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Method for extracting effective component of coleus forskohlii

A technology for Coleus forskohlii and effective components, which is applied in the field of extracting the effective components of Coleus forskolin and isoforskolin, the effective component of Coleus forskohlii, can solve complex equipment, large investment, and undisclosed differences. Forskolin purity and other issues, to achieve the effect of environmental protection and high purity

Inactive Publication Date: 2013-07-03
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The related technologies of extracting Iveskoline existing at present mainly include: (1) Adopt techniques such as aqueous ethanol extraction, organic solvent extraction and silica gel column chromatography to prepare Iversokoline, there are following defects in this kind of technology: Use toxic organic solvents such as a large amount of chloroform, dichloromethane, sherwood oil or acetone, and the separating material silica gel (Chinese Patent Publication No. CN 1539493A and CN 101259123A) that are not easy to regenerate and reuse; (2) supercritical fluid extraction method, the method equipment Complicated, large investment, also undisclosed the purity of ifforskolin obtained by this method (Chinese Patent Publication No. CN 1539493A); (3) using aqueous ethanol extraction, macroporous resin purification, this method is simple and convenient, suitable for large-scale production, But the content of isofaskolin in the obtained extract is only 8% and 20% (Chinese patent ZL200410084646.9)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) 5 kg of the whole Coleus forskohlii herb, pulverize, pass through a 20-mesh sieve, heat extract 3 times with 60 liters of 70% ethanol, extract for 2 hours for the first time, extract 1 hour for the second and third times, combine the extracts for 3 times, Concentrate to about 8 liters. Dilute the concentrated solution to 25 liters with water, add 5 liters of 1% chitosan solution under low-speed stirring, let stand for 1 hour, filter, and adjust the filtrate to pH=8;

[0026] (2) The above-mentioned filtrate passes through 10 liters of HP20 type macroporous resin column with the flow velocity of 2BV / h (BV is the column bed volume of macroporous resin), removes impurity with 5BV water and 4BV 30% ethanol leaching successively, then washes with 4BV 85 % ethanol was used to elute, and the eluate was collected and concentrated to dryness. The concentrated solution was diluted to 2 liters with 20% ethanol, passed through a 2 liter HP20 macroporous resin column, the above...

Embodiment 2

[0031] (1) 5 kg of the whole Coleus forskohlii herb, crushed, passed through a 20-mesh sieve, soaked in 80% ethanol for 12 hours, and then percolated, collected 40 liters of percolation liquid, and concentrated to about 5 liters. The concentrated solution was diluted with water to 20 liters, filtered, and the filtrate was adjusted to pH=9;

[0032] (2) The above-mentioned filtrate passes through 10 liters of HZ818 type macroporous resin column with the flow velocity of 2BV / h (BV is the column bed volume of macroporous resin), removes impurity with 5BV water and 4BV 25% ethanol rinse successively, then with 4BV 80 % ethanol was used to elute, and the eluate was collected and concentrated to dryness. The concentrated solution was diluted to 2 liters with 20% ethanol, passed through 2 liters of HZ818 macroporous resin column, the above elution procedure was repeated, and the eluate was concentrated to dryness to obtain a macroporous resin extract;

[0033] (3) After dissolving t...

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Abstract

The invention belongs to the pharmaceutical field of traditional Chinese medicines, relates to a method for extracting an effective component of coleus forskohlii, and specifically relates to a method for extracting an effective component-isoforskolin of coleus forskohlii. The method comprises the steps of smashing whole herbs or stems of the coleus forskohlii, extracting with hydrous ethanol, and purifying by methods of macroporous resin column chromatography, octadecyl-bonded silica gel column chromatography, recrystallization, etc., thus obtaining isoforskolin with a purity higher than 90%. The method is environment-friendly in preparation process and is suitable for large scale production, and the prepared isoforskolin has purity as high as 90% and even 98% and can be used as a raw material for medicine development and a functional additive for health-care products.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a method for extracting effective components of Coleus forskohlii. Specifically, a method for extracting isofeskolin, an active ingredient of Coleus forskohlii. Background technique [0002] Coleus forskohlii (Willd) Briq. is a plant of the Labiatae genus Coleus Lour. in the family Labiatae, and can be used to treat diseases such as cardiovascular diseases, colds, coughs and respiratory disorders. Isoforskolin (isoforskolin) is the main active ingredient of domestic Coleus forskolin, and the prior art discloses that it has the effects of lowering blood pressure, lowering intraocular pressure, relieving asthma, anti-platelet aggregation, and anti-lung injury. The compound has multiple biological activities and has good application prospects in the development of medicine and health food. [0003] The related technologies of extracting Iveskoline existing at pre...

Claims

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Application Information

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IPC IPC(8): C07D311/92
Inventor 黄建明田璐璐汪亚勤殷嘉珺刘真君谢道涛
Owner FUDAN UNIV
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