194 results about "Pulpa dentis" patented technology

Apparatus and methods for endodontic treatment of teeth provide effective cleaning of organic material (such as pulp and diseased tissue) from the root canal system. In an embodiment, a compressor system generates high pressure liquid (e.g., water) that flows through an orifice to produce a high-velocity collimated jet of liquid. The high-velocity jet is directed toward a surface of a tooth, for example, an exposed dentinal surface, and impingement of the jet onto the surface generates an acoustic wave that propagates throughout the tooth. The acoustic wave effectively detaches organic material from dentinal surfaces and tubules. The detached organic material is flushed from the root canal system by the liquid jet and / or by additional irrigation.

The present invention provides a culture of isolated adult human dental pulp stem cells that can differentiate into dentin / pulp tissue that can be used to produce a tooth in a human being. The present invention further provides a method of regenerating human dentin / pulp tissue.

Systems and methods provide for automated, cyclic delivery and evacuation of a treatment or irrigation solution from a tooth root pulp chamber and pulp canals. A manifold has a base member sized and configured to rest on a crown of an instrumented tooth and a top member sized and configured to couple with the base member to define an inlet chamber and an outlet chamber. The distal end of a needle is passed through an opening between the inlet and outlet chambers and extended distally beyond the base member into a pulp canal. The proximal end of the needle includes an opening in fluid communication with the inlet chamber. A solution is transferred from a fluid supply source to the inlet chamber and through the needle into the pulp chamber and pulp canals. The spent solution is evacuated from the pulp chamber and pulp canals through the outlet chamber.

Enhanced radiographic detection is provided by an endodontic marking instrument having a highly radiopaque elongate member, thereby enabling a dentist to better identify the location of the instrument in a root canal and the length of the root canal. The highly radiopaque, high contrast material of the elongate member is a non-toxic, material such as gold, platinum, palladium, silver, tungsten, and the like. The endodontic marking tools of the present invention are distinctly visible on radiographic images in light of the substantial contrast between the highly radiopaque material and the tooth of the patient.

A dental composition compatible with acidic dental primers / adhesives, the dental composition comprising (A) 5% (w / w) to 90% (w / w) of at least one polymerizable monomer having at least one ethylenically unsaturated vinyl, acrylate, or methacrylate group; (B) 0.01% (w / w) to 10% (w / w) of at least one compound of structurewhere each of R1, R2, and R3 may be the same or different and is independently selected from H, alkyl, alkoxyl, aryl, acyl, allyl, pyridyl, —OH, alkenyl, aralkyl, cycloalkyl, and heterocyclic structure containing O, S, or N; (C) 0.01% (w / w) to 10% (w / w) of at least one hydroperoxide compound with at least one hydroperoxide groups attached to a tertiary carbon; (D) 5% (w / w) to 95% (w / w) of at least one finely divided filler; (E) 0% (w / w) to 25% (w / w) of one or more components selected from a solvent, a photoinitiator, a stabilizer, and / or an antimicrobial agent. One embodiment of the invention is a dental cementation or core buildup kit that has enhanced compatibility between the acidic primer / adhesive and the cement or core buildup material, and therefore enhanced adhesion to a tooth. The kit includes (I) an acidic primer / adhesive composition having a pH less than 4.5; and (II) a cement or core buildup material having the above described composition comprising components (A)-(E). Another embodiment of the invention is a method for adhering a prosthetic device to a tooth to restore the function and / or appearance of a diseased tooth. Another embodiment of the invention is a method of providing a temporary cement, crown and / or bridge, inlay, onlay, endodontic sealer, and / or an endodontic filling material, using the inventive composition.

A root canal is filled and / or sealed using a hydrophilic sealing composition, optionally in combination with other filling materials. The sealing composition may include one or more resins that promote adhesion to hydrophilic dental tissues: The composition may be introduced into the root canal using a narrow cannula coupled to a high pressure hydraulic delivery device. In the case where a chemical cure composition is used to seal the root canal, a chemical initiator can be used to cause the mixed composition to harden over time. Hardening of at least a portion of the composition can be accelerated by including a photoinitiator and irratiating the mixed composition with radiant energy (e.g., from a dental curing lamp).

