31 results about "Autotransplantation" patented technology

The invention discloses a preparation technology of a novel nerve conduit, and belongs to the technical field of tents of biological tissue engineering. The preparation technology sequentially comprises a preparation technology of GDNF (glial-derived neurotrophic factor)-carried gelatin microspheres, a synthesizing technology of gelatin-methacrylamide hydrogel, a preparation method of polycaprolactone/gelatin electricity texture nerve conduits, and a combining method of the electricity texture nerve conduits and the GDNF-carried gelatin microspheres. The preparation technology has the advantages that a tent structure with good degradability, biological compatibility and biological safety is realized, and the GDNF is controlled and released by the GDNF-carried gelatin microspheres; compared with blank groups and collagen conduit groups, the regeneration of peripheral nerves is effectively promoted, and the structure and function of restoring damaged nerves can reach the effect similar to the effect of autotransplantation groups; the defect nerves can be bridged, and the stability of axon regeneration environment can be maintained; the invasion of peripheral connective tissues is prevented; the saturing of near and far-heart breaking ends of smaller nerve defect is solved, and the restoration of larger nerve defect is solved.

The invention provides a method for enriching and extracting adult stem cells from inside of human being or animals, namely, a physiologically acceptable multihole tri-dimensional tissue engineering material is implanted inside human being or animals to enable the adult stem cells to be enriched on the material, and then the adult stem cells are separated from the materials, purified and proliferated to realize autotransplantation.

The invention relates to the field of the biotechnology, in particular to an adipogenesis induction culture medium and an adipogenic differentiation method. The adipogenesis induction culture medium comprises 3-isobutyl-1-methylxanthine, insulin, indomethacin, dexamethasone, rosiglitazone, PRP (Platelet Rich Plasma) and a basic culture medium. The invention provides an adipogenic differentiation method which enables various inducing factors to be subjected to combined application to act on mesenchymal stem cells including GMSCs and like, has a synergistic effect and can effectively induce the adipogenic differentiation of the mesenchymal stem cells, and the adipogenic differentiation effect of the adipogenic differentiation method is obviously superior to that of an existing conventional induction method. The GMSCs autologous materials are abundant, are easy in expansion in vitro and can be used for autotransplantation so as to avoid immunological rejection reaction.

The invention discloses an extracting and in-vitro multiplication culture technical method of urine-derived mesenchymal stem cells (USCs). In-vitro multiplication is mainly conducted in the mode thatthe mesenchymal stem cells (MSCs) are separated from sterile urine, and a culture medium easy to prepare is adopted; the extracted USCs subjected to multiplication culture can be used for therapy of clinical immunology related diseases and damage repair of various tissue and organs; the extracting and in-vitro multiplication culture technical method has the advantages that the separating and extracting processes of the USCs are completely free of wounds, special instruments are not needed, the cost is low, and the extracting process is easy to accept by patients; the USCs have other advantagesof being capable of achieving autotransplantation, free of immune rejection response and ethical arguments, and capable of avoiding the pathophoresis risk; and the USCs show the good multiplication ability, and the number of the cells needed during cell therapy can be met.

The invention belongs to the technical field of regenerative medicine and discloses a method for preparing an adipose-derived ECM (extracellular matrix) through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, manure adipose cells and SVF cells are removed, the ECM is prepared, and too low ECM content and cell factor loss caused by complex operation are avoided effectively. According to the method, the ECM rich in cell factors is prepared without addition of exogenous chemical substances, the prepared ECM contains no exogenous substances, and adipose autotransplantation and effectiveness of the ECM as a biological filling material can be improved. The adipose-derived ECM material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The invention discloses an electrical stimulation device for promoting autotransplantation fat flap regeneration. The electrical stimulation device comprises a keyboard control module, a micro-controlmodule, a current controlled bipolar power module, a digital voltmeter head, a sampling module, an electrode A and an electrode B. The electrical stimulation device provided by the invention adopts amodular design, and is more flexible and convenient to use, convenient in device maintenance, low in cost, multiple in output waveform catalog, controllable in various parameters, and good in treatment effect.

