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Creation method of giant flower pop corn

A technology of popping corn and giant, which is applied in botany equipment and methods, angiosperms/flowering plants, plant genetic improvement, etc. It can solve the problems of slow genetic gain of expansion coefficient, increase of popcorn volume, lack of large flower resources, etc. Achieve the effects of human, animal and environmental safety, low cost, and simple breeding process

Active Publication Date: 2020-12-11
SHENYANG TEYIJIA CORN TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014]Aiming at the lack of large flower resources in large flower breeding of popcorn, slow genetic gain of expansion coefficient, long breeding cycle, poor effect and high cost, this paper The invention proposes a method for creating giant flower popcorn, referring to conventional corn chromosome doubling technology, and creating large grain and large flower popcorn through the ploidy breeding approach of autotetraploid, using germplasm with large flower and high expansion coefficient as the test material, That is, on the basis of large flowers, the volume of corn flowers is increased again and greatly, and a new variety of popcorn with giant flowers is bred, which can increase yield and reduce the rate of dregs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056]1. Seed selection and germination. The popped corn inbred lines 'TQ10' and 'TQ5' with large grain size, large flower type and high popping coefficient were selected. The 'TQ10' and 'TQ5' were the parents of the popped corn of 'Jiaqiu 105'. incubator germination;

[0057] The seed size of the popped corn inbred line is 5.5 mm; the single flower volume is 6.0 mL; the spherical flower has a 25-fold expansion coefficient;

[0058] Use the Petri dish test paper method to promote germination, lay 4 layers of test paper in the Petri dish, soak the test paper without leaving the water and reach saturation, put the seeds evenly on the test paper, cover the lid and place it at 25 ℃ constant temperature incubation Germinate in the box.

[0059] 2. The buds germinate. When the buds grow to 2-3mm, transfer the germinated seeds from the germination petri dish to the liquid petri dish, replace the water with the chromosome doubling agent, and keep other things unchanged, and place t...

Embodiment 2

[0075] 1. Seed selection and germination. Select popcorn inbred lines 'TD11' and 'TD7' with large grain shape, large flower shape and high expansion coefficient, that is, the parents of 'Jiadie 117', and germinate in a petri dish in an incubator;

[0076] The popped corn inbred seed has a grain size of 5.0 mm; a single flower volume of 7.0 mL; butterfly-shaped flowers, with an expansion coefficient of 35 times;

[0077] Use the Petri dish test paper method to promote germination, lay 4 layers of test paper in the Petri dish, soak the test paper without leaving the water and reach saturation, put the seeds evenly on the test paper, cover the lid and place it at 25 ℃ constant temperature incubation Germinate in the box.

[0078] 2. The buds germinate. When the buds grow to 2-3mm, transfer the germinated seeds from the germination petri dish to the liquid petri dish, replace the water with the chromosome doubling agent, and keep other things unchanged, and place them in a 16-17...

Embodiment 3

[0094] 1. Seed selection and germination. The popped corn inbred lines 'Q26' and 'Q534' with large grain shape, large flower shape and high expansion coefficient, that is, the parents of 'Jiaqiu 26', were selected for germination in a petri dish in an incubator;

[0095] The popped corn inbred seed has a grain size of 5.5 mm; a single flower volume of 6.0 mL; a mixed flower, with an expansion coefficient of 30 times;

[0096] Use the Petri dish test paper method to promote germination, lay 4 layers of test paper in the Petri dish, soak the test paper without leaving the water and reach saturation, put the seeds evenly on the test paper, cover the lid and place it at 25 ℃ constant temperature incubation Germinate in the box.

[0097] 2. The buds germinate. When the buds grow to 2-3mm, transfer the germinated seeds from the germination petri dish to the liquid petri dish, replace the water with the chromosome doubling agent, and keep other things unchanged, and place them in a...

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Abstract

The invention relates to a creation method of giant flower pop corn. The method comprises the following steps of (1) selecting seeds and accelerating germination, namely selecting pop corn selfing line seeds with the large grain size, the large flower size and the high expansion coefficient, and accelerating germination for the first time; (2) performing doubling treatment, namely enabling a chromosome doubling treatment agent to be any one of amiprophos-methyl, trifluralin and colchicine of herbicide; (3) performing breeding; (4) performing phenotype screening and fructification identification; and (5) performing combination and preparation, namely, adopting doubled selfing line seeds as parents, and performing hybridization and matching to obtain a high-yield and high-expansion-coefficient pop corn hybrid. According to the method, a conventional corn chromosome doubling technology is used for reference, large-grain large-flower pop corn is created through a ploidy breeding approach of autotetraploid, large-flower and high-expansion-coefficient germplasm is used as a test material, namely, on the basis of large flowers, the size of the corn flowers is greatly increased again, a giant flower pop corn new variety is bred, and the yield can be increased.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for producing popcorn with giant flowers. Background technique [0002] Expansion performance ranks first in the quality requirements of popping corn. The indicators to measure the expansion performance mainly include explosion rate, expansion multiple, expansion coefficient and single flower volume, etc. Some of the indicators are greatly affected by environmental factors, and some mainly depend on internal factors. Among the four indicators, the expansion coefficient is the core indicator, which is affected by the internal chemical and physical factors of the endosperm. The expansion coefficient is expressed by the ratio of the volume of each corn kernel before and after popping (the volume of corn kernels / the volume of corn kernels), so as to illustrate the degree of expansion of corn kernels. [0003] Larger popcorn is a constant quest not only f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H1/04A01H1/02A01H5/10A01H6/46
CPCA01H1/08A01H1/04A01H1/02A01H5/10A01H6/4684Y02A40/10
Inventor 史振声孙淑凤张喜华王莹
Owner SHENYANG TEYIJIA CORN TECH
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