Experimental method of effective degradation of nonylphenol by microalgae

An experimental method, the technology of nonylphenol, is applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., and can solve problems such as high cost and complicated methods

Active Publication Date: 2019-01-18
JINAN UNIVERSITY
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Problems solved by technology

[0003] However, these methods have disadvanta

Method used

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Embodiment

[0028] After nonylphenol was exposed to four kinds of microalgae, 0.4% methanol co-solvent was added to four kinds of microalgae, and the effect of environmental hormone nonylphenol on four kinds of microalgae was characterized according to the change of chlorophyll a concentration of four kinds of microalgae, four kinds of microalgae The chlorophyll a content of the microalgae was compared with the control group without adding NP or methanol and only microalgae existed, adding 0.4% methanol co-solvent had no effect on the growth of microalgae. The growth of the four microalgae showed a significant time-dose effect. When the concentration of nonylphenol was 0.5 mg / L, Nannochloropsis and subcardioid algae exhibited growth-promoting phenomena at low concentrations, while Phaeocystis globosa and Dunaliella salina had no significant difference from the control group, both With the increase of culture time, the content of chlorophyll a also increased. In the concentration treatmen...

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Abstract

The invention discloses an experimental method of effective degradation of nonylphenol by microalgae. The experimental steps are as follows: 1) cultivation of four kinds of sterile microalgae; 2) extract and measuring chlorophyll a; 3) set up four groups of nonylphenol experiment group; 4) The correlation of microalgae absorbance with cell density and biomass in the degradation of nonylphenol by microalgae; 5) The removal of nonylphenol by microalgae and the extracellular adsorption and intracellular absorption of nonylphenol by microalgae. The four marine microalgae screened out by the invention have the functions of absorption, adsorption and degradation of nonylphenol. They were Phaeocystis globosa, Pseudochlorococcus sp., Dunaliella salina and Platymonas subcordiformis. The removal rates of nonylphenol (including adsorption rate, absorption rate and degradation rate) by the four microalgae ranged from 47.18% to 59.05%. The removal rates of nonylphenol by Phaeocystis globosa, Pseudochlorococcus sp., Dunaliella salina and Platymonas subcordiformis were higher than that by P. 96.36%, the degradation rate was 43.43%-90.94%. Among the four microalgae, Platymonas subcordiformis had the most significant removal and degradation of NP.

Description

technical field [0001] The invention belongs to the experimental field of degrading nonylphenol, and more specifically relates to an experimental method for effectively degrading nonylphenol by microalgae. Background technique [0002] Since nonylphenol has many serious potential hazards to the ecosystem, the degradation and removal of p-nonylphenol has become a research hotspot. The method of nonylphenol degradation mainly includes physical methods (such as activated carbon, etc.). Sasai et al. found that when organic montmorillonite made of HDTMA-modified montmorillonite (filtered through a 0.20mm membrane filter) adsorbed NP, within 10min, 1mg The organic montmorillonite can adsorb about 0.1mg of NP molecules, and its adsorption rate for NP molecules is almost 100%. is a problem. The chemical method (ultrasonic chemical oxidation method, etc.) uses ultrasonic frequency of 200kHz and the intensity is 6W cm-2. When using ultrasonic waves to degrade 30μM NPs, it takes 100m...

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12R1/89
CPCC12Q1/02G01N2333/405
Inventor 段舜山王璐畇孙东朱博陈琪
Owner JINAN UNIVERSITY
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