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A Dissimilatory Iron-Reducing Bacteria and Its Application

A technology of dissimilating iron and bacteria strains, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve problems affecting migration and transformation, and achieve the effect of strong practical application value

Inactive Publication Date: 2018-05-01
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, some Fe(III) reducing bacteria can also reduce toxic heavy metals and metalloids such as Mn(IV), Cr(VI), As(V), thus affecting the migration and transformation of these elements in the natural environment

Method used

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  • A Dissimilatory Iron-Reducing Bacteria and Its Application
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  • A Dissimilatory Iron-Reducing Bacteria and Its Application

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Effect test

Embodiment 1

[0017] Embodiment 1: Isolation, screening and identification of bacterial strains

[0018] (1) Material preparation:

[0019] 1. The experimental water samples were taken from the high-arsenic pollution area of ​​Hangjinhou Banner, Hetao Plain, Inner Mongolia.

[0020] 2. Medium:

[0021] IRM liquid medium: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO4 0.6g / L, yeast extract 0.5g / L; add sodium acetate 20mmol / L, iron citrate 20mmol / L, medium pH 6.7; use high-purity nitrogen to deoxygenate, high temperature and high pressure sterilization.

[0022] IRM solid medium: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO4 0.6g / L, yeast extract 0.5g / L, add sodium acetate 20mmol / L, iron citrate 20mmol / L, medium pH 6.7; add 1.8wt% agar, use high-purity nitrogen to deoxygenate, High temperature and high pressure sterilization.

[0023] LB liquid medium: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, distilled water 1000mL, pH 7.0, deoxygenated with high-purity nitrogen,...

Embodiment 2

[0041] Application of Example 2 Klebsiella oxytoca (Klebsiella oxytoca) IMFRCUG-1 in reducing Fe(III)

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Abstract

The invention belongs to the field of environmental biotechnology, and in particular relates to a dissimilating iron-reducing bacterium and its application. The dissimilating iron-reducing bacteria provided by the present invention is Klebsiella oxytoca IMFRCUG‑1, which was deposited in the China Center for Type Culture Collection on May 27, 2015, with the preservation number CCTCC NO: M 2015332. The Klebsiella oxytoca IMFRCUG-1 of the present invention has a strong reduction function of Fe(III) reduction by dissimilation, and can efficiently reduce Fe(III) in iron citrate and montmorillonite. The invention broadens people's thinking on the application and research of Klebsiella oxytoca in its function, provides new materials for dissimilating iron-reducing bacteria in the treatment of environmental pollutants, and has strong practical application value .

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a dissimilating iron-reducing bacterium and its application. Background technique [0002] Iron is the most abundant transition metal element on Earth. The iron cycle process dominated by ferrous iron (Fe(II))-ferric iron (Fe(III)) in the surface environment is considered to regulate the global organic carbon burial, organic pollutants / heavy metal migration and transformation, and biogenic elements. One of the key factors of geological processes such as migration and environmental changes. Microorganisms are the core participants in the iron cycle on the surface, and their action processes are mainly reflected in two ways: assimilation and dissimilation. Among them, dissimilation, which is characterized by iron oxidation or reduction outside the cell, is an important driving force of the iron cycle. [0003] Dissimilatory iron-reducing bacteria refer to a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/22
Inventor 李平王艳红姜舟戴欣月张睿洪文涛
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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