Supercharge Your Innovation With Domain-Expert AI Agents!

Pregestational gene warning diagnostic kit

A kit and gene technology, applied in the pre-pregnancy gene alarm diagnostic kit and its detection field, can solve the problems of side effects of embryo growth and development, failure to reach early warning/diagnosis of neural tube defects, etc., and achieve mature methods, stable and reliable results, and easy operation easy effect

Inactive Publication Date: 2004-06-30
SICHUAN UCAN BIO TECH
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the purpose of pre-pregnancy early warning / diagnosis of neural tube defects cannot be achieved; moreover, the above methods have certain side effects on embryo growth and development, so it is necessary to seek an earlier, safer and more sensitive detection method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pregestational gene warning diagnostic kit
  • Pregestational gene warning diagnostic kit
  • Pregestational gene warning diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0046] The following are embodiments of the present invention:

[0047] The DNA extraction solution in accompanying drawing 1 is made up of erythrocyte lysate and polymerase chain reaction (PCR) buffer / nonionic detergent / proteinase K. Among them, the erythrocyte lysate is sterilized triple distilled water, and the polymerase chain reaction (PCR) buffer / nonionic detergent / proteinase K is composed of trishydroxymethylaminomethane (Tris), magnesium chloride (MgCl 2 ), TritonX-100 (TritonX-100), Tween-20 (Tween-20), wetting agent p-40 (NP-40) and proteinase K (proteinase K); other ingredients are polymerized Enzyme Chain Reaction (PCR) Mixture, Digestion Mixture, DNA Polymerase (Taq Enzyme) Solution and Restriction Endonuclease Solution. Wherein, the polymerase chain reaction (PCR) mixture includes N 5 , N 10 -Methylenetetrahydrofolate reductase polymerase chain reaction (PCR) mixture, methionine synthase reductase polymerase chain reaction (PCR) mixture, respectively by deioni...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a pregestational gene alarm diagnosis kit and its detection method, including DNA extraction liquor for extracting DNA template of testee, PCR mixed liquor for amplifying specific fragment on the genomic DNA of testee, DNA polymerase, PCR product enzyme excision mixed liquor, restriction endonuclease, positive control template of homozygous mutation and coupling pipe for making PCR reaction. The described alarm gene includes N5, N10-methylene tetrahydrofolate reductase and methionine synthetase reductase gene, their mulant sites are respectively 667-cytosine-thymine and 66 adenine-guanine, and their primer sequences respectively are 5'-TGA AGG AGA AGG TGT CTG CGG GA-3', 5'-AGG ACG GTG CGG TGA GAG TG-3', 5'-GCA AAG GCC ATC GCA GAA GAC AT-3' and 5'-GTG AAG ATC TGC AGA AAA TCC ATG TA-3'. It can be used for pregestational diagnosis of that filial generation produces neural tube deform or not.

Description

technical field [0001] The invention relates to a gene alarm kit and a detection method for predicting / diagnosing whether neural tube defects (neurae tube defects, NTDs) occur in offspring by detecting multiple gene mutation sites of both parents before pregnancy. Background technique [0002] Neural tube defects (NTDs) are a group of severe birth defects mainly including anencephaly and spina bifida, and are the birth defects with the most frequent incidence and the most serious clinical consequences in babies born in my country and the world. one. The incidence rate of neural tube defects in my country is 3.0‰, and the incidence rate of neural tube defects in some northern regions is as high as 10-20‰, which is currently known as the region with the highest incidence of neural tube defects in the world. At least 100,000 children with neural tube defects are born in my country every year (accounting for 1 / 4 to 1 / 3 of the total number of children with neural tube defects in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12Q1/6851C12Q1/6883
CPCC12Q1/6883C12Q1/6851
Inventor 李勇李竹赵如冰杨阳
Owner SICHUAN UCAN BIO TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com