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Application of cytokines in promotion of exosome secreted by dental pulp stem cells

A technology of dental pulp stem cells and cytokines, which is applied to medical preparations containing active ingredients, animal cells, vertebrate cells, etc., can solve the problems of research, no promoting factors, and no stem cell culture, etc., and achieves broad market value, The effect of increasing secretion

Active Publication Date: 2020-03-17
中科细胞科技(广州)有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Chinese patent application publication number CN 106492194A discloses a stem cell exosome preparation and its preparation method and application. The cytokine used in it is only basic fibroblast growth factor, and the prepared preparation is used as exosome Supplement is an additional addition to exosomes. The patent uses dental pulp stem cells + exosomes + basic fibroblast growth factor (10ng / ml) as a mixed preparation to treat gastritis, and there is no secretion of stem cell exosomes The promotion factors of the study have not been specifically studied, nor have they involved the cultivation of stem cells that can be used in the skin

Method used

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  • Application of cytokines in promotion of exosome secreted by dental pulp stem cells
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  • Application of cytokines in promotion of exosome secreted by dental pulp stem cells

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Embodiment 1

[0048] 1. Main equipment

[0049] Table 1

[0050]

[0051]

[0052] 2. Main reagents

[0053] Table 2

[0054] Reagent name Reagent manufacturers MSC Medium and Supplements Beijing Youkang Technology Co., Ltd. ɑ-MEM Beijing Youkang Technology Co., Ltd. FBS (Fetal Bovine Serum) Gibco Exosome CD63&TSG101 Protein Detection Kit Shanghai Yumeibo Biotechnology Co., Ltd. MSC Stem Cell Flow Cytometry Reagents BD Epidermal Growth Factor (EGF) Beijing Tongli Haiyuan Biotechnology Co., Ltd. Insulin-like Growth Factor (IGF-1) Beijing Tongli Haiyuan Biotechnology Co., Ltd. Platelet-derived growth factor (PDGF-BB) Beijing Tongli Haiyuan Biotechnology Co., Ltd. Stem Cell Factor (SCF) Beijing Tongli Haiyuan Biotechnology Co., Ltd. Recombinant Human Interferon-γ (IFN-γ) Beijing Tongli Haiyuan Biotechnology Co., Ltd. PBS Gibco dispase Gibco Collagenase Gibco Elisa kit for VEGF, TGF...

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Abstract

The invention provides application of cytokines in promotion of exosome secreted by dental pulp stem cells. According to the invention, cytokines are used as stimulants; five factor receptors selectedby the invention are expressed on the surfaces of the dental pulp stem cells and the five cell factors can stimulate proliferation, differentiation or migration of the dental pulp stem cells, whereinthe processes of cell proliferation, differentiation, migration and the like are all processes of cell activation and vigorous cell metabolism. The cells can secrete lots of exosomes in an activatedstate. Therefore, after the dental pulp stem cells are stimulated by the five cytokines to be in an activated state, so that the exosome secretion amount of the dental pulp stem cells is remarkably increased; and the obtained exosome can be used for anti-aging or other medical purposes and has great market value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a cytokine in promoting the secretion of exosomes from dental pulp stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of adult stem cells with osteogenic, chondrogenic and adipogenic functions. MSC can regulate immunity, promote angiogenesis and support hematopoiesis through paracrine. MSCs are rich in sources and exist in various tissues such as placenta, umbilical cord, bone marrow, dental pulp, and fat. Therefore, MSC as a drug has been approved by many countries for the treatment of diseases such as graft-versus-host disease, ulcerative colitis, and myocardial infarction recovery period. However, regardless of intravenous injection, intramuscular injection or intramyocardial injection, exogenously transplanted MSCs mostly died within 72 hours. Then, regarding how dead MSCs play the above-mentioned role, previous studies have ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/28A61P17/18
CPCC12N5/0664A61K35/28A61P17/18C12N2501/11C12N2501/105C12N2501/135C12N2501/125C12N2501/24Y02A50/30
Inventor 唐忆琳陈巧林
Owner 中科细胞科技(广州)有限公司
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