178 results about "Pulpa dentis" patented technology

The invention belongs to the field of hydrogel preparation, and relates to a preparation method of a polypeptide photo-crosslinking hydrogel material loaded with human dental pulp stem cells, and the method comprises the following steps: uniformly mixing methacrylic acid modified BFP1 polypeptide with a GelMA hydrogel solution and human dental pulp stem cells, and then carrying out photo-crosslinking under the action of a photoinitiator; finally, the polypeptide photo-crosslinking hydrogel material loaded with the human dental pulp stem cells is formed. According to the invention, methacrylic acid modified BFP1 polypeptide and a GelMA hydrogel material are photo-crosslinked for the first time and are combined with human dental pulp stem cells for bone repair, so that the bone repair effect is improved; the obtained polypeptide hydrogel material has excellent stem cell carrying capacity.

Apparatus and methods for endodontic treatment of teeth provide effective cleaning of organic material (such as pulp and diseased tissue) from the root canal system. In an embodiment, a compressor system generates high pressure liquid (e.g., water) that flows through an orifice to produce a high-velocity collimated jet of liquid. The high-velocity jet is directed toward a surface of a tooth, for example, an exposed dentinal surface, and impingement of the jet onto the surface generates an acoustic wave that propagates throughout the tooth. The acoustic wave effectively detaches organic material from dentinal surfaces and tubules. The detached organic material is flushed from the root canal system by the liquid jet and / or by additional irrigation.

The invention is applicable to the technical field of cell engineering, and provides a mitochondria-enhanced dental pulp mesenchymal stem cell and a preparation method and application thereof, and the preparation method comprises the following steps: co-culturing platelet-rich fibrin and dental pulp mesenchymal stem cells by using a Transwell chamber to obtain the mitochondria-enhanced dental pulp mesenchymal stem cell. The platelet-rich fibrin transmits mitochondria to the dental pulp mesenchymal stem cells to improve the regeneration capacity of the dental pulp mesenchymal stem cells. According to the invention, an autologous platelet enrichment, namely platelet-rich fibrin, is used as a mitochondria donor with a respiratory function, mitochondria is continuously transferred to the dental pulp-derived mesenchymal stem cells through a cell bioengineering technology, and the mitochondria-enhanced dental pulp mesenchymal stem cells are constructed and applied to tissue damage repair; the vascular regeneration regulation and control capability of the dental pulp mesenchymal stem cells is obviously improved, and the existing mesenchymal stem cell treatment technology is obviously improved.

The invention relates to the technical field of dental oral treatment, and discloses a repair method for treating periodontitis by using dental pulp stem cells, which comprises the following steps: S1, extracting dental pulp tissues from the oral cavity of a patient; s2, obtaining dental pulp stem cells; s3, culturing the dental pulp stem cells under specific conditions; s4, inducing the dental pulp stem cells to differentiate in the direction of osteogenesis, chondrogenesis or periodontal ligament cell formation; s5, transplanting the differentiated dental pulp stem cells to a periodontitis injury area of the patient, and using a biocompatible material; s6, applying a proper local stimulation means around the cell transplantation area; s7, by applying anti-inflammatory drugs locally or systemically, local immune response and inflammatory response are retarded, and repair and regeneration of periodontium are promoted. The problems that an existing treatment method is not lasting in effect, long in recovery period and the like are solved, the damaged periodontium is repaired through the dental pulp stem cells, the treatment effect can be remarkably improved, and the dental pulp stem cells have high clinical application value.

The invention discloses application of Axin1 in regulation and control of osteogenic differentiation of dental pulp stem cells of deciduous teeth. The research shows that the overexpression and knock-down of Axin1 do not influence the proliferation of SHED and the characteristics of MSC. However, overexpression of Axin1 promotes migration of SHED, and this effect can be weakened by Axin1 silencing. Overexpression of Axin1 promotes osteogenic differentiation of SHED, and knock-down of Axin1 shows a reverse effect. In mechanism, overexpression of Axin1 obviously improves the expression level of phosphorylated ERK1 / 2, so that osteogenic differentiation of SHED is promoted. The promotion effect of overexpression of Axin1 on osteogenic differentiation of SHED can be eliminated by using an ERK1 / 2 chemical inhibitor for treatment, which indicates that Axin1 plays a role as a key regulatory factor for osteogenic differentiation in SHED through an ERK1 / 2 pathway. A research result shows that Axin1 can be used as a potential new target and is used for treating diseases related to bone regeneration by applying SHED.

