38 results about "Platelet preservation" patented technology

A method for preserving blood platelets is described. The method uses 1%-3% gelatin in the platelet preservation medium, and storing the platelets in the preservation medium at temperatures below 0° C. and at least 70 atmospheres pressure for at least one day.

A blood bag system includes a BC pooling bag for centrifugation of a buffy coat, a filter for removing white blood cells from a supernatant liquid transferred from the BC pooling bag, a platelet preserving bag for reserving the supernatant liquid that has passed through the filter, a first tube connecting the BC pooling bag and an inlet of the filter, a second tube connecting the platelet preserving bag and an outlet of the filter, and a cassette to be fixed in the centrifugation and separation apparatus. The first tube and the second tube are disposed within the cassette. The cassette includes a first clamp section for closing and opening the first tube, and a second clamp section for closing and opening the second tube.

In a blood bag system mounted in a centrifugation and separation apparatus, a filter is settable in different appropriate orientations at storage time and usage time, and first and second tubes and a filter are arranged respectively in appropriate orientations during use. The blood bag system includes a BC pooling bag for centrifugation of buffy coat, the filter for removing white blood cells from a supernatant liquid transferred from the BC pooling bag, a platelet preserving bag for reserving the supernatant liquid that has passed through the filter, the first tube connecting the BC pooling bag and an inlet of the filter, the second tube connecting the platelet preserving bag and an outlet of the filter, a cassette fixable in the centrifugation and separation apparatus, an attachment for holding the filter, and a hinge section tiltable relative to the cassette with reference to an axis in the circumferential direction.

The invention discloses a medical PVC material with high oxygen permeability for platelet storage and a preparation method thereof. The material comprises the following components by weight: 100 parts of polyvinyl chloride resin, 45-60 parts of a plasticizer, 5-10 parts of a heat stabilizer, 0.1-2 parts of a lubricant, 0.1-0.5 part of a functional additive of sodium lactate. The invention has the advantages of reasonable formula, stable performance, simple process and low cost, effectively improves the oxygen permeability of platelet storage bag, prolongs the storage life of platelets, and is of great practical significance to the development of medical and health undertakings in our country and even the world.

The invention discloses an application of a protein kinase A activator in blood platelet storage and a blood platelet storage method. From the embodiment of the invention, the invention discloses an application of the protein kinase A activator in inhibiting blood platelet apoptosis or keeping the activity of blood platelets for the first time under circumstance that the inducing and adjusting mechanisms of blood platelet apoptosis are unknown. The protein kinase A activator can inhibit afunction induced by blood platelet apoptosis and can prevent the blood platelets from being affected by apoptosis in storage and pathological stress conditions, so that the blood platelet survival time is prolonged. The method is a novel policy of improving the survival period of the blood platelets. The result verifies that determination of service life and survival of the blood platelets by regulating apoptosis has far-reaching significance in prolonging the storage time of the blood platelets, increasing the clinical application efficiency of the blood platelets and preventing the stored blood platelets from being rejected due to afunction of the blood platelets.

In a blood bag system mounted in a centrifugation and separation apparatus, a filter is settable in different appropriate orientations at storage time and usage time, and first and second tubes and a filter are arranged respectively in appropriate orientations during use. The blood bag system includes a BC pooling bag for centrifugation of buffy coat, the filter for removing white blood cells from a supernatant liquid transferred from the BC pooling bag, a platelet preserving bag for reserving the supernatant liquid that has passed through the filter, the first tube connecting the BC pooling bag and an inlet of the filter, the second tube connecting the platelet preserving bag and an outlet of the filter, a cassette fixable in the centrifugation and separation apparatus, an attachment for holding the filter, and a hinge section tiltable relative to the cassette with reference to an axis in the circumferential direction.

