Microorganism water treatment agent in culturing water and preparation technique thereof
A technology for cultivating water and microorganisms, applied in the field of animal breeding, can solve problems such as weak stress resistance and poor stability, and achieve the effects of improving water quality, reducing usage, and improving breeding benefits
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[0025] (1) Bacteria are selected for the compound microbial preparation. The compound microbial preparation is composed of Klebsiella AN-4, Bacillus subtilis (code name CICC 10074), Rhodospirillum (code name ACCC02976) and plant Lactobacillus (Lactobacillus plantarum, ACCC 10643) of. Klebsiella AN-4 was screened and provided by our laboratory, and the NCBI accession number is EU888474. Bacillus subtilis, Rhodospirillum and Lactobacillus plantarum were purchased from Yangzhou Gaoyou Lvke Biological Co., Ltd.
[0026] (2) Klebsiella AN-4 and Bacillus subtilis were respectively inserted into the triangular flask (medium: peptone 10g / mL, beef extract 5g / mL, NaCl 5g / mL, inoculation ratio 1%), shaking culture for 24 h . Rhodospirillum was inserted into the Kirschner flask (medium: NH 4 Cl 1g, MgCl 2 0.2g, yeast extract 0.1g, K 2 HPO 4 0.5g, NaCl 2g, H 2 O 900 mL, sterile 0.1 g / mL NaHCO 3 50mL, 50mL sterilizing alcohol. Inoculation ratio 1%), cultured in a biochemical inc...
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