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Method for measuring labdane diterpenoidal in coleus forskohlii

A technology of Coleus forskohlii and diterpenes, which is applied in the field of quality analysis of natural medicines, can solve the problems of time-consuming and complicated sample pretreatment process, save time, reduce the influence of extraction efficiency and method reproducibility, and simplify steps Effect

Inactive Publication Date: 2013-05-29
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects and deficiencies of the prior art, to provide a method for measuring the helioane type diterpenoids in Coleus forskohlii, in particular to a method for detecting diterpenoids in Coleus forskohlii by using matrix solid-phase dispersion technology. A method for helianthane-type diterpenes; this method solves the complicated and time-consuming problem of sample pretreatment before liquid chromatography analysis

Method used

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  • Method for measuring labdane diterpenoidal in coleus forskohlii
  • Method for measuring labdane diterpenoidal in coleus forskohlii

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Embodiment 1

[0035] The dried whole herb of Coleus forskohlii was pretreated by the matrix solid-phase dispersion method of the present invention, and then five kinds of helicanoid diterpenes were identified by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS), It includes:

[0036] (1) Sample pretreatment

[0037] Accurately weigh 0.10g of Coleus forskohlii herb powder (passed through a 60-mesh sieve), 0.5g of octadecyl-bonded silica gel, put it in a glass mortar, mix and grind for 3min, put the mixture into the bottom with a sieve plate Place the sieve plate on the top surface of the empty column tube, press the piston tightly, place the solid phase extraction column on the solid phase extraction device, first rinse it with 5mL of 10% methanol quickly, vacuum dry, and then use 8mL of methanol at about 2mL / The flow rate of min was eluted, the eluate was collected, blown dry with nitrogen in a water bath at 50°C, the residue was redissolved in 1 mL of methanol,...

Embodiment 2

[0042] The dried whole herb of Coleus forskohlii was pretreated by the matrix solid-phase dispersion method, and then a high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) was used to quantitatively determine the helicanoid diterpenes, which included the steps of:

[0043] (1) Sample pretreatment

[0044] Accurately weigh 0.25 g of Coleus forskohlii herb powder (passed through a 60-mesh sieve) and 1 g of octadecyl-bonded silica gel, put them in a glass mortar, mix and grind for 5 minutes. Put the mixture into an empty column tube with a sieve plate at the bottom, place a sieve plate on the top surface, and compact it with a piston. Place the solid-phase extraction column on the solid-phase extraction device, rinse it with 5mL of 5% methanol quickly, dry it in a vacuum, infiltrate with about 3mL of ethyl acetate: methanol (1:4), seal the piston at the lower end of the column, and ultrasonicate in a water bath. (45kHz) for 2min, then eluted ...

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Abstract

The invention belongs to the field of quality analysis for natural medicine, relates to a method for measuring labdane diterpenoidal in coleus forskohlii, and particularly relates to a method for measuring labdane diterpenoidal in coleus forskohlii by adopting a matrix solid-phase dispersion technology. The method disclosed by the invention comprises the following steps of: extracting diterpenoidal ingredients in coleus forskohlii by adopting a matrix solid-phase dispersion technology, and removing polar and nonpolar interferents; and then qualitatively and quantitatively analysing five labdane diterpenoidal such as isoforskolin by adopting a high-performance liquid chromatography method. According to the method disclosed by the invention, the complex process of sample pre-treatment is simplified, the time is saved, the consumption of an organic solvent is reduced, and the emulsion phenomenon caused by liquid-liquid extraction is eliminated; the detection method is accurate and sensitive; and a rapid and effective quality evaluation method is provided for coleus forskohlii.

Description

technical field [0001] The invention belongs to the field of quality analysis of natural medicines, and relates to a method for measuring the healane-type diterpenes in Coleus forskohlii, in particular to a method for detecting the healane-type diterpenes in Coleus forskohlii by using matrix solid-phase dispersion technology. Terpene method. Background technique [0002] Coleus forskohlii (Willd) Briq. is a plant of the Labiatae genus Coleus Lour. of the labiatae family. It has the functions of strengthening the heart, lowering blood pressure, antithrombotic, and relieving asthma. It has been used for the treatment of cardiovascular diseases and colds for a long time , cough and respiratory disorders. The prior art discloses that the main active ingredient in Coleus forskolin is helianthane type diterpene (also known as laudane type diterpene), including isoforskolin (isoforskolin), forskolin (forskolin) etc., the above components are usually used as the quality evaluation...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 黄建明殷嘉珺汪亚勤康云谭波田璐璐谢道涛
Owner FUDAN UNIV
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