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Soil microalgae of mining area, separation and purification method for soil microalgae of mining area and application of soil microalgae of mining area

A technology for separation and purification of microalgae, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of phosphorus and other pollutants that are difficult to deal with, such as treatment costs, and achieve easy operation and reduced treatment costs , Good absorption and absorption capacity

Pending Publication Date: 2020-10-23
WUHAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, the present invention aims to propose a kind of mining area soil microalgae, which is purified from the mining area soil crust with high heavy metal content, and its tolerance to heavy metals and adaptability to extreme environments are better, and more It solves the problem that the heavy metals, phosphorus and other pollutants in the existing polluted soil and water are difficult to deal with and the treatment cost is high

Method used

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  • Soil microalgae of mining area, separation and purification method for soil microalgae of mining area and application of soil microalgae of mining area
  • Soil microalgae of mining area, separation and purification method for soil microalgae of mining area and application of soil microalgae of mining area
  • Soil microalgae of mining area, separation and purification method for soil microalgae of mining area and application of soil microalgae of mining area

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Embodiment 1

[0034] A kind of soil microalgae in a mining area is the soil microalgae separated and purified from the biological crust on the surface of the tailings, which is specifically separated and purified by the following method:

[0035] 1) Shovel the biological crust on the surface of the tailings, crush it lightly, and pass through a 100-mesh sieve to obtain a mixture of algae and moss in the crust. Shoveling from the tailings in the area;

[0036] 2) Place the algae and moss mixture in step 1) in a sterile conventional algae liquid medium, and culture it in a constant temperature light incubator at 25° C. with a light intensity of 10000 lx for 48 hours, then separate to obtain the algae liquid;

[0037] 3) Take 1 to 5 drops of the algae liquid in step 2) in the first solid medium, so that the sample liquid evenly covers the surface of the first solid medium, seal the sealing film and place it at 25°C, with a light intensity of 10000lx, 14: Store and cultivate in a constant temp...

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Abstract

The invention provides soil microalgae of a mining area, a separation and purification method for the soil microalgae of the mining area and application of the soil microalgae of the mining area. Thesoil microalgae of the mining area are separated and purified from biological crusts of surfaces of tailings and comprise one or more selected from Chlorella, Cylindrotheca, Diatom, Nitzschia, Schizochytrium, Dunaliella, Scenedesmus, Nannochloropsis oculata, Chlamydomonas, Tetraselmis and Eudovina. The soil microalgae of the mining area, provided by the invention, are separated and purified from the biological crusts of the surfaces of the tailings; the obtained microalgae are relatively extensive in variety; and the obtained microalgae have relatively good adsorption and absorption capabilityto contaminants such as heavy metals and phosphorus in contaminated soil and water and then can effectively treat the contaminants such as the heavy metals and phosphorus in the contaminated soil andwater, so that the treatment cost of the contaminants such as the heavy metals and phosphorus in the contaminated soil and water is reduced greatly.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a soil microalgae in a mining area and its separation and purification method and application. Background technique [0002] Soil microalgae, as an important part of soil microorganisms, refer to the algae groups of various groups such as soil surface, subsurface, and soil formation, succession, and soil composition, such as lithophytic, aquatic-terrestrial algae, etc. , paddy fields and soils with high organic matter content are most widely distributed. [0003] Soil microalgae are rich in species. In the environmental field, microalgae, as one of the organisms with adsorption capacity, has the advantages of high photosynthetic rate, fast reproduction, and strong adaptability to the environment. Nowadays, there are a large number of research reports on microalgae as biosorbents in sewage treatment and soil pollution applications. The oil produced by some microalgae such...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02B09C1/10C02F3/32C12R1/89
CPCC12N1/12C12N1/02B09C1/10C02F3/322B09C2101/00C12R2001/89C12N1/125
Inventor 夏令黄容霍广铖柳思源杨颖
Owner WUHAN UNIV OF TECH
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