A kind of rapid propagation method for the rejuvenation of Lvfengteng tea variety
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A technology of phoenix vine and varieties, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that viruses cannot be guaranteed and are difficult to survive, and achieve clustered buds and buds with high value-added coefficients, easy to master, and methods simple effect
Active Publication Date: 2016-04-20
LAIFENG FENGYA TENGCHA BIOLOGICAL
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Problems solved by technology
The smaller the shoot tip, the better, but it is not easy to survive if it is too small, and the virus cannot be completely removed if it is too large
Method used
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Embodiment 1
[0023] A kind of tissue culture rapid propagation method of Lvfengteng tea variety rejuvenation, its steps are:
[0024] 1) Selection and sterilization of explants:
[0025] Get the stem tips of 100 healthy young shoots of Lvfengteng tea, and use them for future use after surface sterilization.
[0026] Select 100 newly germinated shoots of 10 or 13 or 15 cm, cut off the leaves and tendrils, cut into 0.5 cm long stems with terminal buds, soak them in detergent for 5 or 8 or 10 minutes, then gently scrub them with a brush, Rinse with running water for about 3 hours, move to the ultra-clean workbench, wash with 70% (volume ratio) alcohol for about 30 seconds, wash with aseptic water for 3 or 4 or 5 times, and then put it into the mixture of mass (g) and volume (mL). Shock disinfection in 0.1% mercuric chloride solution for 7 minutes, rinsed with sterile water for 5 times, cut off 0.5mm shoot tips under a dissecting microscope, and inoculated them on the primary culture medium; ...
Embodiment 2
[0040] A kind of tissue culture rapid propagation method of Lvfengteng tea variety rejuvenation, its steps are:
[0041] 1) Selection and sterilization of explants:
[0042] Get the stem tips of 100 healthy young shoots of Lvfengteng tea, and use them for future use after surface sterilization.
[0043] Select 100 newly germinated shoots of 10 or 12 or 14 or 15 cm, cut off the leaves and tendrils, cut into 0.5 cm long stems with terminal buds, soak them in detergent for 5 or 7 or 10 minutes, and then gently scrub with a brush Clean it, rinse it with running water for about 3 hours, move it to the ultra-clean workbench, wash it with 70% alcohol for about 30 seconds, wash it with sterile water for 3 or 4 or 5 times, and then put it in 0.1% mercuric chloride solution for 7 minutes. After washing with bacterial water for 5 times, cut off the shoot tip of 0.5 mm under the dissecting microscope, and inoculate it on the primary culture medium;
[0044] 2) Preparation of culture med...
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Abstract
The invention discloses a rapid propagation method for the rejuvenation of the variety of green wind ampelopsis grossedentata. The method comprises steps of (A) selecting an explant and sterilizing, namely taking the stem tip of a tender branch of the green wind ampelopsis grossedentata, sterilizing the surface and reversing for later use; (B) primarily cultivating the sterilized stem tip of the tender branch of the green wind ampelopsis grossedentata, namely inoculating the explant subjected to the surface sterilization into a primary culture medium under an aseptic condition, and transferring into a culture medium for inducing and differentiating clustered buds; (C) enrichment cultivating, namely cutting the clustered buds into single plants under the aseptic condition and inoculating into a enrichment culture medium for enrichment culture; (D) strengthening seedlings; (E) rooting, namely cutting the clustered seedlings cultivated through strong seedling culture into single plants, inoculating into a rooting culture medium for rooting culture; (F) transplanting the rooting tissue culture seedlings into a medium, cultivating for 1-2 months so as to obtain mature seedlings. The method is easy, is simple and convenient to operate, effectively prevents the inoculating contamination rate of the explant, has the inoculating success rate being over 85%, is capable of growing regular roots and cultivating the strong seedlings, has the high survival rate, and is applicable to commercial production of the green wind ampelopsis grossedentata seedlings.
Description
technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation for plant stem tip detoxification to achieve variety rejuvenation, and more specifically relates to a method for tissue culture rapid propagation of Lvfengteng tea variety rejuvenation. Background technique [0002] Vine tea is a woody vine (A. grossedentata (Hand.-Mazz.) W.T.Wang) of the genus Ampe|opsisMichaux of the family Vitaceae (Vitaceae), which is rich in flavonoids (dihydromyrica rubra Factors), amino acids, polysaccharides, polyphenols, vitamins and other active ingredients. Modern pharmacology and biological activity studies have shown that vine tea has various biological activities such as anti-tumor, immune regulation, anti-oxidation, hypoglycemic, and hypolipidemic. Lvfengteng tea (A. grossedentata CV, lufeng,) is a clone selected and bred by Zheng Xiaojiang and others for many years. It is registered as a popular variety by the Variety Approval Committee, ...
Claims
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