A Dissimilatory Iron-Reducing Bacteria and Its Application

A technology of dissimilating iron and bacteria strains, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve problems affecting migration and transformation, and achieve the effect of strong practical application value

Inactive Publication Date: 2018-05-01
CHINA UNIV OF GEOSCIENCES (WUHAN)
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, some Fe(III) reducing bacteria can also reduce toxic heavy metals and metalloids such as Mn(IV), Cr(VI), As(V), thus affecting the migration and transformation of these elements in the natural environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Dissimilatory Iron-Reducing Bacteria and Its Application
  • A Dissimilatory Iron-Reducing Bacteria and Its Application
  • A Dissimilatory Iron-Reducing Bacteria and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Isolation, screening and identification of bacterial strains

[0018] (1) Material preparation:

[0019] 1. The experimental water samples were taken from the high-arsenic pollution area of ​​Hangjinhou Banner, Hetao Plain, Inner Mongolia.

[0020] 2. Medium:

[0021] IRM liquid medium: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO4 0.6g / L, yeast extract 0.5g / L; add sodium acetate 20mmol / L, iron citrate 20mmol / L, medium pH 6.7; use high-purity nitrogen to deoxygenate, high temperature and high pressure sterilization.

[0022] IRM solid medium: NaHCO 3 2.5g / L, KCl 0.1g / L, NH 4 Cl 1.5g / L, NaH 2 PO4 0.6g / L, yeast extract 0.5g / L, add sodium acetate 20mmol / L, iron citrate 20mmol / L, medium pH 6.7; add 1.8wt% agar, use high-purity nitrogen to deoxygenate, High temperature and high pressure sterilization.

[0023] LB liquid medium: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, distilled water 1000mL, pH 7.0, deoxygenated with high-purity nitrogen,...

Embodiment 2

[0041] Application of Example 2 Klebsiella oxytoca (Klebsiella oxytoca) IMFRCUG-1 in reducing Fe(III)

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of environmental biotechnology, and in particular relates to a dissimilating iron-reducing bacterium and its application. The dissimilating iron-reducing bacteria provided by the present invention is Klebsiella oxytoca IMFRCUG‑1, which was deposited in the China Center for Type Culture Collection on May 27, 2015, with the preservation number CCTCC NO: M 2015332. The Klebsiella oxytoca IMFRCUG-1 of the present invention has a strong reduction function of Fe(III) reduction by dissimilation, and can efficiently reduce Fe(III) in iron citrate and montmorillonite. The invention broadens people's thinking on the application and research of Klebsiella oxytoca in its function, provides new materials for dissimilating iron-reducing bacteria in the treatment of environmental pollutants, and has strong practical application value .

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a dissimilating iron-reducing bacterium and its application. Background technique [0002] Iron is the most abundant transition metal element on Earth. The iron cycle process dominated by ferrous iron (Fe(II))-ferric iron (Fe(III)) in the surface environment is considered to regulate the global organic carbon burial, organic pollutants / heavy metal migration and transformation, and biogenic elements. One of the key factors of geological processes such as migration and environmental changes. Microorganisms are the core participants in the iron cycle on the surface, and their action processes are mainly reflected in two ways: assimilation and dissimilation. Among them, dissimilation, which is characterized by iron oxidation or reduction outside the cell, is an important driving force of the iron cycle. [0003] Dissimilatory iron-reducing bacteria refer to a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/22
Inventor 李平王艳红姜舟戴欣月张睿洪文涛
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap