Application of elsholtzia volatile oil in sterilizing and/or anti-bacteria and antibiotic resistant bacteria and preparation method thereof

A technology of volatile oil and drug-resistant bacteria, which is applied in the fields of application, antibacterial drugs, and pharmaceutical formulations, and can solve the problems that have not yet been seen in the research on the anti-drug-resistant bacteria activity of fenugreek volatile oil

Inactive Publication Date: 2018-09-25
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant literature report on the anti-drug-resistant bacteria activity of the volatile oil of Arthia officinalis

Method used

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  • Application of elsholtzia volatile oil in sterilizing and/or anti-bacteria and antibiotic resistant bacteria and preparation method thereof
  • Application of elsholtzia volatile oil in sterilizing and/or anti-bacteria and antibiotic resistant bacteria and preparation method thereof
  • Application of elsholtzia volatile oil in sterilizing and/or anti-bacteria and antibiotic resistant bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 antibacterial effect of the volatile oil of Chinese herbaceae

[0021] 1 Materials and methods

[0022] 1.1 Materials:

[0023] (1) Tested strains: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa.

[0024] (2) Culture medium: nutrient broth medium, Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.

[0025] 1.2 Experimental method

[0026] 1.2.1 Activation of strains: Take 3 clean test tubes, add 5 mL of nutrient broth medium respectively, seal with silica gel plugs, and sterilize at 121°C for 30 minutes. Let the sterilized test tube cool down on the ultra-clean workbench, then use an inoculation loop to take an appropriate amount of 3 kinds of bacteria into the test tube, mix them evenly, and place them in the incubator for 18-24 hours to observe the results. Among them, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were placed in a 37°C incubator.

[0027] 1.2.2 Oxford cup method (tube and dish method) to screen t...

Embodiment 2

[0032] Embodiment 2 antifungal effect of the volatile oil of Rhizoma rudis

[0033] 1 Materials and methods

[0034] 1.1 Materials:

[0035] (1) Tested strain: Candida albicans.

[0036] (2) Culture medium: nutrient broth medium, improved Martin medium, Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.

[0037] 1.2 Experimental method

[0038] 1.2.1 Activation of strains: Take a clean test tube, add 5 mL of modified Martin's medium, seal it with a silica gel plug, and sterilize at 121°C for 30 minutes. Let the sterilized test tube cool down on the ultra-clean workbench, then use an inoculation loop to take an appropriate amount of 1 species of bacteria into the test tube, mix well, and place it in an incubator for 18-24 hours to observe the results. Place Candida albicans at 26.5°C in the incubator.

[0039] 1.2.2 Oxford cup method (tube and dish method) to screen the antibacterial activity of volatile oil of traditional Chinese medicine

[0040] Measure the OD600 value...

Embodiment 3

[0044] Embodiment 3 anti-drug-resistant bacteria effect of the volatile oil of Rhizoma rudis

[0045] 1. Materials and methods

[0046] 1.1 Bacterial Strains

[0047] Drug-resistant Staphylococcus aureus (MRSA), bacterial number: BAA-1717; drug-resistant Klebsiella, bacterial number: BAA-1705; drug-resistant Pseudomonas aeruginosa, bacterial number: BAA-2110; Drug-resistant Enterococcus faecium, strain number: BAA-2320; drug-resistant Acinetobacter baumannii, strain number: 19606; both were purchased from the American Type Culture Collection (ATCC). Drug-resistant entero-invasive Escherichia coli, bacteria number: 186731, was purchased from Beijing Beina Chuanglian Biotechnology Research Institute. Beta-hemolytic streptococci were purchased from China General Microorganism Culture Collection and Management Center.

[0048] 1.2 Test reagents

[0049] table 3

[0050]

[0051] 1.3 Test equipment

[0052] Table 4

[0053]

[0054] 1.4 Test method

[0055] Add tryptica...

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Abstract

The invention discloses application of elsholtzia volatile oil in anti-bacteria and antibiotic resistant bacteria. The elsholtzia volatile oil is extracted by steam distillation. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of staphylococcus, escherichia coli, candida, pseudomonas, enterobacter, streptococcus and the like and fungi. The elsholtzia volatile oil has certain functions of killing and/or inhibiting growth on bacteria of drug-resistant staphylococcus, drug-resistant escherichia coli, drug-resistant enterobacter, drug-resistant klebsiella, drug-resistant pseudomonas, drug-resistant acinetobacter, drug-resistant streptococcus, and drug-resistant enterococcus and the like. The elsholtzia volatile oil can be used in industries offood, medicine, health care products, cosmetics and the like and can be used for preparing various forms of products with antibacterial and antibiotic resistant bacteria functions, and belongs to thenew application field of the elsholtzia volatile oil.

Description

technical field [0001] The invention relates to the technical field of food, medicine and chemical industry, and in particular to the application and preparation method of volatile oil of fenugreek for sterilizing, or inhibiting and resisting drug-resistant bacteria. Background technique [0002] Moslae Herba is the dry aerial part of Mosla chinensis Maxim. or Mosla chinensis 'Jiangxiangru' of Lamiaceae plant. It is one of the key medicinal materials developed and researched in my country, and also one of the seasonings commonly used in daily food. It is a dual-purpose traditional Chinese medicine for medicine and food. It is warm in nature, pungent in taste, and has the effects of sweating, relieving the exterior, dispelling dampness, and harmonizing the middle. It is often used clinically to treat summer-dampness cold, aversion to cold and fever, headache without sweating, abdominal pain, vomiting and diarrhea. The main chemical component of fenugreek is volatile oil, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P31/04A61K8/92A61Q19/00A23L33/105
CPCA23L33/105A23V2002/00A61K8/922A61K36/53A61K2236/331A61K2800/10A61P31/04A61Q19/00A23V2200/30A23V2250/21Y02A50/30
Inventor 王雅琪杨明梁新丽伍振峰郑琴黄小英张小飞
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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