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Chlorimuron-ethyl degrading bacterium, soil bioremediation agent based on same, and application thereof

A technology of chlorimuron-methyl and soil biology, which is applied in the biological field, can solve the problems affecting technology development and low degradation rate, and achieve the effects of eliminating pesticide residues, good degradation effect and low production cost

Inactive Publication Date: 2010-08-11
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microbial remediation is affected by many factors in practical applications, so the degradation rate is often low, which affects the development of this technology

Method used

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  • Chlorimuron-ethyl degrading bacterium, soil bioremediation agent based on same, and application thereof
  • Chlorimuron-ethyl degrading bacterium, soil bioremediation agent based on same, and application thereof
  • Chlorimuron-ethyl degrading bacterium, soil bioremediation agent based on same, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The enrichment, separation, purification and preparation of bacterial suspension of chlorimsulfuron-methyl-degrading bacteria Get 1g of sludge sample (production of chlorimsulfuron-methyl pesticide factory outfall sludge) and add 50mL inorganic salt culture medium In the Erlenmeyer flask, take 4mL from the prepared 250mg / L chlorimuron-methyl solution and directly add it to the Erlenmeyer flask as the only nitrogen source (5mg / L) for microbial growth, and cultivate it on a shaker for 2d (30°C, 60r After being transferred to the inorganic salt medium containing chlorimuron-methyl 20mg / L and culturing for 2d, then insert the above-mentioned inorganic salt of cultivating 2d on the chlorimuron-methyl plate medium containing concentration of 250mg / L Culture medium 0.1mL, culture in 35°C incubator for 2 days after coating, pick out a single colony and repeatedly transplant and streak 3 to 4 times on the plate medium for purification, dip a little of the purified 2N3 ...

Embodiment 2

[0029] Embodiment 2: degradation condition:

[0030] 1. The effect of pH value on the degradation of chlorimuron-methyl by strain 2N3

[0031]Be 2% inoculation amount by volume percentage, the 2N3 bacterium suspension that embodiment 1 prepares is inserted in the inorganic salt medium that chlorimuron-methyl concentration is 100mg / L, adjusts the pH value of culture medium respectively to be 5,6, On 7, 8, 9, and 10, cultivate at 30°C and 200 rpm on a shaker, and measure the content of chlorimuron-methyl at 4, 8, 10, 12, 24, and 36 hours, respectively.

[0032] The inorganic salt culture medium is glucose 5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, K 2 HPO 4 0.5g, NaCl 0.2g, add 1000mL distilled water, the pH value is natural.

[0033] The experimental results of the pH value on the degradation of chlorimuron-methyl by strains showed that when the pH value was greater than 8.0, the degradation efficiency of chlorimuron-methyl decreased gradually as the pH value increased. ...

Embodiment 3

[0047] Embodiment 3: Bacteria agent preparation test

[0048] In the slant test tube strain of chlorimuron-methyl-degrading bacteria 2N3, use an inoculation loop to inoculate a little bacteria on the plate medium, cultivate at 35°C for 10 hours, then use an inoculation loop to inoculate a little bacteria in the LB medium, 30 Cultivate with shaking at ℃ to the logarithmic phase, the formula of the plate culture medium is: NaCl 15g, peptone 10g, beef extract 5g, agar 15g, add 1000mL distilled water; the LB culture medium is : NaCl 10g, yeast powder 5g, tryptone 5g, add 1000ml of distilled water; put 10mL of the cultured strain into the seed bottle, shake and cultivate to the logarithmic phase, the medium of the 1000mL seed bottle is: glucose 8g, MgSO 4 ·7H 2 O 0.7g, K 2 HPO 4 4g, NaCl 2g, CaCO 3 5g, 0.4g of yeast extract, add 1000mL of water, the pH value of the medium is 6.0-7.0;

[0049] Add 15 mL of the cultured strains above to 1000 g of fermentation medium consisting ...

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Abstract

The invention relates to a chlorimuron-ethyl pesticide residue degrading bacterium which is named klebsiella 2N3 (Klebsiella jilinsis H.Zhang 2N3) with the storage No. of CCTCC NO: M 209248 in China Center for Type Culture Collection (CCTCC), a soil bioremediation agent prepared from the chlorimuron-ethyl pesticide residue degrading bacterium, and application thereof. The chlorimuron-ethyl pesticide residue degrading bacterium is a bacterial strain which has strong degradation capacity to chlorimuron-ethyl and is obtained by carrying out enrichment culture and separation on sludge at a drainage outlet of an insecticide factory for producing chlorimuron-ethyl, the bacterial strain grows by using the chlorimuron-ethyl as the unique nitrogen source, and the activity of the bacterium in a liquid nutrient medium is increased by the induction of the chlorimuron-ethyl so as to improve the degradation capacity to the chlorimuron-ethyl. The preparation method for the bacterium is simple, low in cost and convenient to use, and can be spread in the soil, and the degradation effect in the soil can reach 82.6 percent; therefore, the chlorimuron-ethyl pesticide residue degrading bacterium has good application prospect.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and specifically relates to a chlorimuron-methyl pesticide residue-degrading bacterium and a soil bioremediation bacterial agent prepared by using the bacterium and its application. It uses microorganisms to degrade chemical pesticide residues, and is suitable for green production in modern agricultural production. Production and processing of pollution-free agricultural products. Background technique: [0002] Chlorsulfuron-methyl is a sulfonylurea herbicide developed by Dubang Company in the United States in 1984. It is mainly used to control some weeds in soybean fields. These herbicides have high herbicidal activity and low toxicity to humans and animals. However, some varieties such as chlorimuron-methyl remain in the soil for a long time, and have obvious phytotoxic effects on subsequent sensitive crops such as corn, sorghum, rape, melons, potatoes, sugar beets, etc., which limits its...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/22A62D101/04
Inventor 张浩汪佳秀潘洪玉穆文辉张祥辉
Owner JILIN AGRICULTURAL UNIV
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