Microbial water purifying agent for puffer fish culture water and preparation method thereof

A microbial reagent, aquaculture water technology, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc. High purification efficiency and easy precipitation

Active Publication Date: 2015-01-14
徐州邦尼生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current aquaculture technology, because the aquaculture water cannot be treated, it is easy to cause problems such as an increase in the incidence of puffer fish, a decrease in survival rate, and a decrease in production.
The conventional solution is to change the water at intervals, but on the one hand, changing the water will increase the production cost, and on the other hand, it will easily cause pufferfish's unsuitable symptoms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of magnetic silica spheres with surface-modified C8 and C18 alkyl chains:

[0025] (1-1) Synthesis of Fe by hydrothermal method 3 o 4 Magnetic nanoparticles: 1 g of FeCl 3 ·6H 2 O, dissolved in 30ml of ethylene glycol, adding 4 grams of anhydrous sodium acetate, the reaction temperature is 200 ° C, the reaction time is 12 hours, and Fe is generated 3 o 4 magnetic nanoparticles;

[0026] (1-2) The above Fe 3 o 4 Wash the magnetic nanoparticles with water to remove water-soluble impurities, and then disperse them in 10 g of water to obtain dispersion A. Take a centrifuge tube, add 3 g of the above dispersion A, add 5 ml of 2M HCl solution and sonicate for 8 minutes, and then use a magnet to separate and remove the green color. Clear liquid, then disperse with 10ml water, collect particles with magnet, remove light green clear liquid, disperse ultrasonically with 5 ml 10wt% trisodium citrate solution, let it stand for 2 hours, re-disperse with 5 ml w...

Embodiment 2

[0033] Basically the same as in Example 1, a microbial water purifier for puffer fish breeding water was prepared, the only difference being that the surface modification agent in step (1-4) was different. In Example 2, 0.3 g of dimethyloctylsilane was used as the surface modifier.

Embodiment 3

[0035]Basically the same as in Example 1, a microbial water purifier for puffer fish breeding water was prepared, the only difference being that the surface modification agent in step (1-4) was different. In Example 3, 0.3 g of octadecyltriethoxysilane was used as the surface modifier.

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PUM

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Abstract

The invention discloses a microbial water purifying agent for puffer fish culture water and a preparation method thereof. The preparation method comprises the following steps: adding 30-40 parts by weight of surface-modified C8 and C18 alkyl-chain magnetic silicon balls into 300-400 parts by weight of water; adding 80-120 parts by weight of microbial reagent, including 60-80 parts of Synechococcus, 50-70 parts of Rhodobacter sphaeroides, 20-30 parts of Nitrosospira, 20-30 parts of Nitrosococcus, 10-30 parts of Pseudomonas stutzeri, 20-30 parts of iron bacteria, 3-6 parts of Thiothrix, 3-6 parts of Beggiatoa, 50-80 parts of Sarcomastigophora, 50-70 parts of amoebae, 30-50 parts of Tetraselmis and 30-40 parts of dunaliella tertiolectra; and adding 60-80 parts by weight of beef extract peptone agar culture medium, and culturing. The microbial water purifying agent can be added into a puffer fish culture tank to achieve the goal of purifying culture tank water.

Description

technical field [0001] The invention relates to a microbial water purifying agent and a preparation method thereof, in particular to a microbial water purifying agent used for pufferfish breeding water and a preparation method thereof. Background technique [0002] In recent years, the rapid advancement of high-efficiency agriculture has led to the rapid development of special aquaculture. Famous, special and high-quality new aquaculture species have reached the level of large-scale production and formed their own characteristics. Puffer, English name: Far east puffers, commonly known as "bubble fish", "blowing belly fish", "river puffer fish", "gas drum fish" (Jiangsu, Zhejiang), "good fish" (Guangdong), "chicken puffer fish" "Bao" (Guangdong), "Turtle Fish" (Guangxi), "Street Fish" (Fujian), "Wax Head" (Hebei), and "Boat Mackerel". Pufferfish are warm temperate and tropical offshore bottom fish, inhabiting In the middle and lower layers of the ocean, a small number of sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F3/325C02F3/34
Inventor 许银亚
Owner 徐州邦尼生物技术有限公司
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