A dental composition compatible with acidic dental primers / adhesives, the dental composition comprising (A) 5% (w / w) to 90% (w / w) of at least one polymerizable monomer having at least one ethylenically unsaturated vinyl, acrylate, or methacrylate group; (B) 0.01% (w / w) to 10% (w / w) of at least one compound of structure where each of R1, R2, and R3 may be the same or different and is independently selected from H, alkyl, alkoxyl, aryl, acyl, allyl, pyridyl, —OH, alkenyl, aralkyl, cycloalkyl, and heterocyclic structure containing O, S, or N; (C) 0.01% (w / w) to 10% (w / w) of at least one hydroperoxide compound with at least one hydroperoxide groups attached to a tertiary carbon; (D) 5% (w / w) to 95% (w / w) of at least one finely divided filler; (E) 0% (w / w) to 25% (w / w) of one or more components selected from a solvent, a photoinitiator, a stabilizer, and / or an antimicrobial agent. One embodiment of the invention is a dental cementation or core buildup kit that has enhanced compatibility between the acidic primer / adhesive and the cement or core buildup material, and therefore enhanced adhesion to a tooth. The kit includes (I) an acidic primer / adhesive composition having a pH less than 4.5; and (II) a cement or core buildup material having the above described composition comprising components (A)-(E). Another embodiment of the invention is a method for adhering a prosthetic device to a tooth to restore the function and / or appearance of a diseased tooth. Another embodiment of the invention is a method of providing a temporary cement, crown and / or bridge, inlay, onlay, endodontic sealer, and / or an endodontic filling material, using the inventive composition.

The object is to provide a novel use application of a dental pulp stem cell collected from a deciduous tooth or a permanent tooth. Disclosed is a composition for autotransplantation or allotransplantation, which is characterized by comprising a dental pulp stem cell collected from a deciduous tooth or a permanent tooth.

The present invention is directed to therapeutic multifunctional immature dental pulp stem cells (IDPSCs), and IDPSCs multi-lineage compositions. The invention is further directed to the use of IDPSCs and compositions to reduce the risk of and / or treat degenerative diseases or for other medicinal and aesthetic purposes.

The present invention provides a method and compositions for tooth regeneration. Both implants of adipose-derived stem cell and dental pulp stem cell are able to grow self-assembled new teeth in extraction sockets when adding BMP2. The regenerated tooth is not only structurally similar to a normal tooth, but also well-developed in vascular and nervous systems with functions of growth, communication, and sensation. They are natural living teeth derived from this invented implantation method without any engineering procedure. It is ready for clinical testing and may be applied to future dental clinics.

The invention discloses toothpaste for treating tooth shake and toothache, firming teeth and blacking hairs, comprising twelve Chinese herbal medicines and auxiliary materials such as cassia nomame, edible salt, rhizome of nutgrass flatsedge and the like. Effective components can be extracted by using a scientific method. The toothpaste disclosed by the invention can be used for effectively cleaning oral cavities and teeth, preventing and treating various tooth diseases and fully activating the regeneration function of dental pulp, thereby fundamentally solving the recurring illness of tooth shake, toothache and weak teeth; moreover, the toothpaste has the function of blacking hairs. Therefore, the toothpaste achieves the aims of re-stabilizing loosened teeth, re-solidifying weak teeth, preventing tooth falling, avoiding the tooth diseases forever, treating the teeth and protecting the teeth, has no toxic or side effects after being used for a long term and is convenient to carry and use.

The invention relates to the field of stem cells and discloses a culture method of deciduous tooth pulp stem cells. The culture method includes: taking pulp tissue, cutting the pulp tissue into pieces, and then adding I-type collagenase; digesting for 10-20min under conditions of 37 DEG C and 200rpm; stopping digestion of a serum-free culture medium; blowing and beating discrete cell mass to obtain single discrete cells; adding cell suspension to re-suspend the cells, and adjusting cell density; culturing the cells in a carbon dioxide incubator at temperature of 37 DEG C and with humidity of 95%; when the cells grow to be fused by 80-90%, using digestive liquid of trypsase to digest the cells before passage culture, wherein the cell suspension is composed of the serum-free culture medium, epidermal growth factor and basic fibroblast growth factor. The cells cultured by the method are good in shape, have tendency of fusiform cluster growing and are high in activity and quick in proliferation, good stem cell characteristics of the deciduous tooth pulp stem cells can be maintained, and the culture method is suitable for large-scale culture of the deciduous tooth pulp stem cells.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and / or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