The invention discloses a polyvinyl chloride material with high oxygen permeability and a preparation method thereof. The polyvinyl chloride material with high oxygen permeability comprises the following components in parts by weight: 60 parts of polyvinyl chloride resin with super-high molecular weight, 30-40 parts of polyvinyl chloride resin with middle molecular weight, 40-80 parts of plasticizer TOTM (Trioctyl Trimellitate), 5-20 parts of plasticizer DOA (Dioctyl Adipate), 0-20 parts of plasticizer DOS (Dioctyl Sebacate), 3-10 parts of secondary plasticizer and 0.5-5 parts of calcium and zinc thermal stabilizer. The basic resin used in the material disclosed by the invention is polyvinyl chloride resin which has high oil absorption and is prepared by a super-high molecular weight and middle molecular weight suspension method, the plasticizer TOTM with low mobility is selected, the cold-resistant plasticizers DOA and DOS are compounded in use, and epoxy plant oil plasticizer with the thermal stabilizing effect is used as an assistant, so that the low-temperature resistance and extraction resistance of the product are ensured, and the material has a high gas exchange rate and characteristic of high oxygen permeability and is suitable for blood storage, and particularly suitable for storage of blood platelets. The prepared PVC material with high oxygen permeability can make sure that blood platelets can be stored for 7 days at 22 DEG C, so that the material is suitable for preparation of blood platelet storage bags.

The invention discloses a medical shock storage device used for preservation of blood platelets, and relates to the technical field of medical equipment. The device comprises a storage box, a mountingplate is fixedly connected between two sides of the inner wall of the storage box, the bottom of the inner wall of the storage box is rotatingly connected with a transmission worm rod through a supporting plate, one end of the transmission worm rod runs through the supporting plate and extends to one side of the supporting plate, the position, located on one side of the supporting plate, of the bottom of the inner wall of the storage box is fixedly connected with a rotating electrical machine through a connecting block, and one end of the output shaft of the rotating electrical machine is fixedly connected with one end, extending to one side of the supporting plate, of the transmission worm rod through a coupling. The medical shock storage device used for preservation of the blood platelets provided by the invention greatly enhances the shock effect, realizes longer-lasting maintenance of activity of the blood platelets, achieves the purpose of stably shaking the blood platelets whileshocking the blood platelets, and realizes the purpose of greatly prolonging the time of biological activity of the blood platelets.

The invention belongs to the technical field of platelet antibody detection, and particularly relates to anti-screening cells suitable for platelet antibody detection and a preparation method and application thereof. The anti-screening cells are prepared by the following method of: 1) mixing three persons of O-type platelets with screened and confirmed antigen spectrums in an isovolumetric manner, cleaning with platelet cleaning solution, centrifuging, discarding supernatant, and retaining platelet precipitates; 2) resuspending the platelet precipitates in the step 1) by using platelet loading liquid, adjusting the cell concentration to (1.0-2.0)*10<10> / ml, and oscillating; and 3) cleaning again with the platelet cleaning solution after oscillation, resuspending the precipitates by using platelet preserving solution after cleaning, and adjusting the cell concentration to (2.0-5.0)*10<7> cells / ml to obtain the anti-screening cells suitable for platelet antibody detection. The platelet anti-screening cells prepared by the method can be applied to platelet antibody detection, and is long in preservation time, convenient to use and good in antigen preservation effect.

The invention relates to a platelet antibody detection method based on a nano material and a platelet preservation method. In the invention, nano composite particles prepared by wrapping PLGA with platelet membrane protein are applied to platelet antibody detection for the first time, which proves that the platelet antibody can be accurately detected by taking human-derived serum containing an anti-platelet antibody as a detection object, and good sensitivity is possessed. In the invention, improvement of clinical diagnosis of platelet immune abnormal diseases is facilitated, matched plateletsare provided for patients, a clinical infusion effect of the platelets is improved, clinical blood transfusion quality is increased, and precious blood resources are saved for the society at the sametime.

The present invention relates to a platelet additive solution (PAS) having an amount of one or more sialidase inhibitors and optionally one or more glycan-modifying agents; and one or more of PAS components that includes a salt, a citrate source, a carbon source, and any combination thereof. The present invention also relates to methods, compositions and kits for increasing the in vivo circulation time of isolated platelets by storing the platelets with one or more sialidase inhibitors. Additionally, the present invention relates to methods and compositions for reducing sialidase activity and inhibiting bacterial proliferation of one or more bacteria in a platelet product preparation from one or more donors.

The invention discloses a medical PVC material with high oxygen permeability for platelet storage and a preparation method thereof. The material comprises the following components by weight: 100 parts of polyvinyl chloride resin, 45-60 parts of a plasticizer, 5-10 parts of a heat stabilizer, 0.1-2 parts of a lubricant, 0.1-0.5 part of a functional additive of sodium lactate. The invention has the advantages of reasonable formula, stable performance, simple process and low cost, effectively improves the oxygen permeability of platelet storage bag, prolongs the storage life of platelets, and is of great practical significance to the development of medical and health undertakings in our country and even the world.

