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A kind of microbial water purifying agent for Chinese soft-shelled turtle breeding water and preparation method thereof

A technology for microbial reagents and aquaculture water, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve problems such as water pollution, production decline, Chinese soft-shelled turtle inadaptability, etc., and achieve easy High precipitation and purification efficiency

Active Publication Date: 2016-03-02
上海乾界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of artificial breeding, because the breeding density far exceeds the natural level, the water body cannot handle the excess bait and the excrement of the Chinese soft-shelled turtle, causing the water body to be polluted, the dissolved oxygen to drop, and the ammonia nitrogen index to rise. The growth of soft-shelled turtles is adversely affected
[0003] In the current aquaculture technology, because the aquaculture water cannot be treated, it is easy to cause problems such as an increase in the incidence of soft-shelled turtles, a decrease in survival rate, and a decrease in production.
The conventional solution is to change the water at intervals, but on the one hand, changing the water will increase the production cost, and on the other hand, it will easily cause the unsuitable symptoms of the soft-shelled soft-shelled turtle.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of magnetic silicon spheres with surface-modified C8 and C18 alkyl chains:

[0025] (1-1) Synthesis of Fe by hydrothermal method 3 o 4 Magnetic nanoparticles: 1 g of FeCl 3 ·6H 2 O, dissolved in 30ml of ethylene glycol, adding 4 grams of anhydrous sodium acetate, the reaction temperature is 200 ° C, the reaction time is 12 hours, and Fe is generated 3 o 4 magnetic nanoparticles;

[0026] (1-2) the above Fe 3 o 4 Wash the magnetic nanoparticles with water to remove water-soluble impurities, and then disperse them in 10 g of water to obtain dispersion A. Take a centrifuge tube, add 3 g of the above dispersion A, add 5 ml of 2M HCl solution and sonicate for 8 minutes, and then separate and remove the green clear liquid with a magnet. Then disperse with 10ml of water, collect the particles with a magnet, remove the light green clear liquid, disperse with 5ml of 10wt% trisodium citrate solution ultrasonically, let stand for 2h, and disperse with 5ml of...

Embodiment 2

[0033] Basically the same as in Example 1, the microbial water purifying agent used for soft-shelled soft-shelled turtle culture water was obtained, the only difference being that the surface modification agent was different in the step (1-4). In Example 2, 0.3 g of dimethyloctylsilane was used as the surface modifier.

Embodiment 3

[0035] Basically the same as in Example 1, the microbial water purifying agent used for soft-shelled soft-shelled turtle culture water was obtained, the only difference being that the surface modification agent was different in the step (1-4). In Example 3, 0.3 g of octadecyltriethoxysilane was used as the surface modifier.

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Abstract

The invention discloses a microbial water clarifying agent for Chinese softshell turtle cultivation water and a preparation method thereof. The preparation method comprises the following steps: adding 20-40 parts by weight of magnetic silica spheres with C8 and C18 alkyl chain-modified surfaces into 200-400 parts by weight of water; adding 80-100 parts by weight of microbial reagents, wherein the microbial reagents comprise the following raw materials: 50-70 parts of rhodopseudomonas palustris, 50-70 parts of oscillatoria cyanobacteria, 10-30 parts of nitrosospira, 10-30 parts of nitrosolobus, 10-20 parts of thiobacillus dentrificans, 10-20 parts of leptothrix, 1-3 parts of thiothrix, 1-3 parts of beggiatoaceae, 30-70 parts of paramecium, 30-70 parts of ciliate, 20-40 parts of platymonas subcordiformis and 20-40 parts of blue algae; and then adding 40-60 parts of by weight of beef extract peptone agar medium for culturing. The microbial water clarifying agent can achieve the purpose of purifying the cultivation water after being added into a Chinese softshell turtle culture pond.

Description

technical field [0001] The invention relates to a microbial water purifying agent and a preparation method thereof, in particular to a microbial water purifying agent for Chinese soft-shelled turtle breeding water and a preparation method thereof. Background technique [0002] In recent years, the rapid advancement of high-efficiency agriculture has led to the rapid development of special aquaculture. Famous, special and high-quality new aquaculture species have reached the level of large-scale production and formed their own characteristics. Chinese soft-shelled turtle is a high-protein (15-17%), low-fat (1-4%) aquatic product containing multiple vitamins and trace elements. It has high edible and medicinal value and high economic value. Chinese soft-shelled turtle mainly eats animal food. Under artificial breeding conditions, the body weight can reach about 400-500g after 12-15 months. Feed nutrition: protein accounts for 40-50% (animal protein accounts for more than 80%...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/325C02F3/34
Inventor 许银亚
Owner 上海乾界生物科技有限公司
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