197results about How to "Reduce economic spending" patented technology

The invention provides a humanized PD-L1 tumor cell line MC-38-hPD-L1, a builtanimal tumor model with the same and a method for constructing the humanized PD-L1 tumor cell line. The method particularly includes knocking out animal-origin PD-L1 by the aid of CRISPR-CAS9; carrying out amplification and cultivation to obtain knocked-out cell banks; extracting DNA (deoxyribonucleic acid) and carrying out PCR (polymerase chain reaction) amplification; recycling and cloning amplification products; carrying out over-expression on human-origin PD-L1 in MC-38 cell lines of mPD-L1 KO by the aid of lentivirus systems; packaging lentivirus and screening Puromycin to obtain the humanized MC-38 cell line of PD-L1. The humanized PD-L1 tumor cell line, the animal tumor model and the method have the advantages that as shown by results, high killing efficiency and multiplication capacity are obviously presented by tumor infiltration CD8 T lymphocytes after antibody treatment is carried out, tumor infiltration Treg cells can be obviously inhibited after antibody treatment is carried out, and accordingly the method is proved to be effective and feasible from the aspect of molecular mechanisms.

The invention relates to a cutter head structure of a detachable electric shaver, comprising a plurality of cutter blade assemblies capable of rotatably cutting, and the cutter blade assemblies are injoggled connection with a central transmission gearwheel on a main support by respective transmission unit. The cutter head structure is characterized in that the main support is sleeved on the lowercover of the main support, whereas the lower cover of the main support is connected with a cutter blade connecting seat by spherical coordination; and an elastic element is supported between the mainsupport and the cutter blade connecting seat. The cutter head structure has big rotational degree of freedom, simple and practical structure and easy manufacture.

The invention provides a PSCA (prostate stem cell antigen) and PD-L1 targeted CAR based on OCTS (one CAR with two ScFvs)-CAR (chimeric antigen receptor), an encoding gene, an OCTS-CAR-T recombinant expression vector as well as a construction method and an application of the OCTS-CAR-T recombinant expression vector. The PSCA and PD-L1 targeted CAR comprises a CD8 leader membrane receptor signal peptide, a double-antigen binding region, a CD8 Hinge chimeric receptor linker, a CD8 Transmembrane chimeric receptor transmembrane region, a CD28 chimeric receptor co-stimulator, an OX40 chimeric receptor co-stimulator and a TCR chimeric receptor T-cell activation domain which are connected in series sequentially, wherein the double-antigen binding region comprises heavy chains VH and light chains VL of PSCA and PDL1 single-chain antibodies, Inner-Linker in antibodies and Inter-Linker among the single-chain antibodies, which are in series connection or turn connection. Besides, the invention provides the encoding gene of the PSCA and PD-L1 targeted CAR, the recombinant expression vector as well as the construction method and the application of the recombinant expression vector.

The invention provides an OCTS-CAR technique-based OCTS-CAR double-targeting chimeric antigen receptor, a coding gene and a recombinant expression vector and establishment and application of the OCTS-CAR double-targeting chimeric antigen receptor, the coding gene and the recombinant expression vector. The OCTS-CAR double-targeting chimeric antigen receptor includes a CD8 leader membrane receptor signal peptide, a double-antigen binding region, a CD8 Hinge chimeric receptor gemel, a CD8 transmembrane chimeric receptor transmembrane region, a CD28 chimeric receptor co-stimulatory factor, a CD134 chimeric receptor co-stimulatory factor and a TCR chimeric receptor T cell activating domain which are connected sequentially and in series, wherein the double-antigen binding region comprises heavy-chain VH and light-chain VL, connected in a certain mode, of two single-chain antibodies, an antibody Inner-Linker and an Inter-Linker between single-chain antibodies, and the two single-chain antibodies are formed by combining any two of a BCMA single-chain antibody, a CD319 single-chain antibody, a CD38 single-chain antibody, a PDL1 single-chain antibody and a CD123 single-chain antibody; in addition, the invention further provides a gene encoding the OCTS-CAR double-targeting chimeric antigen receptor, the recombinant expression vector and an establishment method and application of the gene encoding the OCTS-CAR double-targeting chimeric antigen receptor and the recombinant expression vector.