The invention discloses a platelet preservation method. According to the invention, the influence of different temperatures on the stored platelets is discussed from the aspects of apoptosis and activation of the stored platelets, loss of physiological functions of the platelets, hemostasis thrombus formation ability of the platelets returned to the body and removal efficiency, and finally, the optimal temperature for storing the platelets is selected and is higher than 4 DEG C and not higher than 8 DEG C, preferably 6 DEG C; the platelets stored at the temperature have the best physiological function, the best thrombosis ability and the slowest in-vivo clearing efficiency. Compared with the existing accepted preservation temperature of 4 DEG C and 22 DEG C, the preservation period of the platelets is prolonged, the physiological functions of the platelets are kept to the maximum extent, meanwhile, the requirements of simple and convenient platelet storage method, long-term mass preservation, convenient transportation and the like are met, and the method is suitable for clinical general popularization and application. Meanwhile, the mechanism of apoptosis of the platelets at different preservation temperatures is also researched, and the reasons of apoptosis caused by preservation of the platelets at low temperatures (4 DEG C and 6 DEG C) and 22 DEG C are obtained.

Provided is a method for producing platelets, in which damage to platelets is suppressed compared with a method in which platelets are separated using a filter from a megakaryocyte culture, and then the platelets are concentrated using a hollow fiber membrane and are further washed using the hollow fiber membrane, and purified platelets can be produced in a shorter period of time compared with the time that is taken to perform the above-described method so as to reduce damage to platelets. The method for producing purified platelets of the present invention includes a concentrating step of concentrating a megakaryocyte culture, and a centrifuging step of centrifuging platelets from an obtained concentrate.

The invention relates to the field of manufacturing of medical supplies, in particular to a platelet storage bag which comprises a bag body with the periphery fixedly connected to form a space in the center side for storing platelets, and a connecting pipe communicated with the interior of the bag body for circulation of the platelets. The bag body comprises two layers of isolating membranes which are in contact with blood platelets and only allow gas to pass through, oxygen generating layers and carbon dioxide absorbing layers which are compounded on the opposite surfaces of the two layers of isolating membranes in a one-to-one correspondence manner, calcium peroxide microcapsules are embedded in the oxygen generating layers, lithium hydroxide microcapsules are embedded in the carbon dioxide absorbing layers, and the oxygen generating layers and the carbon dioxide absorbing layers are compounded in a one-to-one correspondence manner. The oxygen generating layer can slowly release oxygen, the carbon dioxide absorbing layer can absorb carbon dioxide, oxygen filling and vacuumizing operation is not needed any more, platelets can be directly stored in the storage bag, use is convenient, and meanwhile the storage duration of the platelets can be guaranteed.

A system, apparatus, and method using carbon monoxide for treatment of platelets, and optionally other blood components and / or whole blood, allowing extended storage and increased viability of the stored blood platelets in vivo. The system allows an efficient use of the stored platelets by dividing the platelets into a population with a short residual lifetime for immediate use and a population that can remain in storage for longer. The storage apparatus enables safe platelet preservation and is equipped with an alarm to detect CO leakage.

The present invention relates to a platelet transmembrane potential detection method, which comprises: taking a washed platelet sample, carrying out re-suspending dilution with Hepes-Tyrodes buffer containing calcium chloride, adding to a flow tube, and recording as F; taking a washed platelet sample, carrying out re-suspending dilution with Hepes-Tyrodes buffer having 120-150 mmol / L of potassium chloride and 2-20 mmol / L of sodium chloride, adding to a flow tube, and recording as F0; adding gramicidin with a final concentration of 0.1-5 [mu]g / L to the F0 flow tube; adding DiBAC4(3) with a final concentration of 10-200 nmol / L to the F flow tube and the F0 flow tube, and dyeing; loading onto the machine and detecting; and calculating the result according to the corrected Nernst equation. According to the present invention, the method has characteristics of less platelet destruction, high sensitivity, good reproducibility, enrichment of the existing platelet storage quality assessment method, and prediction of the platelet storage damage.

Embodiments relate to an activated platelet preservation composition, a method for preserving activated platelet, and a preserved activated platelet using the same. According to at least one embodiment, there is provided an activated platelet preservation composition including a divalent cation, chloride, vitamin B, a selenium source and a solvent, a kit using the same, a method for preserving an activated platelet, and a preserved activated platelet using the same. According to various embodiments, it is possible to obtain and preserve platelets, which are positive for CD61 and CD62p and negative for PAC-1 among cell markers.

