Method for performing interceptive treatment on osteoarthritis of knee joints through dental pulp stem cells

A technology of osteoarthritis and stem cells, applied in anti-inflammatory agents, bone diseases, pharmaceutical formulations, etc., can solve the problems of inability to apply in large quantities, low awareness, high cost, etc., and achieve the effect of reducing joint cavity exudate

Inactive Publication Date: 2018-05-18
上海卡智生物技术有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For severe OA that is ineffective in conservative treatment, joint replacement surgery can be considered,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for performing interceptive treatment on osteoarthritis of knee joints through dental pulp stem cells
  • Method for performing interceptive treatment on osteoarthritis of knee joints through dental pulp stem cells
  • Method for performing interceptive treatment on osteoarthritis of knee joints through dental pulp stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0025] like Figure 1 to Figure 3 Shown, a method for the blockade of dental pulp mesenchymal stem cells for the treatment of knee osteoarthritis, the method uses complete Freund's adjuvant (CFA) and sodium iodoacetate (MIA) through the joint cavity injection, after a period of 1 week The rat model of knee osteoarthritis was obtained by the induction of knee osteoarthritis; after 1 week, the rats in different groups were treated with intervention, and the treatment group was treated with DPSCs cell suspension in situ injection or intravenous injection to block the Treatment: After 4 weeks of treatment, samples were collected to analyze the treatment effect to evaluate the treatment effect of DPSCs on knee osteoarthritis.

[0026] 1. Experimental materials:

[0027] 1) Experimental animals: SD rats, female, 16, 8 weeks old, SPF grade.

[0028] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for performing interceptive treatment on osteoarthritis of knee joints through dental pulp stem cells (DPSCs). The method comprises the following steps: injecting CFA(Complete Freund's Adjuvant) and MIA (Sodium Iodoacetate) through an articular cavity and performing induction for one week to obtain a rat model of the osteoarthritis of the knee joints; performing intervention and treatment on different groups of rats after one week, wherein a treatment group adopts interceptive treatment in a way of DPSCs cell suspension liquid articular cavity orthotopic injection or intravenous injection; taking materials after treatment for 4 weeks and analyzing a treatment effect, and evaluating the treatment effect of DPSCs on the osteoarthritis of the knee joints. According to the method disclosed by the invention, an experimental result proves that the DPSCs has a treatment effect on bone osteoarthritis induced by combination of CFA and MIA; after treatment for 4weeks, through DPSCs systematic or local injection, a knee joint bone structure induced by the combination of CFA and MIA can be recovered, and exudates of the articular cavity are reduced. Through the DPSCs systematic or local injection, a transparent cartilage arrangement structure and cell forms can be recovered.

Description

technical field [0001] The invention belongs to the technical field of application of dental pulp mesenchymal stem cells (DPSCs) in osteoarthritis, and in particular relates to a method for treating knee osteoarthritis by blocking dental pulp mesenchymal stem cells. Background technique [0002] Primary knee osteoarthritis (Osteoarthritis of Knee, referred to as knee OA) is a common degenerative joint disease in middle-aged and elderly people, belonging to a chronic degenerative osteoarthropathy, with articular cartilage degeneration and peripheral joint Bone hyperplasia is a pathological feature, mainly manifested by knee joint pain, stiffness and limited mobility. The patients with this disease, from young to old, are in so much pain that it is difficult to move, and the quality of life cannot be guaranteed, which seriously troubles the healthy life of the patients. With the advancement of science and technology and the prolongation of human lifespan, the probability of o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/28A61P19/02A61P29/00
Inventor 李军
Owner 上海卡智生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap