131 results about "Klebsiella species" patented technology

Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans.

Disclosed is a method for detecting the species of a bacterium in a simple manner. Specifically disclosed is a method for detecting the species of a bacterium, which can detect at least one bacterial species selected from Staphylococcus species, Streptococcus species, Klebsiella species, Escherichia species, Mycobacterium species, Legionella species, Vibrio species, Bacillus species, Neisseria species, Campylobacter species, Chlamydia species, Chlamydophila species, Mycoplasma species, Listeria species, Salmonella species and Yersinia species, and which comprises the steps of: a) contacting a sample suspected to contain a nucleic acid derived from the bacterium species to be detected with a nucleic acid molecule capable of hybridizing with at least a part of a DnaJ gene derived from the bacterial species; and b) detecting the presence or absence of the hybridization between the nucleic acid molecule and the nucleic acid contained in the sample.

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing microorganisms, such as, Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Escherichia, Salmonella, Bacillus, Streptomyces and Pseudomonas, containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions. Specifically, Citrobacter and, Klebsiella provide the source of exogenous genes for such active dehydratase enzyme.

The invention relates an air cleaner, solving the problem that the current air cleaners isn't disclosed the date of removing pneumococcus klebsiella and staphylococcus epidermidis and the date of effective degerming life, and the cleaning effect of current air cleaners is not good. The invention adopts the technical project that the silver particles below 100 nanometer are fixed on the surface of carrier to solve the problem.

The present invention describes an L-glutamic acid-producing bacterium which belongs to the genus Pantoea, Enterobacter, Klebsiella or Erwinia, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

An organic acid is produced by allowing a bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an organic acid and has been modified so that the phosphoenolpyruvate carboxykinase activity is enhanced, which is selected from Enterobacter, Pantoea, Erwinia, Klebsiella and Raoultella bacteria, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.

The invention discloses application of elsholtzia volatile oil in anti-bacteria and antibiotic resistant bacteria. The elsholtzia volatile oil is extracted by steam distillation. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of staphylococcus, escherichia coli, candida, pseudomonas, enterobacter, streptococcus and the like and fungi. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of drug-resistant staphylococcus, drug-resistant escherichia coli, drug-resistant enterobacter, drug-resistant klebsiella, drug-resistant pseudomonas, drug-resistant acinetobacter, drug-resistant streptococcus, and drug-resistant enterococcus and the like. The elsholtzia volatile oil can be used in industries offood, medicine, health care products, cosmetics and the like and can be used for preparing various forms of products with antibacterial and antibiotic resistant bacteria functions, and belongs to thenew application field of the elsholtzia volatile oil.

The invention relates to denitrifying phosphorus accumulation organism (DPAO) with a function of efficiently removing nitrogen and phosphorus and application of the DPAO, and belongs to the field of environmental microbiology. The bacterial strain is named Klebsiella sp. N14, belongs to Klebsiella species, and is preserved in China general microbiological culture center (CGMCC), the preservation number of the bacterial strain is CGMCC No. 10290. The bacterial strain is a Gram negative bacteria without spore and has thick capsule in a thick and short rod shape; the bacterial colony is milky white, the center of the bacteria is protruded, the edge is regular, and the surface is wet, transparent and glossy. The bacterial strain has a function of synchronously removing the nitrogen and the phosphorus, and can be applied to sewage biological sewage treatment process; after the bacterial strain is aerobically cultivated in nitrogen and phosphorus containing sewage for 24 hours, the phosphorous removal rate is 81.79%, and the nitrogen removal rate is 85.94%. The denitrifying final product of the bacteria strain is identified to be N2 with the full denitrifying capability; when the bacterial strain is used for treating the nitrogen and phosphorus containing sewage, inorganic nitrogen, such as nitrate nitrogen and nitrite nitrogen, in a water body is directly reduced to be harmless nitrogen gas to be discharged out of the water body; the effect is remarkable, the cost is low, and no secondary pollution exists.