The invention relates to an aquagel material which comprises 3-80 wt% of colloidal particle containing lactic acid polymer, 0.1-40 wt% of pharmaceutical active component, 0-80 wt% of assistant and the balance of water. The aquagel material is applicable to promoting bone healing and new bone formation in tooth cavities after implementation of tooth extraction. The bone healing is wound healing after implementation of tooth extraction; and the new bone formation is new bone formation in a tooth implantation process. The aquagel material can also be used for temporary sealing after cavity preparation and during dental pulp disease treatment in the tooth disease treatment process.

The invention features methods and compositions for promoting the growth and differentiation of dental pulp stem cells and the use of the differentiated cells for the treatment of orthopedic conditions.

The invention discloses an injectable tissue engineering building method for regenerating dental pulp dentin. The method comprises the following three steps: preparation of an injectable hyaluronic acid gel scaffold material, separation and cultivation of mesenchymal cells of tooth germs, building of cells and injectable scaffold material compounds in different microenvironments, material preparation of implants and histological detection. According to the method disclosed by the invention, the third generation of mesenchymal cells of tooth germs are seed cells for regenerating dentin pulp sample structures by using a tissue engineering method; TGF-beta1 plays a crucial role in building of the dentin pulp sample structures; meanwhile, the hyaluronic acid gel can be an injectable scaffold material and is suitable for injectable dental tissue engineering; the third generation of mesenchymal cells of tooth germs, the TGF-beta1 and the hyaluronic acid gel are combined and injected into an emedullated dental pulp cavity to build the injectable tissue engineering; thus, in situ regeneration of a dentin-pulp complex sample structure can be realized under the micro-trauma condition, and a new possibility is provided for in situ and physiological regeneration of the dentin-pulp complex in clinic.

The invention discloses a dopamine-hyaluronic acid coating material as well as a preparation method and application thereof in promoting specific adhesion of dental pulp stem cell to dentin, and belongs to the technical field of dental pulp regeneration. The preparation method comprises the following steps: sequentially adding raw materials including hyaluronic acid, 1-(3-dimethylamino propyl)-3-ethyl carbodiimide, N-hydroxyl succinimide and dopamine hydrochloride into deionized water according to a molar ratio of (1 to 10) to 2 to 2 to 1, and mixing uniformly; stirring at room temperature under nitrogen protection; adjusting the pH to 4-5 with hydrochloric acid or sodium hydroxide; reacting for 2-5 hours while keeping the pH value unchanged; reacting overnight at room temperature; finally, performing dialysis and purification on the obtained reaction solution for 3-5 days in an acid deionized water environment; collecting the product; performing vacuum freeze drying for 48-96h, wherein the obtained white flocculent grafted product is a dopamine-hyaluronic acid composite material. The coating thickness is controlled by changing the molar ratio of the raw materials as well as the product concentration, and the coating material can effectively promote specific adhesion of dental pulp stem cell to the root canal wall.

The invention discloses a manufacturing method of a biological tooth root bracket material. The method comprises the following steps of: flushing teeth with phosphate buffered saline (PBS) in a sterile state for three times; removing dental crown parts by grinding with a carborundum disc, and keeping tooth root parts; pulling pulp tissues out of the tooth roots and repeatedly flushing the inside of root canals with physiological saline; gradually expanding the inside of teeth with a dental expander and repeatedly flushing the inside of the root canals with 5 to 17 percent of ethylene diamine tetraacetic acid (EDTA) solution; grinding outer side tissues of the tooth roots by grinding with a dental turbine and constructing a biological tooth root profile as required so as to obtain a bracket with a specific shape; preserving the teeth in the PBS and treating in an ultrasonic oscillator for 3 to 8 times; performing stepped demineralization on a dentine with EDTA with different concentrations in a magnetic stirrer with the rotating speed of between 100 and 500 revolutions per minute so as to obtain a dentine substrate; and preserving the dentine substrate in double-antibody-containing PBS. The biological tooth root bracket material has a tooth root profile, the thickness of 1mm to 3mm, the length of 1cm to 2.5cm and certain tooth inducing capability, comprises a large number of proteins and factors related to the manufacturing of teeth, can release the proteins and factors continuously, can better solve the problem of induction micro-environment, and contributes to application to tooth socket intra-alveolar orthotopic transplantation.

