Complex microbial inoculant for promoting wheat growth and application thereof

A compound bacterial agent and wheat technology, applied in the direction of plant growth regulator, plant growth regulator, application, etc., can solve the problems of unsatisfactory, single function, etc., and achieve the effect of reducing the amount of use, promoting plant growth, and increasing plant height

Active Publication Date: 2019-12-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since this technology only contains one kind of nitrogen-fixing bacteria, the function is relatively simple. Although it can fix nitrogen and dissolve phosphorus to a certain extent, it still cannot meet the needs of large amounts of nitrogen, phosphorus, and potassium for plant growth.

Method used

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  • Complex microbial inoculant for promoting wheat growth and application thereof
  • Complex microbial inoculant for promoting wheat growth and application thereof
  • Complex microbial inoculant for promoting wheat growth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The screening and identification of embodiment 1 nitrogen-fixing bacteria

[0055] 1. Screening of nitrogen-fixing bacteria

[0056] 1. Primary screening of nitrogen-fixing bacteria

[0057] In September 2017, wheat plants that had grown for about 7 months were collected from the farmland in Yangling District, Shaanxi Province, and the loosely bound soil at the roots of the wheat was shaken vigorously. Cut off the root of the plant and put it into a 10mL sterilized centrifuge tube, inject 5mL of sterile water and perform ultrasonic treatment to dissolve the rhizosphere soil closely combined with the root of the plant in the water. The collected soil samples are yellow loam, with 24.03mg / kg of available phosphorus, 126.17mg / kg of available potassium, 56.28mg / kg of alkaline nitrogen, 21.06g / kg of organic matter, and pH=8.27.

[0058] Make a series of 10-fold dilutions of the aqueous solution, and take 5 μL of the dilution to be 10 times -5 、10 -6 and 10 -7 The soil s...

Embodiment 2

[0084] Screening and Identification of Example 2 Potassium Solubilizing Bacteria

[0085] 1. Screening of Potassium Solubilizing Bacteria

[0086] 1. Preliminary screening of potassium decomposing bacteria

[0087] In September 2017, wheat plants that had grown for about 7 months were collected from the farmland in Yangling District, Shaanxi Province, and the loosely bound soil at the roots of the wheat was shaken vigorously. Cut off the root of the plant and put it into a 10mL sterilized centrifuge tube, inject 5mL of sterile water and perform ultrasonic treatment to dissolve the rhizosphere soil closely combined with the root of the plant in the water. The collected soil samples are yellow loam, with 24.03mg / kg of available phosphorus, 126.17mg / kg of available potassium, 56.28mg / kg of alkaline nitrogen, 21.06g / kg of organic matter, and pH=8.27.

[0088] Make a series of 10-fold dilutions of the aqueous solution, and take 5 μL of the dilution to be 10 times -5 、10 -6 and ...

Embodiment 3

[0115] The screening and identification of embodiment 3 phosphate-dissolving bacteria

[0116] 1. Screening of Phosphate-Solubilizing Bacteria

[0117] 1. Primary screening of phosphate-solubilizing bacteria

[0118] In September 2017, wheat plants that had grown for about 7 months were collected from the farmland in Yangling District, Shaanxi Province, and the loosely bound soil at the roots of the wheat was shaken vigorously. Cut off the root of the plant and put it into a 10mL sterilized centrifuge tube, inject 5mL of sterile water and perform ultrasonic treatment to dissolve the rhizosphere soil closely combined with the root of the plant in the water. The collected soil samples are yellow loam, with 24.03mg / kg of available phosphorus, 126.17mg / kg of available potassium, 56.28mg / kg of alkaline nitrogen, 21.06g / kg of organic matter, and pH=8.27.

[0119] Make a series of 10-fold dilutions of the aqueous solution, and take 5 μL of the dilution to be 10 times -5 、10 -6 an...

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Abstract

The invention discloses a complex microbial inoculant for promoting wheat growth and an application thereof. The complex microbial inoculant is prepared by mixing and fermenting an Azotobacter chroococcum N24 bacterial solution, a rhizobium radiobacter J16 bacterial solution and a Klebsiella quassicola P5 bacterial solution. According to the invention, nitrogen-fixing bacteria N24, potassium bacteria J16 and phosphate solubilizing bacteria P5 are separated from rhizosphere soil of wheat, the complex microbial inoculant prepared by mixing and fermenting the three bacterial solutions simultaneously has the functions of nitrogen fixation, phosphorus dissolution and potassium dissolution, the contents of available nitrogen, available phosphorus and available potassium in soil can be greatly increased, the plant height, the fresh weight, the dry weight, the nitrogen content, the phosphorus content and the potassium content of plants are remarkably increased, the plant growth is promoted, the use amount of chemical fertilizers is reduced, the crop yield is increased, and the method has important significance in agricultural production.

Description

technical field [0001] The invention relates to the technical field of plant rhizosphere growth-promoting bacteria, in particular to a composite bacterial agent for promoting the growth of wheat and its application. Background technique [0002] Nitrogen, phosphorus, and potassium are essential nutrients for plant growth and metabolic activities, and play a key role in the high yield of crops. Relevant literature shows that 95% of phosphorus in the soil combines with calcium, aluminum, iron and other ions to form insoluble compounds; while potassium and alkali metals such as sodium and calcium also exist in the form of aluminosilicate, which reduces the solubility of potassium. Therefore, the activated phosphorus and potassium elements that plants can absorb and utilize in the soil are less. In addition, although a large amount of chemical fertilizers are put into farmland every year, the utilization rate of these chemical fertilizers by plants is only about 35%, and the ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/065C12R1/41C12R1/22
CPCC12N1/20A01N63/00C12R2001/065C12N1/205C12R2001/22C12R2001/41Y02A40/10
Inventor 李哲斐王娟娟
Owner NORTHWEST A & F UNIV
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