Detection of bacterium by utilizing dnaj gene and use thereof

A technology of bacteria and genes, applied in the determination/inspection of microorganisms, recombinant DNA technology, resistance to vector-borne diseases, etc., can solve the problem of large sequence differences (Non-patent document 8, high polymorphism level at the species level).
CN101558168AInactive Publication Date: 2009-10-14GIFU UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GIFU UNIVERSITY
Publication Date
2009-10-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

Disclosed is a method for detecting the species of a bacterium in a simple manner. Specifically disclosed is a method for detecting the species of a bacterium, which can detect at least one bacterial species selected from Staphylococcus species, Streptococcus species, Klebsiella species, Escherichia species, Mycobacterium species, Legionella species, Vibrio species, Bacillus species, Neisseria species, Campylobacter species, Chlamydia species, Chlamydophila species, Mycoplasma species, Listeria species, Salmonella species and Yersinia species, and which comprises the steps of: a) contacting a sample suspected to contain a nucleic acid derived from the bacterium species to be detected with a nucleic acid molecule capable of hybridizing with at least a part of a DnaJ gene derived from the bacterial species; and b) detecting the presence or absence of the hybridization between the nucleic acid molecule and the nucleic acid contained in the sample.
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Description

technical field

[0001] The present invention relates to the detection of bacteria using the DnaJ gene and its utilization. In detail, it relates to a method for detecting bacteria using the DnaJ gene, a nucleic acid molecule used in the method, and applications of the method and the nucleic acid molecule in diagnosis and identification . Background technique

[0002] For a long time, the detection and identification of various bacteria such as pathogenic bacteria requires the use of special reagents and skilled techniques, and the use of special reagents and skilled techniques for the cultivation of target bacteria. The growth of pathogenic bacteria is accompanied by a certain degree of danger. In addition, when considering pathogenic bacteria with various possibilities, it is necessary to cultivate them under various conditions. Furthermore, in order to identify bacterial species, it is necessary to examine in detail, for example, biochemical characteristics. Therefore, t...

Claims

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