The invention relates to an oxidase
response type molecular probe as well as a preparation method and application thereof, and belongs to the technical field of
medical imaging. Aiming at the problem that the
inflammation activity is difficult to accurately evaluate in the prior art, the invention provides a
molecular imaging probe capable of responding to the activity of in-vivo
myeloperoxidase (MPO), and the
molecular imaging probe can be used for real-time and non-invasive imaging monitoring of the activity of the in-vivo MPO, so that the activity of the inflammatory reaction of a focus is accurately evaluated. The invention provides a chelating agent with structural characteristics of a chelating unit and an
enzyme response unit, and is characterized in that the
enzyme response
molecular probe is constructed by taking rigid framework cyclohexanediamine (CDTA) as a
transition metal chelating unit and taking
electron-rich functional groups (such as
phenol,
catechol or 5-hydroxytryptamine) as the
enzyme response unit. The probe is chelated with paramagnetic
metal (such as Mn < 2 + >) for
magnetic resonance imaging (MRI) or radioactive
metal (such as 68Ga) for
positron emission
tomography (PET), so that specific response to MPO activity is realized. The dynamic stability of the probe is remarkably improved; after the MPO is responded, the relaxation efficiency is improved by 2-3 times, and the sensitivity is optimized; the
cytotoxicity is low; in an
acute pancreatitis model, the MRI contrast
noise ratio (
delta CNR) reaches 77.39 + / -7.04 and is 4.2 times that of a control group, the PET
uptake rate is remarkably improved, bimodal imaging is supported, and accurate positioning and quantitative evaluation of the
inflammation activity are achieved.