This cell activator comprises a fraction isolated from a culture supernatant of dental pulp stem cells of milk teeth, wherein the fraction does not contain a component of less than 3.5 kDa.

The invention belongs to the technical field of dental pulp blood supply reconstruction, and relates to a multi-omics data fusion analysis method for EDTA (Ethylene Diamine Tetraacetic Acid) induced cell differentiation in dental pulp blood supply reconstruction. A space-time matched multi-level data basis is provided for analyzing a dynamic rule; then through standardization processing and difference analysis, screening out differential molecules which realize corresponding modes and are strongly related to phenotypes, focusing on core regulation and control candidate objects, and avoiding limitation of single omics; the method comprises the following steps: constructing a dynamic multi-layer regulation and control network comprising a transcription layer, a protein layer, a metabolism layer and interlayer connections thereof, finally identifying key nodes and modules through network topology analysis, tracking a time evolution rule in combination with dynamic trajectory analysis, and finally screening a core regulation and control module and predicting a cross-level key path. System analysis from multi-omics data to a hierarchical regulation and control mechanism and a dynamic rule is realized, and the limitation of single-dimension research is effectively broken through.

A method and apparatus for detecting a distance of a probe contacting a tooth surface to a pulp of a tooth by determining an indication of a thickness of a dentin of the tooth is provided. The method includes illuminating tooth tissue of the tooth with the probe and then receiving data indicative of scattered light from the tooth tissue. A spectrum of the scattered light is then acquired and analyzed to further acquire a spectral analysis result. An indication of the thickness of the dentin of the tooth is then determined based on the spectral analysis result. The method can help prevent accidental exposure of the pulp during dental treatments such as drilling.

The invention discloses a preparation method of an SHED-HUVEC organoid, an injection type compound and application of the injection type compound. The preparation method comprises the following steps: a, extracting and culturing SHED; b, preparing a spheroid culture model micropore disc; c, constructing an SHED spheroid; d, activity detection; e, an SHED-HUVEC type organ is constructed; and f, dynamically monitoring the cells. According to the preparation method, the SHED-HUVEC organoid is constructed under the condition of not additionally adding growth factors, that is, the defects caused by using the growth factors can be reduced, the SHED-HUVEC organoid can remarkably promote the regeneration of vascularized dental pulp tissues, so that the regenerated dental pulp tissues form more mature blood vessels, and the regeneration rate of the dental pulp tissues is increased. The vascularization level and functional recovery of regenerated dental pulp tissues are improved. The injection type compound is prepared by mixing an SHED-HUVEC organ and a biocompatible hydrogel, and the injection type compound is applied to dental pulp regeneration treatment.

Embodiments of the present application disclose a method and device for extracting root canal feature morphology based on CBCT data, equipment and medium, belong to the field of oral medicine and medical image technology, wherein the method comprises: filtering and denoising CBCT images and performing pulp segmentation processing, and performing surface rendering three-dimensional reconstruction on tooth root canals to generate a three-dimensional model; based on the regular point cloud data in the three-dimensional model, a point-surface method is used to realize surface reconstruction to generate a root canal entity model; the root canal entity model is subjected to coordinate alignment processing in a three-dimensional space, and the approximate center line of the fitted root canal is used to measure the root canal length, root canal opening direction, root canal curvature and pulp depth. The present application quickly reconstructs the corresponding three-dimensional model based on the CBCT tomographic images obtained by scanning the teeth of a patient, and obtains relevant data such as pulp depth and root canal opening size from the three-dimensional model of the teeth to assist the performance of oral surgery.