The invention provides a culture medium for separating and inducing human adipose stem cells into pancreatic beta cells and a use method of the culture medium. The optimal culture medium is designed; gene transfection is not required, so that the risk of gene modification and cancers is avoided, few induction steps are used, the use is simple, and the induction time is short and is shortened by 30% in comparison with the prior art; the culture medium is safe, nontoxic and high in success rate of induction. Moreover, after autogenous adipose mesenchymal stem cells are induced and differentiated into insulin-secreting cells, rejection and ethical problems are avoided after autotransplantation, and the safety is high, so that the clinical application prospect is wide. The culture medium is simple, feasible, low in cost and good in use effect.

The invention belongs to the technical field of regenerative medicine and discloses a preparation method for efficiently enriching a SVF (stromal vascular fraction) cell material through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, and meanwhile, oil drops produced by crushed mature adipose cells are collected with a floccus centrifugal precipitation technology, so that the effect of enriching SVFs is realized. After ultrasonic treatment, the cell viability is substantially improved and the cell apoptosis rate is substantially decreased, so that the transplanting effectiveness is greatly enhanced. The cell material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The present invention provides a medical composition which, in autotransplantation which is the only method that can induce the epithelialization even in a widespread full-thickness skin defect, enables the fixation of an autotransplantation skin graft in a simple manner and can increase the efficiency of epithelialization to promote the regeneration of the skin. The present invention elates to a medical composition comprising a photo-crosslinkable chitosan derivative and an amino acid and/or a saccharide. The amino acid is preferably an essential amino acid, and the saccharide is preferably a neutral saccharide selected from glucose, galactose, mannose and fucose.

The invention discloses a method and product for repairing the HBB gene of a hematopoietic stem cell. According to the method, the technology that missed site codon 41/42(-TCTT) in beta-thalassemia (thalassemia) is subjected to targeted knockout by utilizing a CRISPR-Cas9 gene editing technology; sgRNA capable of identifying and guiding Cas9 protein to the target sequence of a target gene is designed and synthesized; and the sgRNA and Cas9 protein are mixed and electrically transfected into the hematopoietic stem cell of the beta-thalassemia codon 41/42 (-TCTT), meanwhile, a homologous recombination donor is introduced to efficiently repair the normal coding function of amino acid at the mutation site and recover the normal expression of the beta-thalassemia gene. The transfusion dependent type beta-thalassemia codon 41/42 (-TCTT) is edited by utilizing the existing gene editing technology which has high repairing efficiency, the repaired hematopoietic stem cells of the patient can reconstruct the blood system of the patient and treat thalassemia after autotransplantation.

The invention discloses a method for evaluating the blood supply state of parathyroid glands in an operation, which comprises the following steps of: in a thyroid operation, identifying and confirming parathyroid glands, fixing the parathyroid glands by using tweezers, taking out a needle head to puncture the envelop of the parathyroid glands, puncturing the parathyroid glands into the glands, pulling out the needle head, and observing the bleeding condition on a needle eye on the parathyroid glands. The blood supply state of the parathyroid gland is evaluated according to the grading of the bleeding condition, and whether the parathyroid gland is suitable for in-situ retention or autotransplantation is determined. The blood supply condition is determined by directly puncturing the parathyroid gland, the method is safe, accurate and reliable, the operation is simple and convenient, time is saved, meanwhile, special equipment is not needed, and the method can be widely developed and applied clinically; the syringe needle with the size can ensure that the blood supply state of the parathyroid gland can be accurately evaluated, massive hemorrhage of the parathyroid gland cannot be caused, and the syringe needle is safe and efficient.

The invention relates to the field of cells, in particular to a composition, an inducing preparation containing the composition and an inducing method. The composition is prepared from 5-azacytidine, bone morphogenetic protein and angiotensin II. The invention further provides an inducing preparation. In the inducing preparation, the final concentration of 5-azacytidine is 5-15 micromole/L, the final concentration of the bone morphogenetic protein is 5-15 mcg/L, and the final concentration of the angiotensin II is 1-10 micromole/L. The invention provides the method for effectively inducing GMSCs into cardiomyocyte. A DMEM/F12 culture medium with the culture solution components comprising 10% of FBS, 10 micromole/L of 5-aza, 10 ng/mL of BMP-2 and 5 micromole/L of Ang-II is subjected to induction differentiation. By means of the method, the GMSCs can be effectively induced to cardiomyocytes differentiation. The materials of the GMSCs are convenient to obtain, in-vitro expansion is easy, the GMSCs can be used for autotransplantation, and therefore an immunological rejection reaction is avoided.

