This invention belongs to the field of
biomedical technology, specifically disclosing the application of
mancozeb in the preparation of ERK inhibitors. Through
zebrafish embryo models and HEK293
T cell experiments, this invention demonstrates that
mancozeb can specifically inhibit the MAPK / ERK pathway as an ERK inhibitor, and that at concentrations of 2μM to 8μM, it can induce a three-dimensional spiral notochord malformation in
zebrafish embryos similar to that caused by 100μM U0126 treatment, providing a new approach for the preparation of MAPK / ERK pathway inhibitors.