The utility model discloses an emulational resin tooth matched with pulp opening treatment in oral skill examination, which comprises an emulational resin tooth body, the emulational resin tooth body is provided with a plurality of dental pulp walls and a root canal, a dental pulp chamber is enclosed by the plurality of dental pulp walls, the surface of each inner wall of the dental pulp walls is provided with a contour coating, and the root canal is arranged on the outer wall of the dental pulp chamber. The root canal is filled with fluid, and the mouth of the root canal is closed. When the simulated resin tooth is used for an examination, a review teacher can quickly and accurately judge whether an examinee uncovers and uncovers the top wall of the dental pulp or not, check the abrasion conditions of other walls of the dental pulp and determine whether the bottom wall of the dental pulp is perforated or not by directly observing the outline coating; in addition, whether the examinee finds the position of each root canal opening or not can be determined by checking whether fluid overflows from each root canal opening or not, the simulation resin tooth can assist a review teacher to objectively and accurately evaluate the practical operation ability of the examinee, the review difficulty and blindness of the review teacher are reduced, and the review accuracy and efficiency are effectively improved.

The invention relates to the technical field of dental pulp observation, in particular to a dental pulp observation device and a lifting assembly, the dental pulp observation device comprises a support, a sliding rod in sliding connection with the support and a containing cabin arranged at the end, away from the support, of the sliding rod, and the containing cabin comprises an operation channel; the first cavity and the second cavity are arranged in the support, the first cavity is communicated with the second cavity, the first piston rod is arranged in the first cavity in a sliding mode, the second piston rod is arranged in the second cavity in a sliding mode, and the second piston rod is connected with the sliding rod. The first elastic piece is arranged in the second cavity; the rotating piece is arranged on the outer wall of the support and is in meshed connection with the first piston rod; by controlling the flowing of liquid oil between the two chambers, the equipment can automatically move and also can be fixedly used; the use space of the equipment can be saved, and proper and comfortable positions can be found according to different patients.

The invention discloses a female vaginal mucosa cell maintenance preparation and a preparation method and application thereof, and belongs to the technical field of biological agents, the female vaginal mucosa cell maintenance preparation is prepared from dental pulp mesenchymal stem cells, portulaca oleracea polysaccharide, lactic acid, quercitrin, glycerin and stem cell carriers, and vaginal injection paste is obtained by evenly mixing the dental pulp mesenchymal stem cells, the portulaca oleracea polysaccharide, the lactic acid, the quercitrin, the glycerin and the stem cell carriers through a proper amount of deionized water. The purslane polysaccharide and the dental pulp mesenchymal stem cells form a unique synergistic effect, through physical wrapping, the survival rate and delivery efficiency of the stem cells in a vaginal cavity are improved, local inflammatory response is inhibited, a microenvironment is optimized to support directional differentiation of the stem cells into epithelial cells, paracrine activity of the stem cells is enhanced, and repairable factors are released to promote cell-level regeneration. The problem of hormone dependence is solved, deep maintenance of targeted mucous membrane cells is achieved, limitation of an existing therapy is broken through by directly nourishing cells, activating and regenerating, enhancing barrier integrity and maintaining an acidic microenvironment, and an ideal treatment effect is achieved.

The invention belongs to the field of tooth and dental pulp, and particularly relates to a tooth and dental pulp treatment whole-process simulation tooth and jaw model which comprises an upper jaw model, an upper jaw base, an upper jaw joint, a lower jaw model, a lower jaw base, a lower jaw joint, a connecting device, a neck joint and a tail fixing device. The lower jaw model comprises a lower jaw shell, a movable fine adjustment module and extracted teeth; the lower jaw shell is filled with a gel material capable of absorbing water; the movable fine adjustment module is a detachable fine adjustment module. The device can quickly fix and replace human isolated teeth, also can restore the adjacency relation of the isolated teeth, the periodontal microenvironment and the moist conductive environment around the tooth root, can highly simulate the physiological conditions of the isolated teeth in dentition and jaw bone, is suitable for various teaching trainings such as tooth pulp disease microscopic root canal treatment, tooth restoration, microscopic root apex surgery and the like, and has a wide application prospect. And the application scene is greatly widened.

