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Screening of high-temperature-resistant and high-light-resistant Tetraselmis

A technology of four-seamed algae and cultivation method, which is applied in the field of microalgae cultivation, and can solve the problems of restricting the industrial production of microalgae and difficulty in microalgae cultivation

Active Publication Date: 2022-05-31
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Microalgae farming is more difficult in southern coastal areas
Because the summer temperature in the southern region is much higher than the suitable growth temperature of microalgae, microalgae cannot grow rapidly in such an environment, which greatly limits the industrial production of microalgae

Method used

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  • Screening of high-temperature-resistant and high-light-resistant Tetraselmis
  • Screening of high-temperature-resistant and high-light-resistant Tetraselmis
  • Screening of high-temperature-resistant and high-light-resistant Tetraselmis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The isolation and identification of embodiment 1 algae strain

[0035] (1) Isolation and cultivation of algal strains

[0036] Collect soil and water samples. After the samples arrived in the laboratory, the stones, pebbles and straws were removed to clean the samples, and the samples were kept at 4°C for use. Thoroughly mix the soil sample, and add a representative 10-15 g soil or 10 mL homogeneously mixed water sample into a flask containing 100 mL of F / 2 medium.

[0037] Place the flask in a constant temperature environment at 25°C, and give an intensity of 100μE·m -2 ·s -1 , 16 hours of light every day, until the color of the culture medium turns green or brown, indicating that algae are growing, and the algae suspension is obtained.

[0038] The algal suspension was spread on F / 2 agar plates, and after several days, an approximately uniform mixed microalgal and fungal contamination was observed. The microalgae strains with fast growth rate were selected and pu...

Embodiment 2 4

[0063] Embodiment 2 Tetraselmis sp (MEW-A-176) tolerance test:

[0064] (1) Effects of different temperatures on the growth of algal species

[0065] The algae liquid was inoculated in F / 2 medium, the initial OD 750nm The value is 0.5, respectively set the culture temperature of 15°C, 25°C, 28°C, and 35°C, and give 50μE·m -2 ·s -1 , 24 hours / day of light for cultivation, and the OD of each group of algae liquid was measured on the second day, the fourth day, the sixth day and the eighth day of the culture 750nm value.

[0066] The result is as figure 1 As shown, under four temperature conditions, the OD of Tetraselmis sp(MEW-A-176) 750nm The values ​​gradually increase with time, and within the temperature range of 28°C to 35°C, the higher the temperature, the OD of Tetraselmis sp(MEW-A-176) 750nm The larger the value, the highest value appears under the condition of 35°C.

[0067] (2) The effect of different light on the growth of algal species

[0068] The algae liqu...

Embodiment 3 4

[0073] Example 3 Tetraselmissp (MEW-A-176) Physiological Index Test Test

[0074] Algae cells are rich in nutrients, including proteins, carbohydrates, lipids, etc. Proteins are important components and functional molecules that make up cells. Many physiological functions, including cell survival, need to be realized through proteins. Carbohydrates are the main components of the structure of living cells and the main energy supply substances, and have the function of regulating cell activities. Starch is the main storage form of sugar in plants, and its content determination is of great significance for evaluating the nutritional value of food and sugar metabolism in plants. Lipids are the most important form of energy storage for organisms. The lipids of microalgae are mainly divided into two categories: neutral lipids and polar lipids. The neutral lipids are mainly composed of triglycerides, sterol lipids, and free fatty acids. , while polar lipids are mainly composed of p...

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Abstract

The invention relates to the technical field of microalgae culture, in particular to screening of high-temperature-resistant and high-light-resistant Tetraselmis. The Tetraselmis is obtained through screening, the number is CCTCC NO: M20211216, the OD750 cultured at the high temperature of 35 DEG C can reach 3.0 or above, and the growth vigor of the Tetraselmis under the illumination condition of 200 [mu] E.m <-2 >. S <-1 > is better than that under the illumination condition of 50 [mu] E.m <-2 >. S <-1 >. Under high-temperature and high-light culture conditions, the algal strain is high in density, the dry weight of the algal strain contains 48.156% of protein (including abundant essential amino acids), 24.57% of carbohydrate, 17.109% of starch and 12.499% of grease, and polyunsaturated fatty acid accounts for 62.3% of total fatty acid. The algal strain is rich in nutrition and suitable for being used as bait for fishes and shrimps.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to the screening of a high-temperature and high-light-resistant Tetraphyllum algae. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in water bodies, rich in nutrients and high in photosynthetic utilization. They can use light energy and absorb CO through their efficient photosynthesis. 2 and H2O, converting them into organic matter such as carbohydrates, proteins, fats, and releasing O 2 . Cell metabolism produces polysaccharides, proteins, oils, pigments, etc., which have broad development prospects in the fields of pharmaceutical industry, food industry, and biological bait. [0003] Microalgae itself is a pure natural green biological bait, which has a high content of essential amino acids and is rich in polyunsaturated fatty acids. Microalgae are rich in nutrients and are easily absorbed and digested by fish and shri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A23K10/16A23K50/80C12R1/89
CPCC12N1/12A23K50/80A23K10/16Y02A40/81
Inventor 路延笃杨琳
Owner HAINAN UNIVERSITY
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