The invention discloses microbes capable of degrading phenol and cyanogen in coking waste water and a method for treating coking waste water by using the same, which relate to microbes for degrading phenol and cyanogen in coking waste water and a method for treating waste water by using the same. The invention solves the problem that micromolecule harmful substances in the coking waste water treatment process, such as volatile phenol and cyanides, are difficult to remove. The microbes capable of degrading the phenol and the cyanogen in the coking waste water comprise bacillus subtilis BHF3-4, bacillus JhqK9-1 and acid-producing klebsiella JhqM8. The method for treating the coking waste water comprises the following steps: 1. inoculating the microbes capable of degrading the phenol and the cyanogen in the coking waste water to a membrane biological reactor (MBR); 2. and treating the coking waste water. When the method is used for treating the coking waste water, the removal rate of the volatile phenol in the coking waste water can reach 99.6-99.9%, and the removal rate of cyanides can reach 99.5-99.7%, thus, the outlet water can reach the Chinese first-class emission standard.

The invention discloses establishment and application of a Klebsiella arcA gene-deleted strain. In the invention, the key gene arcA inhibiting TCA cycle of 1,3-propylene glycol producing Klebsiella is knocked out by a homologous recombination method, the TCA cycle activity of cells in a micro-aerobic condition is increased, the reducing power regeneration is enhanced, and the yield of 1,3-propylene glycol is increased. The yield of 1,3-propylene glycol synthesized by fermenting glycerin with knockout bacteria K.pneumoniae (delta)arcA under micro-aerobic conditions is increased by 12.57% to 17.64g/L, indicating that the yield of 1,3-propylene glycol can be effectively increased by knocking out the arcA gene participating in TCA cycle transcription regulation.

The invention provides denitrifying strain Klebsiella sp. KSND-3 with simultaneous aerobic nitrification and denitrification performances and preparation and application thereof. The present inventionalso provides a Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage. Because of fast growth rate, high cell yield and low dissolved oxygen concentration demand of the Klebsiella strain, the Klebsiella strain can be used for simultaneous nitrification and denitrification, and the denitrification process is more concise, economical and effective.

The invention relates to a chlorimuron-ethyl pesticide residue degrading bacterium which is named klebsiella 2N3 (Klebsiella jilinsis H.Zhang 2N3) with the storage No. of CCTCC NO: M 209248 in China Center for Type Culture Collection (CCTCC), a soil bioremediation agent prepared from the chlorimuron-ethyl pesticide residue degrading bacterium, and application thereof. The chlorimuron-ethyl pesticide residue degrading bacterium is a bacterial strain which has strong degradation capacity to chlorimuron-ethyl and is obtained by carrying out enrichment culture and separation on sludge at a drainage outlet of an insecticide factory for producing chlorimuron-ethyl, the bacterial strain grows by using the chlorimuron-ethyl as the unique nitrogen source, and the activity of the bacterium in a liquid nutrient medium is increased by the induction of the chlorimuron-ethyl so as to improve the degradation capacity to the chlorimuron-ethyl. The preparation method for the bacterium is simple, low in cost and convenient to use, and can be spread in the soil, and the degradation effect in the soil can reach 82.6 percent; therefore, the chlorimuron-ethyl pesticide residue degrading bacterium has good application prospect.

The present invention provides a microorganism for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising a) at least one gene encoding a dehydratase activity; b) at least one gene encoding a glycerol-3-phosphatase; and c) at least one gene encoding protein X. The protein X may be derived from a Klebsiella or Citrobacter gene cluster. The recombinant microorganism may further comprise d) at least one gene encoding a protein having at least 50% similarity to a protein selected from the group consisting of protein 1 (SEQ ID NO:60 or SEQ ID NO:61), of protein 2 (SEQ ID NO:62 or SEQ ID NO:63) and of protein 3 (SEQ ID NO:64 or SEQ ID NO:65) from Klebsiella or Citrobacter.