The invention relates to the technical field of biological medicine, and provides application of a dental pulp stem cell exosome in preparation of a medicine for treating and preventing osteoarthritis, a preparation method of the dental pulp stem cell exosome comprises the following steps: S1, obtaining dental pulp tissue from a dental pulp cavity of an in-vitro tooth; s2, pretreating dental pulp tissues, adding digestive juice, digesting, centrifuging, discarding supernatant, adding a complete culture medium containing antibiotics into precipitates, mixing, filtering and centrifuging to obtain dental pulp stem cells; and S3, culturing the dental pulp stem cells by using a complete culture medium, when the confluence degree of the dental pulp stem cells reaches 60-80%, replacing the serum-free culture medium for continuous culture, collecting the dental pulp stem cell conditioned culture medium, and performing differential centrifugation to obtain the dental pulp stem cell exosome. According to the technical scheme, the regulating effect of the dental pulp stem cell exosome on endoplasmic reticulum stress and the mechanism of the dental pulp stem cell exosome for improving cartilage cell degeneration are explored.

Apparatuses and methods are provided for displaying the position of a dental file during endodontic treatment. Data showing the morphology of a tooth is obtained. In one embodiment, the data is X-ray image data. A three-dimensional representation of the dental file positioned in the tooth is generated, and a length that the dental file extends from an upper surface of the tooth is determined from the three-dimensional representation. A representation of the dental file is provided in the image of the tooth obtained from the data showing the tooth morphology, with the representation of the dental file being depicted such that a part of the representation of the dental file extends from the upper surface of the tooth in proportion to the length that the dental file extends from the upper surface of the tooth as determined from the three-dimensional representation and such that a remaining part of the representation of the dental file extends into the tooth, with a part of the remaining part of the representation of the dental file being positioned in the root of the tooth in the image. The image of tooth and representation of the dental file may be displayed.

The utility model discloses a special intramedullary file of minimally invasive, relates to the technical field of intramedullary file, including file main part, the one end fixed connection of file main part has micro motor, the outer wall fixed connection of micro motor has handle, the outer wall movable joint of handle has heat dissipation mechanism, and heat dissipation mechanism includes ring, water pipe and transmission strip, and the both sides fixed mounting of ring have protruding block, and the one side of protruding block is fixedly connected with the one end of transmission strip, the utility model discloses the special structure design of heat dissipation mechanism, can utilize water pipe to radiate heat when intramedullary file works, reduces the heat damage risk of heat of the generation of filing to dental pulp tissue, guarantees postoperative recovery, and simultaneously, the connection and sliding relation among each part make heat dissipation mechanism can move flexibly, and the different filing operation demand of adaptation.

Provided are a dental pulp mesenchymal stem cell-microcarrier scaffold complex, and a preparation method therefor and a use thereof. The dental pulp mesenchymal stem cells and a microcarrier are subjected to 3D culture and amplification under low oxygen and high osmotic pressure conditions, and then sampling and counting are carried out, and a dental pulp mesenchymal stem cell-microcarrier scaffold complex is directly subjected to stem cell liquid nitrogen cryopreservation. The dental pulp mesenchymal stem cell-microcarrier scaffold complex can secrete higher cytokines and exert better anti-inflammatory and immunomodulatory effects. The complex can be directly cryopreserved without degradation of a scaffold and preparation of single cells, so that the operation is simple, and the activity and the survival of the cells which are resuscitated from cryopreservation are higher. Before cell treatment, the dental pulp mesenchymal stem cell-microcarrier scaffold complex is directly thawed, has auxiliary materials added, and is placed into a biological artificial liver reactor for treating acute liver failure.

The utility model belongs to the technical field, and provides a cleaning drill for cleaning tooth pulp, which comprises a grip, a drill bit, a box body, a suction head, a connecting pipe and a collecting box, one end of the grip is provided with a shell, the suction head is connected with the shell, the connecting pipe is connected with the suction head and extends into the shell, a connecting seat is arranged in the shell, and the collecting box is connected with the connecting seat. A cylinder is arranged on the shell, the collecting box is arranged in the box body, the interior of the box body is of a hollow structure, a cleaning mechanism is arranged in the box body, and a driving mechanism is arranged in the shell. The problems that sundries and water mist cannot be collected, automatic cleaning cannot be carried out, and pollution is large when waste is discharged are solved.