The invention relates to a sieve mesh with adjustable sieve pore gaps. An edge frame is provided with a group of movable frames moving along an X shaft and a group of movable frames moving along a Y shaft; an upper beam which is uniformly distributed and made by each movable frame moving along the X shaft is respectively positioned between adjacent longitudinal rods, is parallel to the longitudinal rods and positioned in the same plane, and a lower beam of the movable frame slides in a guiding manner by a guide sleeve fixedly arranged on the edge frame; and an upper beam which is uniformly distributed and made by each movable frame moving along the Y shaft is respectively positioned between adjacent horizontal rods, is parallel to the horizontal rods and positioned in the same plane, and alower beam of the movable frame slides in a guiding manner by a guide sleeve fixedly arranged on the edge frame. The sieve mesh has reasonable design, convenient use, high sieving precision and highefficiency, is economical and practical, and can adjust the size of the sieve pore continuously; and the sieve mesh can adjust the size of the sieve pore in a certain range, is not limited by the specification when in use and can realize the multiuse effect with one mesh.

The invention discloses a method for automatically shutting down a TV set and a TV set, which control the TV set to automatically enter a sleep detection process within a preset sleep time period according to a user's sleeping habits, and use information sharing between smart home devices to detect Whether the TV and other smart home devices in the same local area network have received active operation instructions from the user in the recent period of time, or whether the user has entered a sleep state is further combined with the wearable electronic products worn by the user. This can improve the accuracy of sleep detection results, avoid the accidental interruption of TV programs watched by the user due to the wrong shutdown of the TV, while ensuring that the user can watch TV programs normally, by controlling the TV after the user falls asleep The automatic shutdown can effectively reduce the waste of power resources, and make the user's sleep quality not affected by the sound and light emitted by the TV.

The present invention relates to one composition for preventing and treating cardiac and cerebral vascular diseases and the composition may be used as medicine, health food or health food additive. The composition consists of red yeast and haw or haw leaf, and may contain gingko leaf and lotus leaf. The composition is used in preventing and treating hypertension, hyperlipemia, etc causing cardiac and cerebral vascular diseases, and has determined curative effect and no toxic side effect.

The invention discloses a cleaning technology suitable for processing an oil recovery waste water polytetrafluoroethylene film, and belongs to the technical field of film cleaning. The cleaning technology comprises the following steps: (1) cleaning the film for 0.5-1 h with water; (2) adding an oxidant or a surfactant in sodium hydroxide solution as alkali cleaning solution to clean the film for 0.5-1 h; soaking the film for 0.5-5 h; soaking the film in water for 0.5-1 h; (3) cleaning the film with an organic solvent for 0.3-0.5h; soaking the film for 0.5-1 h; (4) cleaning the film with acidic cleaning solution for 0.5-1 h; soaking the film for 0.5-5 h; soaking the film in water for 0.5-1 h; ending the whole cleaning process. The technology is high in cleaning efficiency of the film, can effectively clean out organic matters, inorganic matters and microbes on the surface of the film and in the film openings; clear water flux restoring rate can reach above 98%; the technology is high in detersive efficiency and pertinence, economical, and high in energy conservation.

The invention discloses a cleaning and drying device for a small metal part. The cleaning and drying device comprises a cleaning box; an orifice plate is horizontally and fixedly arranged at the center in the cleaning box; the top surface of the orifice plate is fixedly attached with a filter screen; a submerged pump is arranged at the lower end of the orifice plate; a water outlet pipe is fixedlyconnected with the water oultet of the the submerged pump; the other end of the water outlet pipe penetrates through the side wall of the cleaning boxand protrudes out of the cleaning box; a fixing plate is fixed at one side of the cleaning box; a hydraulic station is fixedly arranged on the fixing plate, and the upper end of the cleaning box is provided with a washing tank. The device can achieve the purpose of cleaning and drying the small metal part effectively through the arrangement of the cleaning box and the drying box, and the labor is replaced effectively; the cost is low, the efficiency is high; the washing water and the sawdust in the cleaning box and the drying box can be recycled for a long time through the arrangement of the filter screen and a hot-air blower; the sufficient use rate of the washing water is improved effectively and the economical expense is saved.

