Microorganism water treatment agent in culturing water and preparation technique thereof

A technology for cultivating water and microorganisms, applied in the field of animal breeding, can solve problems such as weak stress resistance and poor stability, and achieve the effects of improving water quality, reducing usage, and improving breeding benefits

Inactive Publication Date: 2010-01-13
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product is low in cost, green and environmentally friendly, and overcomes the problems of poor stability and poor stress resistance of current water purifiers. It can be used in large-scale industrial production and provides a new type of microbial preparation for the purification of aquaculture water.

Method used

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  • Microorganism water treatment agent in culturing water and preparation technique thereof
  • Microorganism water treatment agent in culturing water and preparation technique thereof
  • Microorganism water treatment agent in culturing water and preparation technique thereof

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Experimental program
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Embodiment Construction

[0025] (1) Bacteria are selected for the compound microbial preparation. The compound microbial preparation is composed of Klebsiella AN-4, Bacillus subtilis (code name CICC 10074), Rhodospirillum (code name ACCC02976) and plant Lactobacillus (Lactobacillus plantarum, ACCC 10643) of. Klebsiella AN-4 was screened and provided by our laboratory, and the NCBI accession number is EU888474. Bacillus subtilis, Rhodospirillum and Lactobacillus plantarum were purchased from Yangzhou Gaoyou Lvke Biological Co., Ltd.

[0026] (2) Klebsiella AN-4 and Bacillus subtilis were respectively inserted into the triangular flask (medium: peptone 10g / mL, beef extract 5g / mL, NaCl 5g / mL, inoculation ratio 1%), shaking culture for 24 h . Rhodospirillum was inserted into the Kirschner flask (medium: NH 4 Cl 1g, MgCl 2 0.2g, yeast extract 0.1g, K 2 HPO 4 0.5g, NaCl 2g, H 2 O 900 mL, sterile 0.1 g / mL NaHCO 3 50mL, 50mL sterilizing alcohol. Inoculation ratio 1%), cultured in a biochemical inc...

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Abstract

The invention relates to a microorganism water treatment agent in culturing water and a preparation technique thereof, belonging to the technical field of animal farming. The microorganism water treatment agent comprises (Klebsiella) AN-4, bacillus subtilis, Rhodospirillum, and Lactobacillus plantarum. Bean cake powder and molasses are used as raw materials and deeply fermented to produce water treatment agent which has lots of living bacterial and can effectively purify water quality. The product has low cost and environmental protection, overcomes problems that the current water quality purifying agent has poor stability and weak stress resistance and the like, can be used for large-scale industrial production and provides a new microorganism preparation for aquaculture water body.

Description

technical field [0001] The invention relates to a microbial water purifier for aquaculture water and a preparation process thereof, belonging to the technical field of animal aquaculture. Background technique [0002] Studies have shown that in today's aquaculture process, due to the low bait utilization rate, excessive discharge of residual bait, and excessive discharge of farmed animal feces, eutrophication of water bodies, C, N, and P enrichment pollution of sediments and sediment oxidation are caused. The reduction potential is lowered. Increased eutrophication of water bodies will lead to excessive ammonia nitrogen and nitrite, which will have a toxic effect on aquatic animals. The discharge of a large amount of aquaculture wastewater has caused a huge impact on the surrounding environment. The water environment has deteriorated, red tides have occurred frequently, and ecological balance and biodiversity have also been destroyed. The decline in water quality in aquacu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01K63/04C12N1/20C12R1/22C12R1/125C12R1/25C12R1/01
Inventor 刘文斌高宇方星星桂丹朱浩
Owner NANJING AGRICULTURAL UNIVERSITY
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