Method of screening fetal open neural tube defects with maternal serum alpha-fetoprotein heterogeneity l2 and l3 in the second trimester

Abstract

The invention discloses a method for screening fetal open neural tube defects (ONTD) by maternal serum alpha-fetoprotein heterogeneity L2 and L3 in the second trimester. The method comprises the following steps: (1) ONTD is confirmed by ultrasound imaging A number of pregnant women with fetuses formed the case group, and multiple pregnant women with normal fetal development were randomly selected to form the control group; (2), the levels of AFP-L2 and AFP-L3 in the serum of two groups of pregnant women were detected by enzyme-linked immunosorbent assay, and the levels of AFP-L2 and AFP-L3 in the second trimester The correlation between AFP-L2 and AFP-L3 levels in women's serum and fetal ONTD was statistically processed, and the screening efficiency of different risk calculation models was analyzed; (3), AFP-L2 and AFP were calculated according to the ROC curve ‑L3 optimal cut-off value and area under the curve for screening ONTD fetuses. The beneficial effect of the present invention is: the screening ONTD fetus of middle pregnancy maternal serum AFP-L2 and AFP-L3 has higher sensitivity and specificity, is a better marker for screening ONTD, using AFP-L2 and AFP-L3 The risk calculation model constructed jointly has better screening efficiency than using AFP, AFP‑L2, and AFP‑L3 alone to screen for MoM values.

Description

technical field [0001] The invention relates to the field of medical detection, and mainly relates to a method for screening fetal open neural tube defects by maternal serum alpha-fetoprotein heterogeneity L2 and L3 in the second trimester. Background technique [0002] Neural tube defects (NTD) is a serious congenital birth defect disease caused by neural tube insufficiency during embryogenesis. It is the result of the combined effects of environment and genetics. 1‰ [1] . According to whether the affected nerve tissue is exposed to the body surface, open neural tube defects (ONTD) and closed neural tube defects are divided. ONTD is more common, including open spina bifida, anencephaly, and encephalocele. Clinical manifestations vary according to the location and severity of the defect [2-3] . Currently, the second trimester (15-20 +6 Zhou) Serological ONTD prenatal screening, mainly to screen three types of ONTD: open spina bifida, anencephaly and encephalocele, throu...

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Problems solved by technology

[0003] In the previous work of this study, the screening efficiency of AFP screening for ONTD was not ideal, and the second-trimester maternal serum dual method (AFP and freeβ-HCG) used Risks 2T birth screening analysis software to screen fetal anencephaly, spina bifida, and encephalocele. The detection rate was 52.63% (10 / 19), 53.33% (32 / 60), 39.13% (9 / 23) [7] ; while the duplex method used LifeCycle 4.0 prenatal screening analysis software to screen ONTD in 46 089 cases and 180 006 cases of pregnant women, and the screening sensitivities were 0 [18] and 38.46% [19]

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