Preparation technology of novel nerve conduit

A preparation process and nerve conduit technology, which is applied in the field of biological nerve tissue engineering, can solve the problems of limited donor nerve materials and limited wound pain, and achieve good degradability, restoration of structure and function, and low cytotoxicity

Inactive Publication Date: 2016-03-23
JIANGSU PROVINCE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the availability of donor nerves is limited, and postoperative wound pain, loss of function, and scar formation in the donor area limit the application of autologous transplantation

Method used

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  • Preparation technology of novel nerve conduit

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Experimental program
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Effect test

Embodiment 1

[0047] A preparation process of a new type of nerve guide, which sequentially includes the preparation process of GDNF-loaded gelatin microspheres, the synthesis process of gelatin-methacrylamide hydrogel, the preparation method of polycaprolactone / gelatin electrospun nerve tube and the electrospun Binding method of spun neural tubes and GDNF-loaded gelatin microspheres.

[0048] The preparation process of the preparation of the GDNF-loaded gelatin microspheres specifically includes the following steps:

[0049] A1. Prepare a type B gelatin aqueous solution with a mass volume fraction of 10%, and preheat it to 45°C for later use;

[0050] A2. Put the liquid paraffin containing 1% span80 in a water bath at 45°C for 20 minutes, slowly drop in the above 10ml gelatin aqueous solution, and stir magnetically for 15 minutes in a water bath at 45°C to form an emulsion;

[0051] A3. Put the emulsion obtained in step A2 through an ice bath to quickly cool down the emulsion to 4°C, cont...

Embodiment 2

[0076] The difference with Example 1 is that the 10 μl concentration described in step A7 is 8 μg / ml, and the pH value of the GDNF solution is 7.0; Ammonium persulfate and tetramethylethylenediamine in the same volume were sequentially added to the methacrylamide hydrogel solution at a concentration of 600 mM.

Embodiment 3

[0078] The difference with Example 1 is that the 10 μl concentration described in step A7 is 8 μg / ml, and the pH value of the GDNF solution is 7.5; Ammonium persulfate and tetramethylethylenediamine in the same volume were sequentially added to the methacrylamide hydrogel solution, both at a concentration of 1100 mM.

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Abstract

The invention discloses a preparation technology of a novel nerve conduit, and belongs to the technical field of tents of biological tissue engineering. The preparation technology sequentially comprises a preparation technology of GDNF (glial-derived neurotrophic factor)-carried gelatin microspheres, a synthesizing technology of gelatin-methacrylamide hydrogel, a preparation method of polycaprolactone/gelatin electricity texture nerve conduits, and a combining method of the electricity texture nerve conduits and the GDNF-carried gelatin microspheres. The preparation technology has the advantages that a tent structure with good degradability, biological compatibility and biological safety is realized, and the GDNF is controlled and released by the GDNF-carried gelatin microspheres; compared with blank groups and collagen conduit groups, the regeneration of peripheral nerves is effectively promoted, and the structure and function of restoring damaged nerves can reach the effect similar to the effect of autotransplantation groups; the defect nerves can be bridged, and the stability of axon regeneration environment can be maintained; the invasion of peripheral connective tissues is prevented; the saturing of near and far-heart breaking ends of smaller nerve defect is solved, and the restoration of larger nerve defect is solved.

Description

technical field [0001] The invention relates to the technical field of biological nerve tissue engineering, in particular to a preparation process of a novel nerve guide. Background technique [0002] Peripheral nerve (PNS) injury caused by trauma or tumor often leads to permanent loss of target organ function and nerve pain, which significantly reduces the quality of life of patients. Therefore, how to repair the defective nerve and restore the function of the target organ has always been a difficult and hotspot of research. At present, there are mainly the following difficulties in the repair of peripheral nerve injury: ① how to construct a microenvironment suitable for nerve regeneration; ② how to reduce the apoptosis of neurons and the atrophy of target organs in the process of nerve regeneration. Clinically, smaller nerve defects are usually repaired by suturing the epineurium at the proximal and distal stumps to achieve better results. However, when the nerve defect ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/58
CPCA61L27/22A61L27/50A61L27/58A61L2430/32C08L89/00C08L33/06
Inventor 庄海卜寿山许金菊张继生宋冬生
Owner JIANGSU PROVINCE HOSPITAL
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