Injectable tissue engineering building method for regenerating dental pulp dentin

A technology of tissue engineering and construction method, applied in medical science, prosthesis, etc., can solve problems such as poor resistance, low chewing efficiency, lack of tooth sensation, etc., and achieve the effect of promoting cell growth and differentiation

Inactive Publication Date: 2015-01-14
SHANGHAI TISSUE ENG LIFE SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biggest disadvantages of these treatment methods are the lack of tooth

Method used

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  • Injectable tissue engineering building method for regenerating dental pulp dentin
  • Injectable tissue engineering building method for regenerating dental pulp dentin
  • Injectable tissue engineering building method for regenerating dental pulp dentin

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Embodiment 1

[0057] An injectable tissue engineering construction method for pulp and dentin regeneration: construction of a composite of cells and injectable scaffold material injected subcutaneously in nude mice

[0058] 1, Preparation of Injectable Hyaluronic Acid Gel Scaffold Material

[0059] Drugs: hyaluronic acid (HA, Shandong Freda Biomedical Co., Ltd.); 1,4-butanediol glycidyl ether (BDDE, American Sigma Company); sodium hydroxide, sodium chloride, potassium dihydrogen phosphate, phosphoric acid Disodium hydrogen (Sinopharm Chemical Pharmaceutical Co., Ltd.).

[0060] Steps: Accurately weigh 0.5g HA and place it in 5ml of 1% sodium hydroxide solution until completely dissolved. Add 20ml BDDE to the HA solution, spread the solution on a petri dish, and cross-link for 3 days at room temperature;

[0061] Then add an appropriate amount of phosphate buffer saline (PBS: sodium chloride (NaCl) 9mg / ml, potassium dihydrogen phosphate (KH 2 PO 4 ) 0.03 mg / ml, disodium hydrogen phosph...

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Abstract

The invention discloses an injectable tissue engineering building method for regenerating dental pulp dentin. The method comprises the following three steps: preparation of an injectable hyaluronic acid gel scaffold material, separation and cultivation of mesenchymal cells of tooth germs, building of cells and injectable scaffold material compounds in different microenvironments, material preparation of implants and histological detection. According to the method disclosed by the invention, the third generation of mesenchymal cells of tooth germs are seed cells for regenerating dentin pulp sample structures by using a tissue engineering method; TGF-beta1 plays a crucial role in building of the dentin pulp sample structures; meanwhile, the hyaluronic acid gel can be an injectable scaffold material and is suitable for injectable dental tissue engineering; the third generation of mesenchymal cells of tooth germs, the TGF-beta1 and the hyaluronic acid gel are combined and injected into an emedullated dental pulp cavity to build the injectable tissue engineering; thus, in situ regeneration of a dentin-pulp complex sample structure can be realized under the micro-trauma condition, and a new possibility is provided for in situ and physiological regeneration of the dentin-pulp complex in clinic.

Description

technical field [0001] The invention relates to an injectable tissue engineering construction method for pulp and dentin regeneration, and belongs to the field of recreating biologically active structures by using engineering methods. Background technique [0002] For a long time, tooth damage has brought a lot of distress to people of all ages, seriously affecting people's chewing, aesthetics and psychology. Clinically, severely damaged teeth often need to be pulped. As a result, the tooth loses its activity and resistance to external stimuli. It is likely that it will need to be repaired again, and eventually the entire tooth may need to be extracted. At present, the routine treatment methods for teeth after pulp removal are mainly dental material filling and denture restoration. However, the biggest disadvantages of these treatment methods are that the teeth lack sensation, the chewing efficiency is not high, the resistance is poor, and the retention is difficult. [00...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/38A61L27/54
Inventor 岑莲曹谊林谭林花尹烁
Owner SHANGHAI TISSUE ENG LIFE SCI
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