The invention discloses a method and application for separating, culturing and amplifying cells, in particular relating to a method for preparing monocyte type stem cells and application thereof in preparing medicaments. In the invention, the monocyte type stem cell is obtained by separating a monocyte from human blood, culturing and amplifying the monocyte in a culture medium containing cell growth factors and separating the monocyte from the culture medium. The invention has the advantages of wide source of the use materials, favorable use effect and is safer and more reliable in the treating process, and an autotransplantation can be realized. The invention has wide use for the medical development and the disease treatment.

The invention discloses a preparation method of menstrual blood mesenchymal stem cells, the cell source is novel adult stem cells separated and cultured from female menstrual blood, the cell source is rich, the separation and culture process is safe and simple, the menstrual blood mesenchymal stem cells can be massively researched and used, the stem cells separated and cultured from female menstrual blood have all the characteristics of mesenchymal stem cells and are extremely easy to proliferate, meanwhile, the menstrual blood mesenchymal stem cells can be induced to be differentiated into various tissue cells including nerve cells, adipose cells, islet cells, ovarian cells and the like and can also be induced into multifunctional stem cells, the research value is high, the menstrual blood mesenchymal stem cells are high in self-renewal capacity, easy to obtain and stable in in-vitro culture, autotransplantation can reduce mental and psychological pressure of patients, the clinical application prospect of the menstrual blood mesenchymal stem cells is far better than that of bone marrow mesenchymal stem cells, chromosome karyotype has no abnormal change after multiple passages, and the safety of the menstrual blood stem cells in the clinical application field is indicated.

On a theoretical basis that ultraviolet light (UV) and cobalt chloride simulate low oxygen to induce cell apoptosis, the research uses CoCl2 to simulate a low oxygen environment and uses 280 nm LED UVto irradiate an acute promyelocytic leukemia cell (HL-60 cell), probes into proliferation situation of the HL-60 cell after the HL-60 cell is irradiated by the 280 nm LED UV under the low oxygen environment, discovers that both the 280 nm LED UV and low oxygen inhibit proliferation of the HL-60 cell and induce the cell apoptosis, and the 280 nm LED UV has more obvious proliferation inhibition andapoptosis effects on the cell under the low oxygen condition compared with a normoxic condition. Through irradiating a transplant by the 280 nm LED UV under the low oxygen condition, remaining tumorcells in the transplant are led to apoptosis and necrosis, normal cells can survive normally, so that the ultimate purpose of eliminating remaining tumors is achieved, recurrence rate of leukemia patients after autotransplantation is reduced, and application prospect of the 280 nm LED UV in leukemia autotransplantation purging in vitro is indicated.

The invention discloses a preparation method and application of a high-conductivity healing-promoting multichannel nerve conduit based on a 3D printing technology, the nerve conduit comprises raw materials of polylactide-poly (trimethylene carbonate) and trititanium dicarbide, and also comprises solvents of N, N-dimethylformamide and dichloromethane, the prepared MXPLT conduit has quite high ductility, and the healing-promoting multichannel nerve conduit is suitable for being used in the field of medical instruments, such as medical instruments, medical instruments, medical instruments, medical instruments, medical instruments, medical instruments, medical instruments, medical instruments, medical instruments and medical instruments. The material can deal with catheter deformation caused by muscle human body movement in the nerve repairing process and is suitable for being used as a nerve repairing material for repairing nerve broken ends. The MXPLT catheter is more beneficial to electrical signal conduction, and is more beneficial to repairing of a nerve broken end, meanwhile, the MXPLT catheter has excellent shape memory ability and can recover to an initial shape within a short time when deformation is generated due to the influence of external force in the repairing process, the repairing effect of the MXPLT catheter group is closest to that of autotransplantation repairing, and the MXPLT catheter group has an excellent repairing effect.