The invention discloses an oral cavity teaching training model for dental pulp blood supply reconstruction, and the model comprises a model body which is provided with a puncture channel for simulating a root canal; the puncture seat is arranged at the lower end of the puncture channel, and a bleeding hole communicated with the puncture channel is formed in the puncture seat; the simulation film is arranged between the puncture channel and the bleeding hole; the driving mechanism is connected with the simulation thin film, and the driving mechanism is used for driving the simulation thin film to move so as to adjust the puncture area, corresponding to the bleeding hole, of the simulation thin film; the liquid supply device is connected with the bleeding hole, the liquid supply device is used for conveying simulated plasma to the bleeding hole, or the liquid supply device is used for conveying simulated plasma in the bleeding hole outwards, the simulated film is matched with the bleeding hole to achieve high simulation of bleeding after puncture, and meanwhile dynamic simulation of different bleeding amounts is achieved in cooperation with the liquid supply device; through cooperation of the driving mechanism and the simulation thin film, rapid switching of the puncture areas corresponding to the simulation thin film is achieved, and repeated training is facilitated.

The invention relates to the technical field of oral medicine, in particular to a DNMTi and ZIF-8 nano-complex and application thereof.The DNMTi and ZIF-8 nano-complex is technically characterized in that in-situ synthesized zeolite imidaze-8 (ZIF-8) is adopted for delivering a DNA methyltransferase inhibitor (DNMTi), dental pulp stem cell (DPSC) cell spheres are combined, the DNMTi and ZIF-8 nano-complex can be used for bionic therapy, the activity of medicine is prolonged, and the effect of treating dental pulp stem cells is achieved. And zinc ions are continuously released to activate a PI3K-AKT signal channel, so that the dentin differentiation of stem cells and the generation of blood vessels are promoted. Through accurate simulation of a tooth development microenvironment, the biomimetic therapy can realize regeneration of a dental pulp-dentin complex. The therapy for simulating the tooth development process not only solves the key problems in the dental tissue engineering, but also opens up a new direction for treating the dental pulp and periapical diseases, and highlights the wide application prospect in regenerative medicine.

This invention provides a treatment system for promoting dental pulp repair using oral stem cells, belonging to the field of oral treatment technology. The system utilizes a dynamic gene regulation module, an adaptive intelligent scaffold, and a bio-digital fusion interface in synergy. Through the dynamic gene regulation module and the adaptive intelligent scaffold, the efficiency and therapeutic effect of dental pulp regeneration are significantly improved. The dynamic gene regulation module employs a light-controlled CRISPR system to perform real-time gene editing on dental pulp stem cells, dynamically adjusting according to the needs of the regeneration stage. The adaptive intelligent scaffold, based on 3D printing technology and manufactured using a digital model of the patient's teeth, can dynamically release growth factors and optimize the microenvironment. This combination of personalized design and dynamic regulation allows for adaptive adjustments to meet the specific needs of the patient, not only improving the repair efficiency of dental pulp tissue but also enhancing the success rate of functional reconstruction. This provides patients with dental pulp injury with a more efficient and precise treatment option, promoting the personalized development of oral regenerative medicine.

The utility model relates to the technical field of medical apparatus and instruments, and discloses a tooth and pulp anesthesia apparatus which comprises a main body mechanism, a safety mechanism and a lighting mechanism, a pushing part is arranged at the rear end of a connecting rod, a second non-slip mat is arranged at the rear end of the pushing part, a push rod is arranged in a needle cylinder, and a second non-slip mat is arranged at the rear end of the pushing part. The main body mechanism comprises a plurality of key components, the needle cylinder is used for containing and conveying injection liquid, the power-assisted plate provides additional holding force for a user and facilitates pushing of the needle cylinder, the first non-slip mat and the second non-slip mat are located at the rear end of the power-assisted plate and the rear end of the pushing part and increase the stability during holding, and the connecting rod is connected with the pushing part and the needle cylinder. And the push rod is used for transmitting pressure of a user to the push part to the inside of the needle cylinder. According to the tooth and dental pulp anesthesia apparatus, through the built-in safety mechanism, the apparatus is automatically locked after being used for the first time, the needle cylinder is effectively prevented from being repeatedly used, the risk of cross infection is greatly reduced through the design, and the safety of patients and medical staff is ensured.