The invention discloses an OCTS (One CAR (Chimeric Antigen Receptor) with two ScFvs (Single-chain variable Fragments)) technique based CAR-T (Chimeric Antigen Receptor-T cell immunotherapy) therapeutic vector for glioblastoma. The OCTS technique based CAR-T therapeutic vector comprises a lentiviral skeleton plasmid, a human EF1 [alpha] promoter (SEQ ID NO. 14), an OCTS chimeric receptor structural domain and a PDL1 single-chain antibody, wherein the OCTS chimeric receptor structural domain comprises a CD8 leader chimeric receptor signal peptide (SEQ ID NO. 15), a PDL1 single-chain antibody light chain VL (SEQ ID NO. 16), a PDL1 single-chain antibody heavy chain VH (SEQ ID NO. 17), an EGFRvIII single-chain antibody light chain VL (SEQ ID NO. 18), an EGFRvIII single-chain antibody heavy chain VH (SEQ ID NO. 19), an antibody Inner-Linker (SEQ ID NO. 20), a single-chain antibody Inter-Linker (SEQ ID NO. 21), a CD8 Hinge chimeric receptor linker (SEQ ID NO. 22), a CD8 Transmembrane chimeric receptor transmembrane domain (SEQ ID NO. 23), a TCR (T Cell Receptor) chimeric receptor T cell activation domain (SEQ ID NO. 26) and a chimeric receptor co-stimulator domain. In addition, the invention also discloses a construction method of the vector and application of the vector to the preparation of a medicine for treating the glioblastoma.

The invention relates to a method for detecting leakage of insulating oil in an on-load tapping switch of a transformer by using SF6 gas, which comprises the following steps: firstly, calculating volume of the SF6 gas in the insulating oil added to the on-load tapping switch; adding the SF6 gas to the insulating oil for the on-load tapping switch according to a calculated value; selecting an oil sample of transformer body oil; and using a gas chromatograph to carry out chromatographic analysis for the selected oil sample of the transformer body oil. If the SF6 gas is detected out, which proves that the on-load tapping switch leaks the insulating oil; and if no SF6 gas is detected out, which proves that the on-load tapping switch does not leak the insulating oil. The method determines the problem of oil leakage of the on-load tapping switch by using an indirect method to avoid occurrence of misjudgment accidents, can judge failure property and approximate position inside equipment to provide reliable scientific basis for maintenance work, reduce unplanned power failure and limited loading time of a transformer, greatly reduce economic expenditure on manpower, physical resources and materials, and reduce labor intensity, insecurity factors and the like.

The invention discloses an intelligent-alarming and anti-lost auxiliary walking stick for an old person, and relates to the technical field of intelligent network control equipment. The auxiliary walking stick comprises a walking stick body; supporting legs are fixedly connected to the bottom of the walking stick body; and a movable groove is formed in the middle of the walking stick body. According to the intelligent-alarming and anti-lost auxiliary walking stick for the old person, a dielectric plate is used in cooperation with a counterweight block; when the old person uses the walking stick, the palm presses down a pressing block, a circuit of a magnetic pushing device is connected, and at the moment, the counterweight block is fixed in the middle under the action of a magnetic sheet and the magnetic pushing device; and when the old person falls down, the palm is released, at the moment, the circuit of the magnetic pushing device is disconnected, the counterweight block deflects tothe side surface under the action of gravity, the dielectric plate is moved, the relative area of a positive electrode plate and a negative electrode plate is changed, and an alarm produces a sound and light for reminding passersby to obtain help, so that the effect of giving an alarm is achieved, the old person can timely obtain rescue, and injury aggravation of the old person is avoided.

The invention discloses a single-face automatic feeding four-column cutting machine. The single-face automatic feeding four-column cutting machine comprises a workbench, a lifting rod and a container.Fixing supports are arranged on the two sides of a cutting mechanism, and fixing buckles are arranged below the cutting mechanism. A motor is arranged on the inner surface of the base, and a carryingtable is arranged on one side of the base. Rotation rods are arranged on the outer surface of the container, and mechanical arms are arranged at one ends of the rotation rods. Sharpening blocks are arranged in the cutting mechanism. According to the single-face automatic feeding four-column cutting machine, the fixing buckles, the carrying table, the mechanical arms and the sharpening blocks arearranged, to-be-cut materials on the workbench can be fixed, it is guaranteed that the materials are flatly placed on the workbench, cutting is convenient, the cut materials can be received, automaticfeeding can be conducted, and the work efficiency is improved; and a cutting blade can be ground, the sharpness of the cutting blade is guaranteed, the cutting effect is ensured, the single-face automatic feeding four-column cutting machine is applicable to different work conditions, and better using prospects are brought.

