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Quantum dot fluorescence detection method for folic acid in red blood cells

A Fluorescence Detection, Quantum Dot Technology

Active Publication Date: 2021-12-24
山东子峰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the microbial detection method is cumbersome to operate, and the detection results are affected by the sensitivity of microorganisms, antifolate drugs or antibiotic components contained in the sample, and the detection results lack repeatability
Radioimmunoassay technology has the advantages of being fast and simple, but during the production of reagents, isotopes are used to label active proteins, so only a few manufacturers can produce corresponding reagents, and the testing equipment is expensive; and different binding proteins respond to folic acid subtypes Sensitivity is different, resulting in a large difference in test results between kits, so the reliability of its application in clinical testing is not high; the main active ingredient of related reagents is protein products, and changes in external temperature can easily lead to inactivation of detection reagents, so Competitive radioimmunoassay products have high requirements for production, storage and transportation environment, which increases the economic cost of the product and is difficult to promote in primary hospitals

Method used

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  • Quantum dot fluorescence detection method for folic acid in red blood cells
  • Quantum dot fluorescence detection method for folic acid in red blood cells
  • Quantum dot fluorescence detection method for folic acid in red blood cells

Examples

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preparation example Construction

[0055] Specifically, the preparation method includes:

[0056] Add CdSe / ZnS quantum dots to MOPS buffer for activation, then add EDC and NHS to it, and then add folic acid monoclonal antibody to react for 2~4 hours. After completion, use borate buffer solution for elution and centrifugation, reconstitute and dilute.

[0057] Wherein, the MOPS buffer is neutral or weakly acidic, preferably with a pH of 6.0-7.4 and an ionic strength of 20-100 mM.

[0058] The blocking agent is a polyhydroxy compound, preferably glucosamine.

[0059] The borate buffer solution is weakly alkaline, more preferably pH 8-9, and ionic strength 20-100mM.

[0060] The reaction membrane is a nitrocellulose membrane (NC membrane), and a hemoglobin interception line, a detection line, and a quality control line are set according to the chromatography direction; the hemoglobin interception line is fixed with a specific adsorption substance of hemoglobin, and the detection line is fixed with folic acid Th...

Embodiment 1

[0074] This embodiment provides a detection method for detecting red blood cell folic acid based on fluorescent quantum dots, including detecting test strips such as figure 1 As shown, the test strip includes a bottom plate 9 and a chromatographic structure, and the bottom plate 9 carries the chromatographic structure.

[0075] The chromatographic structure has a filter membrane 1, a sample pad 2, a binding pad 3, a reaction membrane 8 and an absorbent paper 7 from the sample loading end to the sample chromatography direction, and the filter membrane 1, sample pad 2, binding pad 3, reaction The membranes 8 are overlapped sequentially, and the part of the absorbent paper 7 that touches the reaction membrane 8 is overlapped on the reaction membrane 8 .

[0076] In this embodiment, the quantum dot fluorescence detection method of folic acid in red blood cells using the above-mentioned detection test strip, the specific operation steps are as follows:

[0077] 1) Preparation of c...

Embodiment 2

[0091] In this embodiment, another test strip for detecting folic acid based on fluorescent quantum dots is provided. The difference from Embodiment 1 is that in this embodiment, the filter membrane is a cellulose acetate membrane, and the hemoglobin interception line 4 The streaked concentration is 0.8mg / mL anti-hemoglobin monoclonal antibody, and the streaked concentration of detection line 5 is 1.0mg folic acid-BSA;

[0092] The length of the portion of the filter membrane 1 covering the sample pad 2 is 1.3 cm;

[0093] The length of the part of the sample pad 2 covering the bonding pad 3 is 1.8 mm;

[0094] The length of the part of the bonding pad 3 covering the reaction membrane 8 is 1.8 mm;

[0095] The length of the portion of the absorbing paper 7 covering the reaction membrane 8 is 1.8 mm.

[0096] The distance between the hemoglobin interception line 4 and the upper edge of the NC membrane 8 is 5 mm, the distance from the detection line 5 is 4 mm, and the distance...

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Abstract

The invention relates to a quantum dot fluorescence detection method for folic acid in red blood cells. Folic acid is an essential vitamin in the growth and development process of a human body, and has the effects of preventing neonatal neural tube deformity, reducing anemia and preeclampsia of pregnant women and the like. Therefore, the detection method which is simple and convenient to operate, sensitive and reliable is provided and has important significance for monitoring the folic acid supplement effect of people such as pregnant women. The invention provides the quantum dot fluorescence detection method for folic acid in red blood cells, in order to improve the detection accuracy, pretreatment and dissociation steps for detection are optimized, and full rupture and dissociation of folic acid in red blood cells are realized. The invention further provides a solid-phase chromatography test strip based on quantum dot detection, folic acid detection based on the test strip has good precision and sensitivity, and the test strip can adapt to normal-temperature transportation and has an ideal market prospect.

Description

technical field [0001] The invention belongs to the technical field of blood marker detection, and in particular relates to a quantum dot fluorescence detection method of folic acid in red blood cells. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Folic acid is a water-soluble B vitamin composed of pteridine, p-aminobenzoic acid and L-glutamic acid. Folic acid, also known as pteroyl glutamic acid, is a vitamin substance essential for the synthesis of nucleic acids, thymidylic acid, neurotransmitters, phospholipids, and hormones. It acts as a carrier of one-carbon units in the body and participates in various metabolic reactions. Folate is also a component of the essent...

Claims

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Application Information

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IPC IPC(8): G01N33/82G01N33/577G01N33/558G01N33/58G01N33/53G01N33/532G01N33/52G01N21/64
CPCG01N33/82G01N33/577G01N33/558G01N33/588G01N33/5306G01N33/532G01N33/52G01N21/6428
Inventor 何长锋谢煜萍姚丛丛王学士
Owner 山东子峰生物技术有限公司
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