The utility model provides a dental pulp root canal auxiliary positioning device which comprises a handle, a mounting box, a triangular block, a supporting plate and a butt joint shaft, the lower end of the handle is sleeved with a roller bearing, the outer ring of the roller bearing is fixedly connected with an anti-skid sleeve, guide grooves are formed in the front side and the rear side of the right end of the mounting box respectively, and a rotating shaft is mounted at the lower end in the mounting box; the annular side face of the rotating shaft is fixedly sleeved with a plurality of second rolling wheels, a notch column is movably connected to the upper portion of the installation box in an inserted mode, a first rolling wheel is rotatably installed at the lower end of the notch column, a circular ring is fixedly connected to the side face of the notch column in a sleeved mode, and a spring is installed on the side of the upper portion of the circular ring and connected with the notch column in a sleeved mode. The handle placing device is reasonable in structure, good in practicability, convenient to use and suitable for popularization and application, the handle is limited by utilizing the telescopic characteristic of the spring, and the stability of the handle during placing is greatly improved.

The invention discloses a preparation method and application of a graded delivery system which takes a pH response type metal organic framework material NH2-MIL-101 (Fe) as a carrier and takes antibacterial peptide AMP and irisin as active ingredients. The graded delivery system comprises a metal organic framework material NH2-MIL-101 (Fe), AMP (adenosine monophosphate) and Irisin, wherein the AMP and the Irisin are loaded on the pH (potential of hydrogen) response type metal organic framework material NH2-MIL-101 (Fe). According to the system, aiming at periodontal and dental pulp infectious diseases, early-stage AMP rapid release for precise bacteriostasis and middle-stage Irisin synergistic release are realized through time sequence regulation and control to achieve anti-inflammatory and anti-oxidation effects, so that alveolar bone tissue / dentin regeneration is further promoted, and an excellent'antibacterial-anti-inflammatory-hard tissue regeneration 'cascade treatment effect is shown. The invention provides a new strategy and theoretical basis for precise treatment of periodontitis and pulpitis.

The invention provides an auxiliary positioning device for an oral dental pulp root canal, and relates to the technical field of root canal treatment equipment. Comprising a positioning platform and a positioning frame, and the positioning platform is arranged on the positioning frame; a positioning clamp is arranged on the positioning platform, a positioning sleeve is connected to the positioning clamp through an induction line, and an inductance device is arranged on the positioning sleeve; displacement assemblies are arranged at the tail ends of the positioning clamps; the displacement assembly comprises an outer pipe and an inner pipe, and one end of the outer pipe is sleeved with one end of the inner pipe. A first motor is arranged in the outer pipe, a driving screw rod is arranged on an output shaft of the first motor, and threads are arranged on the inner side of the inner pipe and are in threaded connection with the driving screw rod; a limiting groove is formed in the inner side of the outer pipe, and a limiting protrusion matched with the limiting groove is arranged on the outer side of the inner pipe. Compared with the prior art, the device is convenient to use and adjust, accurate in positioning and capable of effectively avoiding blocking the surgical field.

