Multiplex quantitative PCR (Polymerase Chain Reaction) primer, probe, kit and detection method for corynebacterium bacteria and Klebsiella corynebacterium

Abstract

The invention discloses a multiplex quantitative PCR (Polymerase Chain Reaction) primer, a probe, a kit and a detection method for corynebacterium bacteria and Klebsiella corynebacterium. Aiming at corynebacterium bacterium 23S rRNA genes and Klebsiella corynebacterium 16S rRNA gene sequences, specific primers and probes are designed, whether the corynebacterium bacteria and Klebsiella corynebacterium exist in a biological sample can be identified by utilizing multiplex quantitative PCR. The primers and probes have an important guiding role in clinical targeted selective use of antibiotics. Due to reasonable usage of the antibiotics, the course of granulomatous mastitis can be effectively shortened, the condition that breast tissues are removed during the operation is avoided, physical and psychological trauma of a patient can be greatly alleviated, and an etiological basis is provided for clinical diagnosis and treatment of the granulomatous mastitis.

Description

Technical field: [0001] The invention belongs to the fields of molecular biology and medicine, and in particular relates to a multiple fluorescent quantitative PCR primer, probe, kit and detection method for bacteria belonging to the genus Corynebacterium and Corynebacterium krusei. Background technique: [0002] In recent years, in clinical practice in hospitals, it has been found that the incidence of non-lactating mastitis has gradually increased, even exceeding that of lactating mastitis. Granulomatous mastitis is an important non-lactating mammary gland inflammatory disease. Its clinical manifestations are mammary gland mass, abscess sinus tract or ulcer. The etiology of granulomatous mastitis is complex, involving local trauma, autoimmunity, bacterial infection and other factors. In recent years, more and more domestic and foreign studies have shown that Corynebacterium infection plays an important role in the development of granulomatous mastitis lesions. Recently, ...

AI Technical Summary

Problems solved by technology

Since Corynebacterium and Corynebacterium krusei are hard-to-cultivate bacteria, there are problems such as long time-consuming, poor accuracy, and low positive rate, which cannot meet the needs of clinical diagnosis and treatment.

Images