The invention relates to a hydraulic controlled electricity-saving multifunctional water dispenser. Two ends of an electromagnetic valve are connected to a water inlet pipe, the end part of the water inlet pipe is connected to the top of a liner, the wall of the liner is provided with a temperature controller and electric heating wires, one side of the liner is connected with a stainless steel tube, a liquid level controller is arranged in the stainless steel tube, the top side of the stainless steel tube and the top side of the liner are connected with an air outlet pipe at the same time, the bottom of the liner is connected with a water outlet pipe to a hot water outlet valve, the upper part of a hot water valve at the front side of a dispenser body is connected with a micro switch, the front upper part of the dispenser body is provided with a heating insulation indicating lamp, the side wall of the dispenser body is provided with a timer, and the upper part of the back wall of the dispenser body is provided with a liquid level adjusting switch; and one end of a timer switch is connected with a 220V power supply, the other end of the timer switch is connected with the micro switch, the micro switch 21 is connected with a temperature adjustment controller, one path of the temperature controller is connected with a red lamp of heating indicating lamps, the other path of the temperature controller is connected with a green lamp of controlled heat insulation indicating lamps, the joint of two paths of power supply is connected with a music alarm, and the music alarm is connected with a liquid level control switch, the liquid level adjusting switch and the electromagnetic valve which are different.

The invention discloses a wearable communication headset and a communication method based on the communication headset. The communication headset comprises a headset part and a wristband part, wherein the headset part is detachably embedded in the wristband part. The headset part comprises a Bluetooth module, a micro-control unit, a communication module, a battery module, a storage unit, a microphone, a loudspeaker, a coder-decoder and a display screen. After the communication module is activated for the first time, SIM (Subscriber Identity Module) card information of a mobile phone is obtained, and own number information is set as the SIM card information of the mobile terminal through the mobile terminal; and when Bluetooth connection between the Bluetooth module and the mobile terminal is disconnected and the mobile terminal has a call demand, the SIM card information of the mobile terminal is directly accessed to a mobile communication network to finish the call. According to the communication headset provided by the invention, on one hand, users do not need to purchase a new SIM card for the communication headset in addition, so that the economic spending of the users can be reduced, and on the other hand, the communication headset can facilitate the users to dial when they do not carry mobile phones, and the users do not miss calls and short messages of the SIM card of the mobile terminal.

The invention provides a base station information supervising system which comprises a user information management module and a base station information management module. Users are divided into administrators and maintainers by the user information management module, different limits of authority are assigned to the administrators and the maintainers, and the administrators and the maintainers can have access to the base station information management module and conduct corresponding operation only after being verified through the user information management module and proved to be legal. The base station information management module comprises a base station information checking module, a base station information modification module, a base station environment parameter inquiry module and a base station working condition inquiry module. Conventional base station information such as names and addresses of the base station information checking module corresponds to conventional base station information such as names and addresses of the base station information modification module. The base station environment parameter inquiry module can inquire the base station temperature, the base station humidity, the external temperature, the external humidity, the external wind speed and the external wind direction. The base station working condition inquiry module can inquire the base station power, the base station unlink flow and the base station downlink flow.

The invention discloses multicolor printing and dyeing equipment for textile fabric production, and relates to the technical field of fabric production. The equipment aims to solve the problems that an existing dyeing device cannot be used for simultaneously dyeing the two sides of a fabric and a same machine cannot be used for conveniently dyeing the fabric in different colors. The equipment comprises a bottom plate, a first supporting plate is fixedly connected to one side of the top of the bottom plate, and a second supporting plate is arranged on the side, away from the first supporting plate, of the top of the bottom plate; a top plate is arranged on the top of the first supporting plate and the top of the second supporting plate, and two moving devices are movably sleeved with the second supporting plate; two printing and dyeing devices are installed on the sides, close to the first supporting plate, of the moving devices, and discharging devices are arranged on the printing anddyeing devices. The equipment is novel in design and easy to operate, the two sides of the fabric can be conveniently dyed at the same time, dyeing colors can be replaced, there is no need to use other machines for operation, and the economic expenditure of enterprises is reduced.

The invention discloses a multifunctional multielement voltage control current divider of a storage battery, which consists of a plurality of constant-voltage current division load unit circuits with different functional units through parallel connection and combination, wherein the constant-voltage current division load unit circuits consist of three parts: voltage division circuits, voltage stabilizing light-load current division circuits and current enlarging heavy-load circuits through electric connection. Current division units in different element types are combined for use through selecting the types of voltage division resistors and the types of the small-current light-load elements and large-current heavy-load elements according to the maintenance requirements of the storage battery, various kinds of functional current division are automatically realized according to the intensity of the voltage of the storage battery for regulating, monitoring and controlling the static, discharging, charging and restoring features such as voltage, current, balance degree and the like of each storage battery, the balance problems of the storage battery pack of deep discharging activation balance, fast balancing, balanced monitoring, deep charging balance, over charging balance restoration and the like of novel and old serial connection storage battery packs are integrally solved, the goal of greatly prolonging the service life of the storage battery is reached, and the invention has the characteristics of low cost, wide purpose, convenient use, huge requirements and the like.