Preparation method of menstrual blood mesenchymal stem cells

A technology of mesenchymal stem cells and menstrual blood, which is applied in the field of menstrual blood mesenchymal stem cell preparation, can solve problems such as immature menstrual blood mesenchymal stem cell culture system and preparation system, virus and mycoplasma contamination, and clinical application threats, so as to reduce mental and Effects of psychological stress, easy expansion, and strong self-renewal ability

Inactive Publication Date: 2021-10-29
郑州贝因生物科技有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the above-mentioned menstrual blood mesenchymal stem cell culture system and preparation system are immature, and there may be pot

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  • Preparation method of menstrual blood mesenchymal stem cells

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Embodiment Construction

[0028] The present invention is described in further detail now in conjunction with accompanying drawing. The accompanying drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, so that they only show the components relevant to the present invention.

[0029] 11. Please refer to figure 1 , a method for preparing menstrual blood mesenchymal stem cells, comprising the following steps:

[0030] Step 1. Collect menstrual blood samples from healthy menstrual women, and collect the blood into a menstrual blood collection tube containing antibiotic preservation solution. The menstrual blood samples collected from healthy menstrual women are fresh blood. In order to maintain cell activity, they cannot be frozen or refrigerated, and the blood samples should be collected within 2 hours. When collecting menstrual blood samples, menstrual cups should be used with the volunteer donor’s knowledge and consent, a...

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Abstract

The invention discloses a preparation method of menstrual blood mesenchymal stem cells, the cell source is novel adult stem cells separated and cultured from female menstrual blood, the cell source is rich, the separation and culture process is safe and simple, the menstrual blood mesenchymal stem cells can be massively researched and used, the stem cells separated and cultured from female menstrual blood have all the characteristics of mesenchymal stem cells and are extremely easy to proliferate, meanwhile, the menstrual blood mesenchymal stem cells can be induced to be differentiated into various tissue cells including nerve cells, adipose cells, islet cells, ovarian cells and the like and can also be induced into multifunctional stem cells, the research value is high, the menstrual blood mesenchymal stem cells are high in self-renewal capacity, easy to obtain and stable in in-vitro culture, autotransplantation can reduce mental and psychological pressure of patients, the clinical application prospect of the menstrual blood mesenchymal stem cells is far better than that of bone marrow mesenchymal stem cells, chromosome karyotype has no abnormal change after multiple passages, and the safety of the menstrual blood stem cells in the clinical application field is indicated.

Description

technical field [0001] The invention relates to the technical field of stem cell preparation, in particular to a method for preparing menstrual blood mesenchymal stem cells. Background technique [0002] Menstrual blood stem cells are derived from the endometrial tissue that falls off with menstrual blood during women's menstruation. They belong to a type of adult stem cells and have a multi-directional differentiation potential close to embryonic stem cells. It has broad prospects for clinical application in major diseases such as diseases. Studies have found that stem cells derived from menstrual blood have incomparable advantages over stem cells such as embryos, bone marrow, and male blood. The number of stem cells is 30 times that of bone marrow, which can provide a rich source of stem cells for disease treatment; the ability to proliferate in vitro is strong, generally doubled in 24-36 hours, and can be passaged up to 50 times; the proliferation ability is more than 10...

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2509/00C12N2509/10
Inventor 赵辉高萧芳崔兰云段书娇刘利
Owner 郑州贝因生物科技有限公司
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