Application of elsholtzia volatile oil in sterilizing and/or anti-bacteria and antibiotic resistant bacteria and preparation method thereof
A technology of volatile oil and drug-resistant bacteria, which is applied in the fields of application, antibacterial drugs, and pharmaceutical formulations, and can solve the problems that have not yet been seen in the research on the anti-drug-resistant bacteria activity of fenugreek volatile oil
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] Embodiment 1 antibacterial effect of the volatile oil of Chinese herbaceae
[0021] 1 Materials and methods
[0022] 1.1 Materials:
[0023] (1) Tested strains: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa.
[0024] (2) Culture medium: nutrient broth medium, Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.
[0025] 1.2 Experimental method
[0026] 1.2.1 Activation of strains: Take 3 clean test tubes, add 5 mL of nutrient broth medium respectively, seal with silica gel plugs, and sterilize at 121°C for 30 minutes. Let the sterilized test tube cool down on the ultra-clean workbench, then use an inoculation loop to take an appropriate amount of 3 kinds of bacteria into the test tube, mix them evenly, and place them in the incubator for 18-24 hours to observe the results. Among them, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were placed in a 37°C incubator.
[0027] 1.2.2 Oxford cup method (tube and dish method) to screen t...
Embodiment 2
[0032] Embodiment 2 antifungal effect of the volatile oil of Rhizoma rudis
[0033] 1 Materials and methods
[0034] 1.1 Materials:
[0035] (1) Tested strain: Candida albicans.
[0036] (2) Culture medium: nutrient broth medium, improved Martin medium, Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.
[0037] 1.2 Experimental method
[0038] 1.2.1 Activation of strains: Take a clean test tube, add 5 mL of modified Martin's medium, seal it with a silica gel plug, and sterilize at 121°C for 30 minutes. Let the sterilized test tube cool down on the ultra-clean workbench, then use an inoculation loop to take an appropriate amount of 1 species of bacteria into the test tube, mix well, and place it in an incubator for 18-24 hours to observe the results. Place Candida albicans at 26.5°C in the incubator.
[0039] 1.2.2 Oxford cup method (tube and dish method) to screen the antibacterial activity of volatile oil of traditional Chinese medicine
[0040] Measure the OD600 value...
Embodiment 3
[0044] Embodiment 3 anti-drug-resistant bacteria effect of the volatile oil of Rhizoma rudis
[0045] 1. Materials and methods
[0046] 1.1 Bacterial Strains
[0047] Drug-resistant Staphylococcus aureus (MRSA), bacterial number: BAA-1717; drug-resistant Klebsiella, bacterial number: BAA-1705; drug-resistant Pseudomonas aeruginosa, bacterial number: BAA-2110; Drug-resistant Enterococcus faecium, strain number: BAA-2320; drug-resistant Acinetobacter baumannii, strain number: 19606; both were purchased from the American Type Culture Collection (ATCC). Drug-resistant entero-invasive Escherichia coli, bacteria number: 186731, was purchased from Beijing Beina Chuanglian Biotechnology Research Institute. Beta-hemolytic streptococci were purchased from China General Microorganism Culture Collection and Management Center.
[0048] 1.2 Test reagents
[0049] table 3
[0050]
[0051] 1.3 Test equipment
[0052] Table 4
[0053]
[0054] 1.4 Test method
[0055] Add tryptica...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com