A kind of microbial water purifying agent for pufferfish culture water and preparation method thereof

A technology for microbial reagents and aquaculture water, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problems of decreased production, decreased survival rate, and increased incidence of puffer fish, etc. Achieve the effect of high purification efficiency and easy precipitation

Active Publication Date: 2016-03-30
徐州邦尼生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current aquaculture technology, because the aquaculture water cannot be treated, it is easy to cause problems such as an increase in the incidence of puffer fish, a decrease in survival rate, and a decrease in production.
The conventional solution is to change the water at intervals, but on the one hand, changing the water will increase the production cost, and on the other hand, it will easily cause pufferfish's unsuitable symptoms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of magnetic silicon spheres with surface-modified C8 and C18 alkyl chains:

[0025] (1-1) Synthesis of Fe by hydrothermal method 3 o 4 Magnetic nanoparticles: 1 g of FeCl 3 ·6H 2 O, dissolved in 30ml of ethylene glycol, adding 4 grams of anhydrous sodium acetate, the reaction temperature is 200 ° C, the reaction time is 12 hours, and Fe is generated 3 o 4 magnetic nanoparticles;

[0026] (1-2) the above Fe 3 o 4 Wash the magnetic nanoparticles with water to remove water-soluble impurities, and then disperse them in 10 g of water to obtain dispersion A. Take a centrifuge tube, add 3 g of the above dispersion A, add 5 ml of 2M HCl solution and sonicate for 8 minutes, and then separate and remove the green clear liquid with a magnet. Then disperse with 10ml of water, collect the particles with a magnet, remove the light green clear liquid, disperse with 5ml of 10wt% trisodium citrate solution ultrasonically, let stand for 2h, and disperse with 5ml of...

Embodiment 2

[0033] Substantially the same as in Example 1, the microbial water purifying agent used for pufferfish culture water is obtained, the difference is only that the surface modification agent is different in the step (1-4). In Example 2, 0.3 g of dimethyloctylsilane was used as the surface modifier.

Embodiment 3

[0035] Substantially the same as in Example 1, the microbial water purifying agent used for pufferfish culture water is obtained, the difference is only that the surface modification agent is different in the step (1-4). In Example 3, 0.3 g of octadecyltriethoxysilane was used as the surface modifier.

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PUM

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Abstract

The invention discloses a microbial water purifying agent for puffer fish culture water and a preparation method thereof. The preparation method comprises the following steps: adding 30-40 parts by weight of surface-modified C8 and C18 alkyl-chain magnetic silicon balls into 300-400 parts by weight of water; adding 80-120 parts by weight of microbial reagent, including 60-80 parts of Synechococcus, 50-70 parts of Rhodobacter sphaeroides, 20-30 parts of Nitrosospira, 20-30 parts of Nitrosococcus, 10-30 parts of Pseudomonas stutzeri, 20-30 parts of iron bacteria, 3-6 parts of Thiothrix, 3-6 parts of Beggiatoa, 50-80 parts of Sarcomastigophora, 50-70 parts of amoebae, 30-50 parts of Tetraselmis and 30-40 parts of dunaliella tertiolectra; and adding 60-80 parts by weight of beef extract peptone agar culture medium, and culturing. The microbial water purifying agent can be added into a puffer fish culture tank to achieve the goal of purifying culture tank water.

Description

technical field [0001] The invention relates to a microbial water purifying agent and a preparation method thereof, in particular to a microbial water purifying agent used for pufferfish breeding water and a preparation method thereof. Background technique [0002] In recent years, the rapid advancement of high-efficiency agriculture has led to the rapid development of special aquaculture. Famous, special and high-quality new aquaculture species have reached the level of large-scale production and formed their own characteristics. Puffer, English name: Fareastpuffers, commonly known as "bubble fish", "blowing belly fish", "river puffer fish", "air drum fish" (Jiangsu, Zhejiang), "good fish" (Guangdong), "chicken hug" (Guangdong), "turtle fish" (Guangxi), "street fish" (Fujian), "wax head" (Hebei), "boat mackerel". Puffer fish are warm temperate and tropical offshore bottom fish, inhabiting the ocean In the middle and lower layers, a small number of species enter freshwater...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/325C02F3/34
Inventor 许银亚
Owner 徐州邦尼生物技术有限公司
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