Golden leaf carex shoot-tip culture quick propagation method

Abstract

The invention relates to a rapid culture and propagation method of Carex evergold stem tips, which belongs to the technology field of the plant propagation, including the following steps: preparing the culture medium, selecting and sterilizing the explants, induction culturing, proliferation culturing, rooting culturing and transplanting. The method has the advantages that the detergent soaking pretreatment and the two-step sterilization of the explants effectively prevent the explant inoculation from pollution and the inoculation rate reaches 85%; the optimal culturing medium of the provided Carex evergold tissue culturing is pertinent and applicable. The induction rate of the buds reaches more than 90% and the bud is more than five times the proliferation rate in each culture period. When inoculating in proliferation culturing, breaking off the multiple shoots into simple buds instead of cutting by the scalpel, the rooting portion is prevented from forming the calli, the differentiation of all-green or all-white tissue culturing is reduced, and a yield rate of more than 95% is reached. The growth rate of the buds is high with the rooting rate at 100% and the transplanting survival rate at more than 90%, which is applicable to the factory mass production.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a method for rapid propagation of Carex aureus shoot tip culture. Background technique [0002] Carex evergold (Carex evergold) belongs to Cyperaceae Carex genus, perennial herb, soft and elegant, plant height 20 cm, leaves with stripes, green edges on both sides, yellow in the middle, spikes, flowering period from April to May, It is light-loving, semi-shade-tolerant, not water-logging resistant, and has strong adaptability. It can be used as a foliage plant for flower beds and flower borders, or as a potted ornamental plant. It is conducive to the construction of low-cost garden landscapes. Its garden ornamental value has attracted the attention of industry insiders. Pay attention to. At present, Carex aureus is generally propagated by division, the propagation speed is relatively slow, and the seed germination rate is low. [0003] Zhejiang Aca...

AI Technical Summary

Problems solved by technology

However, different plants use plant tissue culture methods to propagate seedlings with different degrees of difficulty. In the early tissue culture test of Carex aureus, it was found that the growth points of Carex aureus are mostly close to the soil or even buried in the soil, which infects various microorganisms in the soil, etc. There are many opportunities for disease, and sterilization treatment is more difficult; problems such as unstable golden leaf traits of tissue culture seedlings of Phnom Penh plants or golden heart plants also occur when tissue culture seedlings are multiplied and cultivated, making it impossible to realize large-scale industrial production