The invention relates to the technical field of biomedicine, in particular to a method for obtaining mesenchymal stem cells from mouse gingival tissue. The method comprises the following steps: collecting gingival tissues of a mouse, and digesting the gingival tissues of the mouse by adopting collagenase to obtain the mesenchymal stem cells. The mouse GMSCs obtained by the invention highly express CD29, CD44, CD73, CD90 and CD105, and hardly express CD34 and CD45. After the mouse GMSCs are cultured for 21 days by using the adipogenesis induction liquid, the mouse GMSCs are differentiated into adipocytes and secrete lipid droplets; after the GMSCs are cultured by an osteogenesis induction solution, the cells gradually deform from a long spindle to a polygon and grow in a multi-layer covering manner, small calcification appears successively, and a new research method is provided for autotransplantation of the GMSCs in animal models of inflammation, autoimmune diseases, tissue repair and the like.

The invention discloses a method for cultivating black pearls by autotransplantation of pinctada palustris, which comprises the following steps: selecting pinctada palustris, performing anesthesia treatment on the pinctada palustris before an operation, performing a nucleus implantation operation, recuperating the pinctada palustris, cultivating and managing the pinctada palustris and the like, and comprises the following steps: selecting healthy pinctada palustris with a pearl layer with strong luster on the inner surface of the pinctada palustris as the nucleus implantation pinctada palustris; the method comprises the following steps of: anesthetizing the pearl shells before a nucleus implanting operation, taking down a mantle small piece by using a special tool, implanting the taken-down mantle small piece and pearl nucleuses into connective tissues of the pearl shells with the small pieces taken, and then recuperating the pearl shells after the operation in a fish pond and cultivating the pearl shells in a pearl breeding sea area in sequence. By adopting the method to cultivate black pearls through nucleus implantation of the pinctada shells, the survival rate of the pinctada shells is high, the nucleus-remaining pearl-forming rate is high, the nacre secretion speed is high, the pearl luster is strong, the method is suitable for artificial pearl cultivation production of the pinctada shells, the method is easy to popularize and apply in pearl production enterprises and farmers, and the social benefit and the economic benefit are good.

The invention belongs to the technical field of culture of stem cells, and specifically relates to a limbal stem cell (LSC) culture medium and a culture method. In the prior art, LSCs in-vitro culturemethods encounter with situations that LSCc amplification capacity is exhausted and LSCs are spontaneously differentiated without exception, so that LSCs cells meeting transplanting requirements cannot be acquired. In allusion to a technical problem, the invention provides a culture method capable of meeting autotransplantation cell number requirements. It is indicated, by research, that throughadding SPARC (secreted protein acidic and rich in cysteine) with certain concentration into the LSCs cell culture medium, amplification capacity exhaust in an in-vitro passage process can be effectively delayed; colony forming efficiency is increased; proliferative activity is increased; a stem cell number meeting the transplanting requirements is easily acquired; and a transplanting success rateis improved.

The invention discloses an intron abnormal splicing repair method. The method is a technology for targeted knockout of an abnormal mutation site IVS2-654 C> T in beta-thalassemia (thalassemia) by using a CRISPR-Cas9 gene editing technology, and comprises the following steps: designing and synthesizing a guide RNA sequence (sgRNA) capable of identifying and guiding a Cas9 protein to a target sequence of a target gene; And electrically transferring and introducing an sgRNA and Cas9 protein mixture into beta-thalassemia IVS2-654 C>T hematopoietic stem cells to efficiently destroy an abnormal splicing mutation site, so that normal shearing and expression of a beta-globin gene are recovered. The blood transfusion dependent beta-thalassemia IVS2-654 C>T can be edited by utilizing the existing gene editing technology, the editing efficiency is high, and the edited hematopoietic stem cells of the patient can reconstruct a blood system of the patient and treat thalassemia diseases after autotransplantation.

The present invention relates to a method of obtaining high purity stem cells from tissue, comprising: providing an impurity-containing cell mass obtained from a tissue; providing a filter device which comprises a cylinder structure, wherein the cylinder structure comprise an inlet and an outlet below and a content configured inside the cylinder structure between the inlet and the outlet; culturing the impurity-free cell mass on a polymeric film, wherein target stem cells of the impurity-free cell mass conjugate into a spheroid cell population; collecting the spheroid cell population from the polymeric film to obtain high purity target stem cells. According to the method of the present invention, stem cells can be rapidly and easily obtained from tissue. Only a small amount of tissue sample is required and the stem cells obtained can be readily used in clinical applications such as autotransplantation without the requirement of in vitro amplification.