Preparation method of super-bionic soft and hard tissue composite scaffold

Abstract

The invention discloses a preparation method of a super-bionic soft and hard tissue composite scaffold. The preparation method comprises the following steps: designing a bionic bone tissue scaffold, preparing a printing raw material, preparing printing ink, performing DLP photocuring 3D printing, performing freeze drying, extracting dental pulp mesenchymal stem cells of a patient, performing directional differentiation, inoculating and culturing the cells, preparing a soft tissue scaffold and preparing a soft and hard tissue composite scaffold. Through 3D printing of the super-bionic biological material, simultaneous personalized repair of oral clinical soft and hard tissues is achieved, development of a second operation area is avoided, and the treatment time for stage repair of the soft and hard tissues at present is shortened. Meanwhile, the three-dimensional microstructure of the jawbone tissue and the Harvard tube and the Walkman tube simulating the jawbone are inspired to establish good blood supply in the early stage, conditions are provided for layered induction of the bionic jawbone tissue, and a new thought is provided for design, research and development of the bionic scaffold.

Description

technical field [0001] The invention belongs to the preparation method of a bionic support in the field of tissue engineering, and in particular relates to a preparation method of a super bionic soft and hard tissue composite support. Background technique [0002] Generally speaking, bone tissue has a unique self-regeneration and healing ability, but when the bone defect exceeds the limit range (>5-8mm), its self-healing ability is often unsatisfactory. However, clinically, oral bone tissue defects, especially large-scale alveolar bone insufficiency, will directly affect the patient's oral restorative treatment, that is, the reconstruction of masticatory function and the restoration of aesthetics. In particular, implant dentures have become the most mainstream treatment method in oral restoration at present, and their requirements for alveolar bone volume are quite high. If the bone volume is insufficient, the difficulty of implant surgery will be greatly increased, and e...

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Problems solved by technology

In addition, since the jaw needs to transmit force through the occlusion of the teeth, and it needs to have a good adaptive reconstruction function under the action of the occlusal force, this has a more unique understanding of the structure and connection of the trabecular bone in the cancellous bone. However, the current biomaterials used to repair the jaw rarely emphasize its functional properties and only focus on its morphological reconstruction. Therefore, the special bone tissue engineering scaffold materials for jaw reconstruction in the oral cavity are designed and manufactured to adapt to the oral environment and environment. Function is the bottleneck of current research

If the graft material can fully imitate the vascular structure of natural jaw bone tissue, while ensuring the blood supply and loading the patient's own source of multi-potential stem cells, it will not only induce the new bone tissue to perform the physiological functions it should undertake in the mouth, but will also There is an immunogenic risk brought by heterologous cells, but no scholars have simulated the microstructure of natural jaw bone to prepare larger-sized jaw bone scaffold materials from the perspective of microcosm and clinical transformation

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