The invention relates to a gel material which comprises 3-80 wt% of sucrose acetate isobutyrate colloidal particle, 0.1-30 wt% of pharmaceutical active component, 0-60 wt% of assistant and the balance of water. The gel material is applicable to promoting bone healing and new bone formation in tooth cavities after implementation of tooth extraction. The bone healing is wound healing after implementation of tooth extraction; and the new bone formation is new bone formation in a tooth implantation process. The gel material can also be used for temporary sealing after cavity preparation and during dental pulp disease treatment in the tooth disease treatment process.

The invention belongs to the field of modified materials, and particularly relates to a long-acting antibacterial material and a preparation method thereof, a long-acting antibacterial dental diaphragm and a long-acting antibacterial invisible appliance. The long-acting antibacterial material provided by the invention is prepared by carrying out tertiary amination and in-situ quaternization on a base material of which the surface is polyurethane. According to the invention, tertiary amination and in-situ quaternization treatment are carried out on polyurethane (TPU) on the surface of a base material, so that quaternary ammonium salt antibacterial groups are grafted into a molecular structure on the surface of the base material, and the material has a stable and lasting antibacterial effect. The dental diaphragm and the invisible appliance provided by the invention are both made of the long-acting antibacterial material, so that the dental diaphragm and the invisible appliance have goodbiological safety and stable and lasting antibacterial effect, and can effectively prevent and treat oral diseases such as dental caries, periodontal diseases and dental pulp diseases.

A pulp chamber locator assists a dentist in performing a root canal procedure. The device includes a generally circular plate and a plurality of struts extending from the plate. The plate has a hole in the vicinity of its center. The device is inserted over a tooth, such that the struts engage the cemento-enamel junction of the tooth. The hole then indicates the proper point of entry for a dental instrument. The device may also include a rotatable indicator for showing the proper angle of entry, to insure that the instrument reaches the pulp chamber of the tooth. The dentist adjusts the position of the indicator, based on information provided by an X-ray or equivalent, and positions the dental instrument so as to be generally parallel with the indicator.

The invention discloses a pulp capping agent with bioactivity and a preparation method thereof. The pulp capping agent with bioactivity comprises dentin extraction solution and dentin powder. The pulp capping agent preparation method comprises the following steps: (1) selecting health dental tissue, cleaning by PBS (Phosphate Buffer Solution) to remove periodontal ligament, cementum and dental pulp, and sterilizing; (2) freezing the dental tissue, and grinding the frozen dental tissue to particles; (3) culturing the dental tissue particles in a medium; and (4) filtering the supernatant from the step (3) to obtain the dentin extraction solution, centrifugally cleaning the cultured dentin particles by the PBS, oven-drying, and grinding to obtain the dentin powders. The pulp capping agent can effectively promote the repair of dentin only by blending the dentin extraction solution with the dentin powder, and applying the mixture at a position close to the dental pulp or at a position at which the dental pulp is exposed, thereby improving the success rate of live-marrow conservation, and providing a new treatment means for patients suffering from accident pulp perforation.

A supporting element (1) for dental prostheses (2) can be applied in the root canals (3) of teeth treated with endodontic techniques and comprises a post (4) with an inhomogeneous radial cross-section designed to form, along its longitudinal axis (X), a duct (5) which forms a guide for the passage of an instrument (6) for treating the root canal (3) or an instrument (7) for removing the post (4) from the root canal (3) for subsequent treatment.

