Creation method of giant flower pop corn
A technology of popping corn and giant, which is applied in botany equipment and methods, angiosperms/flowering plants, plant genetic improvement, etc. It can solve the problems of slow genetic gain of expansion coefficient, increase of popcorn volume, lack of large flower resources, etc. Achieve the effects of human, animal and environmental safety, low cost, and simple breeding process
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Embodiment 1
[0056]1. Seed selection and germination. The popped corn inbred lines 'TQ10' and 'TQ5' with large grain size, large flower type and high popping coefficient were selected. The 'TQ10' and 'TQ5' were the parents of the popped corn of 'Jiaqiu 105'. incubator germination;
[0057] The seed size of the popped corn inbred line is 5.5 mm; the single flower volume is 6.0 mL; the spherical flower has a 25-fold expansion coefficient;
[0058] Use the Petri dish test paper method to promote germination, lay 4 layers of test paper in the Petri dish, soak the test paper without leaving the water and reach saturation, put the seeds evenly on the test paper, cover the lid and place it at 25 ℃ constant temperature incubation Germinate in the box.
[0059] 2. The buds germinate. When the buds grow to 2-3mm, transfer the germinated seeds from the germination petri dish to the liquid petri dish, replace the water with the chromosome doubling agent, and keep other things unchanged, and place t...
Embodiment 2
[0075] 1. Seed selection and germination. Select popcorn inbred lines 'TD11' and 'TD7' with large grain shape, large flower shape and high expansion coefficient, that is, the parents of 'Jiadie 117', and germinate in a petri dish in an incubator;
[0076] The popped corn inbred seed has a grain size of 5.0 mm; a single flower volume of 7.0 mL; butterfly-shaped flowers, with an expansion coefficient of 35 times;
[0077] Use the Petri dish test paper method to promote germination, lay 4 layers of test paper in the Petri dish, soak the test paper without leaving the water and reach saturation, put the seeds evenly on the test paper, cover the lid and place it at 25 ℃ constant temperature incubation Germinate in the box.
[0078] 2. The buds germinate. When the buds grow to 2-3mm, transfer the germinated seeds from the germination petri dish to the liquid petri dish, replace the water with the chromosome doubling agent, and keep other things unchanged, and place them in a 16-17...
Embodiment 3
[0094] 1. Seed selection and germination. The popped corn inbred lines 'Q26' and 'Q534' with large grain shape, large flower shape and high expansion coefficient, that is, the parents of 'Jiaqiu 26', were selected for germination in a petri dish in an incubator;
[0095] The popped corn inbred seed has a grain size of 5.5 mm; a single flower volume of 6.0 mL; a mixed flower, with an expansion coefficient of 30 times;
[0096] Use the Petri dish test paper method to promote germination, lay 4 layers of test paper in the Petri dish, soak the test paper without leaving the water and reach saturation, put the seeds evenly on the test paper, cover the lid and place it at 25 ℃ constant temperature incubation Germinate in the box.
[0097] 2. The buds germinate. When the buds grow to 2-3mm, transfer the germinated seeds from the germination petri dish to the liquid petri dish, replace the water with the chromosome doubling agent, and keep other things unchanged, and place them in a...
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