA sequence of the ribosomal small subunit of Cylindrophyllum danishensis and performing multiple sequence comparisons with the rRNA sequences of other algae, it was found that the 30-100 region of the small subunit rRNA was different from other algae, and the sequence of this region was determined to be a characteristic sequence ; Design and synthesize the S1 enzyme ...
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