The invention discloses a preparation method of a super-bionic soft and hard tissue composite scaffold. The preparation method comprises the following steps: designing a bionic bone tissue scaffold, preparing a printing raw material, preparing printing ink, performing DLP photocuring 3D printing, performing freeze drying, extracting dental pulp mesenchymal stem cells of a patient, performing directional differentiation, inoculating and culturing the cells, preparing a soft tissue scaffold and preparing a soft and hard tissue composite scaffold. Through 3D printing of the super-bionic biological material, simultaneous personalized repair of oral clinical soft and hard tissues is achieved, development of a second operation area is avoided, and the treatment time for stage repair of the soft and hard tissues at present is shortened. Meanwhile, the three-dimensional microstructure of the jawbone tissue and the Harvard tube and the Walkman tube simulating the jawbone are inspired to establish good blood supply in the early stage, conditions are provided for layered induction of the bionic jawbone tissue, and a new thought is provided for design, research and development of the bionic scaffold.

The invention relates to the field of biotechnology, and in particular to application of dental pulp mesenchymal stem cells in treatment of Alzheimer disease. According to the invention, through experimental research, it is found for the first time that transplantation of dental pulp mesenchymal stem cells can inhibit chronic inflammation of the brain, improve learning and memory capacity and movement capacity to a certain extent, reduce number of microglia in each region of the hippocampus, and effectively treat Alzheimer disease.

The invention provides application of cytokines in promotion of exosome secreted by dental pulp stem cells. According to the invention, cytokines are used as stimulants; five factor receptors selectedby the invention are expressed on the surfaces of the dental pulp stem cells and the five cell factors can stimulate proliferation, differentiation or migration of the dental pulp stem cells, whereinthe processes of cell proliferation, differentiation, migration and the like are all processes of cell activation and vigorous cell metabolism. The cells can secrete lots of exosomes in an activatedstate. Therefore, after the dental pulp stem cells are stimulated by the five cytokines to be in an activated state, so that the exosome secretion amount of the dental pulp stem cells is remarkably increased; and the obtained exosome can be used for anti-aging or other medical purposes and has great market value.

The invention discloses a method for performing interceptive treatment on osteoarthritis of knee joints through dental pulp stem cells (DPSCs). The method comprises the following steps: injecting CFA(Complete Freund's Adjuvant) and MIA (Sodium Iodoacetate) through an articular cavity and performing induction for one week to obtain a rat model of the osteoarthritis of the knee joints; performing intervention and treatment on different groups of rats after one week, wherein a treatment group adopts interceptive treatment in a way of DPSCs cell suspension liquid articular cavity orthotopic injection or intravenous injection; taking materials after treatment for 4 weeks and analyzing a treatment effect, and evaluating the treatment effect of DPSCs on the osteoarthritis of the knee joints. According to the method disclosed by the invention, an experimental result proves that the DPSCs has a treatment effect on bone osteoarthritis induced by combination of CFA and MIA; after treatment for 4weeks, through DPSCs systematic or local injection, a knee joint bone structure induced by the combination of CFA and MIA can be recovered, and exudates of the articular cavity are reduced. Through the DPSCs systematic or local injection, a transparent cartilage arrangement structure and cell forms can be recovered.

The invention relates to the application of a composition containing recombinant amelogenin and propylene glycol alginate (“PGA”) on to cut dentin tubules followed by installation of a definitive restorative in a single visit.

A root canal surgery robot comprises a movable control table, a root canal cleaner and a root canal filler, and automatic root canal surgical treatment for an affected tooth can be achieved under supervision of a doctor. The movable control table is used for receiving instructions of the doctor and controlling automatic operation of the root canal cleaner and the root canal filler. The multifunctional composite root canal cleaner adopts good wave conduction of liquid incompressibility, so that liquid medicines in a pulp chamber and root canal system generates adaptable multi-harmonic horizontal vibration suitable for the shape of a root canal, the pulp chamber of the affected tooth is thoroughly cleaned and disinfected, and root canal filling can be directly performed on the basis. The root canal has a small diameter, a long length and common irregular shapes, the multifunctional composite root canal filler can generate suitable negative pressure in a dental pulp and root canal system,hot gutta-percha is sucked into the root canal by the negative pressure, so that preparation operation of the root canal is omitted, root canal filling quality is improved, and healthy dental tissuescan be furthest protected.