Method for fast breeding water caltrop seedlings
A technology for seedlings and seedlings of Smilax, which is applied in the field of rapid propagation of Smilax seedlings, and achieves the effect of simple source of materials and low cost
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[0037] A method for rapid propagation of Smilax seedlings, which comprises the following steps:
[0038] (A) collect healthy and robust Paleopsis seedlings from natural water bodies or in greenhouses in winter or spring;
[0039] (B) Rinse with tap water for 1-2 hours in the laboratory to remove the pollutants on the surface of the weed grass. After the weed grass seedlings are rinsed, remove the leaves and roots, cut the thick stems near the top, and rinse them with distilled water for 2-3 times. Wash the weed stems thoroughly;
[0040] (C) Sterilization and bud induction: tools such as plates, tweezers, and culture media that need to be used during the experiment must be sterilized by 121°C 0.1MPa high-pressure steam, and the experimental tools should be placed in a biological safety cabinet or Sterilize in the sterile operating table for 30 minutes, and then use sterilizing agents to sterilize under aseptic conditions. The sterilizing agents are PPM-Plant preserve Mixture,...
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