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A kind of method applicable to the rooting of tissue culture seedlings of color-leaved plants

A plant tissue culture and color leaf technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as restricting social and economic development, aggravating the contradiction between resource supply and economic development, and destroying natural environment and ecological resources. , to achieve the effect of improving callus differentiation rate, accelerating callus proliferation, and promoting cell division.

Active Publication Date: 2022-05-03
ANYANG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the construction and development of urban gardens, the green area has gradually expanded, but there are still a large number of extravagant and wasteful phenomena such as blind introduction of trees, transplanting of large trees, off-season planting, and adversity planting during the construction process, which makes the natural environment and ecological resources that the city relies on These phenomena run counter to the concept of a resource-saving society and sustainable development advocated under the special national conditions of our country with a large population and little land and relatively scarce resources. It not only intensifies the contradiction between resource supply and economic development, but also The lack of it also limits the development of social economy

Method used

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  • A kind of method applicable to the rooting of tissue culture seedlings of color-leaved plants

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Effect test

Embodiment 1

[0024] A method applicable to the rooting of tissue culture seedlings of color-leaved plants. The color-leaved plants select sweetgum sweetgum, including an induction culture process and a proliferation culture process, and also includes a rooting culture process; the rooting culture process is composed of the following steps:

[0025] 1) Cut the clustered buds that have been multiplied and cultured into stems with buds, and inoculate them into the stimulated root medium, and perform light culture and dark culture alternately for 36 hours to obtain stimulated root materials; the condition of light culture is 4 hours of light, and the light intensity is 1400lux , the temperature is 23-26°C, and the humidity is 80%; the dark culture conditions are dark culture for 8 hours, the temperature is 19-23°C, and the humidity is 80%;

[0026] 2) Transfer the stimulated root material into the rooting medium, and culture it in low light at a temperature of 24-26°C and a humidity of 90% for ...

Embodiment 2

[0032] A method applicable to the rooting of tissue cultured seedlings of color-leaf plants, where the color-leaf plant selects the flower-leaf brocade, including induction culture process and proliferation culture process, and also includes a rooting culture process; the rooting culture process is composed of the following steps:

[0033]1) Cut the clustered buds that have been multiplied and cultured into stems with buds, inoculate them into stimulated rooting medium, and perform light culture and dark culture alternately for 42 hours to obtain stimulated root materials; the condition of light culture is 4 hours of light, and the light intensity is 1500lux , the temperature is 23-26° C., and the humidity is 82%; the dark culture conditions are 8 hours of dark culture, the temperature is 19-23° C., and the humidity is 82%.

[0034] 2) Transfer the stimulated root material into the rooting medium, culture in low light at a temperature of 24-26°C and a humidity of 91% for 4 days...

Embodiment 3

[0040] A method applicable to the rooting of tissue culture seedlings of color-leaved plants, the color-leaved plants are selected heather, including induction culture process and proliferation culture process, and also includes rooting culture process; rooting culture process is composed of the following steps:

[0041] 1) Cut the clustered buds that have been multiplied and cultured into stems with buds, inoculate them into root-stimulated medium, and perform light culture and dark culture alternately for 48 hours to obtain stimulated root materials; the condition of light culture is 4 hours of light, and the light intensity is 1600lux , the temperature is 23-26° C., and the humidity is 86%; the dark culture conditions are 8 hours of dark culture, the temperature is 19-23° C., and the humidity is 86%.

[0042] 2) Transfer the stimulated root material into the rooting medium, culture in low light at 24-26°C and 93% humidity for 3 days; then culture in strong light at 26-28°C a...

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Abstract

The invention discloses a method for rooting of tissue culture seedlings of coleus plants, including an induction culture process, a proliferation culture process, and a rooting culture process. The rooting culture process is composed of the following steps: 1) Proliferation culture The clumps of buds are cut into stem segments with buds, and inoculated into the rooting medium to stimulate the root material; 2) The stimulated root material is transferred to the rooting medium, and cultivated in low light; 3) The seedling cultivation was carried out in the dark, and then the seedlings were raised. The light time was 12h per day, and the nutrient solution was supplemented every seven days during the seedling raising. The invention prepares stimulating rooting medium and rooting medium by improving MS medium, adjusts the culture conditions and other supporting measures, and has the characteristics of high rooting rate, fast reproduction speed, high survival rate of transplanted seedlings, low cost, etc., and the cultivated seedlings are neat and tidy. Robust and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of tissue culture seedling rooting, and in particular relates to a method suitable for rooting tissue culture seedlings of color-leaved plants. Background technique [0002] As a natural material with life forms, plants can create a variety of space atmospheres in the garden, enrich the garden space, and make people feel beautiful. With the construction and development of urban gardens, the green area has gradually expanded, but there are still a large number of extravagant and wasteful phenomena such as blind introduction of trees, transplanting of large trees, off-season planting, and adversity planting during the construction process, which makes the natural environment and ecological resources that the city relies on These phenomena run counter to the concept of a resource-saving society and sustainable development advocated under the special national conditions of our country with a large population and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 郜新强石慧刘彦珍何玲敏蔡新
Owner ANYANG INST OF TECH
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