The invention provides klebsiella for strengthening the expression of a citT gene and an application of the klebsiella in production of 1,3-propylene glycol. The klebsiella provided by the invention is specifically obtained by strengthening the expression of the citT gene in the klebsiella. By applying klebsiella provided by the invention to fermentation production of 1,3-propylene glycol, the yield of 1,3-propylene glycol can be increased.

The invention discloses a multiplex quantitative PCR (Polymerase Chain Reaction) primer, a probe, a kit and a detection method for corynebacterium bacteria and Klebsiella corynebacterium. Aiming at corynebacterium bacterium 23S rRNA genes and Klebsiella corynebacterium 16S rRNA gene sequences, specific primers and probes are designed, whether the corynebacterium bacteria and Klebsiella corynebacterium exist in a biological sample can be identified by utilizing multiplex quantitative PCR. The primers and probes have an important guiding role in clinical targeted selective use of antibiotics. Due to reasonable usage of the antibiotics, the course of granulomatous mastitis can be effectively shortened, the condition that breast tissues are removed during the operation is avoided, physical and psychological trauma of a patient can be greatly alleviated, and an etiological basis is provided for clinical diagnosis and treatment of the granulomatous mastitis.

The invention discloses a preparing method of restructuring strain in biological technical domain, which comprises the following steps: adopting PCR technique; cloning dhaT gene from pneumonic klebsiella DSM 2026; constructing bacillus coli expressing carrier pET-dhaT with dhaT gene; transferring into bacillus coli DH5 alpha; proceeding plasmid reproduction; extracting recombinant plasmid; transferring to bacillus coli BL21(DE3); getting recombination gene engineering strain E. coli-pET-dhaT to express 1, 3-propanediol oxidoreductase (PDOR). This strain can get stable PDOR under the condition of non-antibiotic selective pressure.

The invention provides a primer compound for identifying intestinal microecological state and an application thereof. The primer compound is a specific primer designed for intestinal bacterium 16S rRNA; the specific primer includes random basic groups; the quantity of random basic groups is 3-5; the intestinal bacterium includes any one of or combination of at least two of symbiotic clostridia, bifidobacterium adolescentis, fusobacterium nucleatum, peptostreptococcus anaerobius or klebsiella. A kit researched and developed on the basis of the primer compound provided by the invention has a specificity for detecting intestinal microecological imbalance reaching up to 92.5% and a sensitivity reaching up to 80.43%.

The present invention is related with the Microbiology field and particularly with a composition and a method for early detection, identification, differentiation and count of microscopic organisms, concretely Gram-negative microorganisms. The composition described in the invention consist on a mixture of substances of protein origin with a total nitrogen content from 9 to 20% and in relationship between 2:1 to 24:1, concerning to the content of inhibitors of the Gram-positive organisms. It contains a mixture of organic and inorganic substances that facilitate the differentiation of the Gram-negative organisms, being this mixture in a relationship from 0.5:1 to 2:1 concerning to the mixture of substances of protein origin. The referred composition allows the detection and differentiated count of E. coli and other coliform organisms due to the blue-greenish color of the colonies of these microorganisms on the orange bottom of the medium; Salmonella not typhi for the red color of the centers of the colonies on rosy bottom of the medium; Salmonella typhi and Proteus for the transparency of the colonies; Citrobacter and Klebsiella for the violet color of the colonies on the pink to orange bottom of the medium and Pseudomonas aeruginosa for the orange color with darker center of the colony, taking greenish pigmentation after 24 hours and producing greenish fluorescence under low ultraviolet light.

Embodiments of the invention provide a method of detecting one or more strains of Klebsiella pneumoniae. The method may include forming a plurality of mixtures for nucleic amplification. The method can include amplification of specific sequences within the K. pneumonia genome that can provide definitive information to distinguish between one or more types or strains of K. pneumonia.