Pulp capping agent with bioactivity and preparation method thereof
A bioactive, pulp capping technology, used in dental preparations, pharmaceutical formulations, pharmaceutical sciences, etc.
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[0032] In this example, the teeth used are the undisturbed premolars that need to be extracted due to the design of the orthodontic treatment plan, or the extracted healthy impacted teeth, supernumerary teeth, and deciduous teeth.
[0033] The bioactive pulp capping agents listed in this example include dentin extract and dentin powder. The dentin extract is the supernatant obtained by culturing dentin tissue in α-MEM medium. The dentin powder mentioned above is obtained by grinding the dentin cultured in the α-MEM medium.
[0034] The preparation method of the bioactive pulp capping agent enumerated in the embodiments of the present invention is as follows:
[0035] (1) Soak the extracted tooth in PBS buffer solution and wash it 3 times, use the NSK dental low-speed handpiece at 20,000 rpm to amputate the crown and part of the apex, and then use the NSK high-speed handpiece at 300,000 rpm Remove the periodontal ligament and cementum, extract the pulp in the remaining tooth t...
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