The invention relates to a digital design method and device for a dental restoration and a storage medium. The method comprises the following steps: S1, respectively obtaining point clouds of enamel, dentin and dental pulp of a tooth to be restored and a reference tooth thereof by using a CBCT (Cone Beam Computed Tomography) image; s2, determining a tooth body center point of the reference tooth based on tooth enamel, dentin and tooth tip positions of dental pulp of the reference tooth, and determining a tooth body center point of the to-be-repaired tooth in combination with a first volume ratio of the reference tooth to the to-be-repaired tooth; s3, respectively making infinitely long cones by taking the central point of the tooth body as the vertex of the to-be-repaired tooth and the reference tooth to obtain volume coefficients of dentin and enamel of the reference tooth and the to-be-repaired tooth; and S4, gradually enlarging the vertex angle of the cone, and reconstructing enamel and dentin of the to-be-repaired tooth layer by layer. Compared with the prior art, the method has the advantages that the complex geometrical morphology and biomechanical characteristics of enamel and dentin are accurately simulated based on CBCT images, the weights of all levels are set, and bionic repair better conforming to natural teeth is achieved.

The invention relates to a three-platinum-root-rehmannia root gargle and a preparation method and application thereof, and belongs to the technical field of oral medicine and traditional Chinese medicine application. The invention provides a three-platinum radix rehmanniae gargle. The three-platinum radix rehmanniae gargle comprises the following components in parts by mass: 1-10 parts of rhizoma bletillae, 1-10 parts of radix angelicae, 1-10 parts of rhizoma corydalis, 1-10 parts of cortex dictamni, 1-10 parts of fructus kochiae, 1-10 parts of honeysuckle, 1-5 parts of borneol and 1-5 parts of pseudo-ginseng. The three-platinum-radix rehmanniae gargle provided by the invention locally acts on sensitive areas of diseased teeth and is gargled after treatment, so that the three-platinum-radix rehmanniae gargle has the effects of diminishing inflammation, inhibiting bacteria, resisting tooth sensitivity, dispersing blood stasis, stopping bleeding, promoting tissue regeneration, relieving pain, clearing away heat and toxic materials and the like, effectively relieves tooth pain or sensitivity and protects dental pulp. When being used for preparing the deep caries pulp-protecting treatment medicine, the compound can permeate into dentin or dental pulp in the deep caries pulp-protecting treatment process and after operation, and plays the roles of relieving pain, desensitizing, clearing away heat and toxic materials and the like, so that the dental pulp is protected, and toothache and tooth sensitivity are avoided.

This invention proposes a method for preparing dental pulp stem cell exosomes and their applications, belonging to the field of exosome technology. Dental pulp stem cells from exfoliated deciduous teeth are seeded into serum-free α-MEM medium containing microparticles, insulin-like growth factor, transforming growth factor-β1, penicillin, and streptomycin for culture. Exosomes are isolated, dispersed in PBS solution, and incubated with microparticles, propylene glycol, and rhodioloside to obtain highly active dental pulp stem cell exosomes. The surface of these exosomes is then modified with shikonin and interferon to obtain dental pulp stem cell exosomes. The dental pulp stem cell exosomes prepared by this invention exhibit good affinity for titanium implant-mediated osteointegration repair materials, demonstrating good anti-inflammatory, immunomodulatory, osteogenic, and damage-reducing effects. Furthermore, the preparation method is simple, yields high output, and operates under mild conditions, showing broad application prospects.

The invention discloses an in-vitro extraction and amplification method of a vascular development related dental pulp stem cell subpopulation, which comprises the following steps: extracting primary DPSCs from a dental pulp tissue through trypsin and collagenase digestion, and preparing a single-cell suspension; sorting the cells using flow cytometry; and the PDGFR beta + DPSCs are sorted out through a flow cytometer. And finally, inoculating the separated PDGFR beta + DPSCs into a DMEM culture medium containing 10% of FBS and 1% of penicillin-streptomycin, and culturing under the conditions of 37 DEG C and 5% of CO2. The PDGFR beta + DPSCs purified and cultured through the method have high proliferation, migration and angiogenesis promoting performance. According to the method, autologous dental pulp stem cells which are from donors of different ages and under different conditions and have high angiogenesis promoting characteristics can be stably obtained in a clinical environment to a certain extent. The method provides a necessary research basis for exploring and maintaining a regulation mechanism of DPSCs self-renewal, stable amplification and promotion of vascular performance, further widening the clinical application prospect of DPSCs and promoting dental pulp regeneration treatment.