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Method for detecting oil content of microalgae

A technology of oil content and microalgae, which is applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of high equipment requirements, time-consuming and laborious, etc., and achieve the effect of simple and convenient process, good application effect and fast and simple detection

Inactive Publication Date: 2014-06-11
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Among them, the traditional extraction method and chromatography are time-consuming and laborious, and have high requirements for equipment; dyeing and colorimetry are time-saving, but can only determine the relative content of microalgae oil

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  • Method for detecting oil content of microalgae
  • Method for detecting oil content of microalgae
  • Method for detecting oil content of microalgae

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Embodiment

[0032] Materials and Methods:

[0033] 1) Experimental algal species The experimental algal species Platymonas subcordiformis was obtained from the microalgae culture laboratory of the Chinese Academy of Fishery Sciences-Yellow Sea Fisheries Research Institute.

[0034] 2) The formula of the basic culture medium used in the culture condition experiment is NaNO 3 (120mg / L), KH 2 PO 4 (4mg / L), FeC 6 h 5 o 7 (5mg / L), vitamin B 1 (200μg / L), vitamin B 12 (200μg / L). The microalgae culture was carried out in a photochemical incubator (GXZ intelligent type, Ningbo Jiangnan Instrument Factory) by indoor constant temperature culture method. Set the light intensity to 120 μmol m -2 ·s -1 , temperature 20±1℃, photoperiod 12h(L):12h(D).

[0035] 3) Experimental method

[0036] 3.1) Experimental design Under the above-mentioned culture conditions, algae species in the logarithmic growth phase were inoculated into the above-mentioned culture medium as experimental materials, and ...

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Abstract

The invention relates to lipid producing microalgae, in particular relates to a method for detecting the oil content of microalgae, comprising the following steps of: 1) taking microalgae-cultivated algae liquid, and computing the quantity concentration C of microalgae cells; 2) observing the lipid of the microalgae: adopting a BODIPY (boron-dipyrromethene) 505 / 515 staining fluorescent recording method; staining for 1-2min by adopting dye use liquid with concentration of 1-2mM, and observing and recording accumulation change conditions of single-celled lipid in a photographing way by using excitation light with wave length of 488nm under a fluorescence microscope; recording the number (n) of single-celled oil drop grains according to a photograph, detecting the diameter (d) of each oil drop, and computing the volume of each oil drop according to a ball volume formula, wherein the content of each cell lipid is the sum of the volume of all the oil drops; and computing the lipid content of the algae liquid in unit volume according to the quantity concentration C of the microalgae and the lipid content of a single cell: Vt=CV, wherein C is the concentration of tetraselmis cells, V is the lipid volume of the single cell, and Vt is the lipid content in the unit volume.

Description

technical field [0001] The invention relates to lipid-producing microalgae, in particular to a method for detecting the oil content of the microalgae. Background technique [0002] As an important renewable resource, algae has outstanding characteristics such as wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high yield. The further development and utilization of algae, especially microalgae, will provide new source of resources. Microalgae has great potential as an energy raw material. Its cells contain unique primary or secondary metabolites, complex chemical composition, and solar energy conversion efficiency can reach 3.5%. It is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. Fatty acids obtained from microalgae can be converted into fatty acid methyl esters, namely biodiesel; under the action of zeolite catalysts, microalgae can produce gasoline-type ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01B11/08G01N15/10
Inventor 叶乃好徐东张晓雯
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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