The invention discloses the application of protein kinase A activator in platelet preservation and the platelet preservation method. It can be seen from the examples of the present invention that the present invention discloses for the first time the application of protein kinase A activators in inhibiting platelet apoptosis or maintaining platelet activity when the induction and regulation mechanism of platelet apoptosis is still unknown. Protein kinase A Activators can inhibit the loss of function caused by platelet apoptosis, and can protect platelets from apoptosis under storage and pathological stress conditions, thereby prolonging the survival time of platelets. It is a new strategy to improve the survival period of stored platelets. The results show that the present invention determines the lifespan and survival of platelets by regulating apoptosis, prolongs the storage time of platelets, improves the clinical application efficiency of platelets, and avoids the inactivation of stored platelets due to the loss of platelet function. And being scrapped has far-reaching significance.

The invention provides blood platelet preserving fluid. A preparation method of the blood platelet preserving fluid comprises the following steps: preparing dimethyl sulfoxide, namely DMSO and fresh injection water into a solution with the volume concentration of 100%-50%; agitating uniformly and adding medical carbon; decarbonizing the obtained solution by a titanium rod and filtering the solution by a 0.45-micron micro-pore filter membrane; and filtering by a 0.22-micron micro-pore filter membrane to obtain the blood platelet preserving fluid. The preserving fluid can keep the physiological functions of blood platelets at a low temperature and has no toxic and side effects; and the damages of a receptor can be controlled in the lowest range.

The utility model discloses a platelet bag assembly capable of prolonging the storage life of platelets, which comprises a platelet storage bag, a first liquid medicine conduit and a plurality of platelet conduits, wherein the top of the platelet storage bag is provided with a PVC (Polyvinyl Chloride) material; platelet preservation liquid is stored in the liquid medicine bag, a second liquid medicine catheter made of PVC is arranged at the top of the liquid medicine bag, an easily-folded piece is arranged at the section, close to the liquid medicine bag, of the second liquid medicine catheter, and a Luer connector is arranged at the other end of the second liquid medicine catheter. Reliability of sterile connection is guaranteed by the aid of the catheter made of PVC (polyvinyl chloride), compatibility problem of long-term contact of materials and medicines is solved by the aid of the easy-to-fold part, sterility is realized by the aid of a novel Luer connector sealing structure, bag film materials of the platelet storage bag are optimized, retention period of platelets is prolonged from 5 days to 7 days, and the platelet storage bag is applicable to clinical application. The problem that the clinical platelet supply amount is insufficient is solved, and meanwhile the safety and convenience of the operation process are improved.

An object of the present invention is to provide a method for preserving a platelet in a refrigerated or frozen state while retaining more platelet functions, a platelet preservation solution for use in such a method, and the like. The present invention relates to a platelet preservation solution for refrigeration or freezing, comprising an electrolyte and an anticoagulant. The present invention also relates to a method for preserving a platelet, comprising: suspending a platelet in the platelet preservation solution according to the present invention; and preserving the suspension by refrigeration or freezing at 10° C. or lower.

The invention belongs to the technical field of platelet antibody detection, and in particular relates to an anti-sieve cell suitable for platelet antibody detection, its preparation method and application. The anti-screening cells were prepared by the following method: 1) 3 portions of O-type platelets that had been screened to confirm the antigen profile were mixed in equal volumes, washed with platelet cleaning solution, centrifuged, the supernatant was discarded, and the platelet precipitate was retained; 2) Resuspend the platelet pellet from step 1) in platelet loading solution and adjust the cell concentration to (1.0‑2.0)×10 10 cells / ml, shaking; 3) After shaking, use platelet cleaning solution to wash again. After cleaning, use platelet storage solution to resuspend the pellet, and adjust the cell concentration to (2.0‑5.0)×10 7 cells / ml to obtain anti-sieve cells suitable for platelet antibody detection. The platelet anti-sieve cells prepared by the method can be applied to platelet antibody detection, have a long storage time, are convenient to use, and have good antigen preservation effect.

The present invention relates to a new method for the preservation of blood platelets. In particular, the present invention provides a method which includes submitting the platelets to zeodration carried out at temperatures between 18° C. and 24° C. The dried platelets thus obtained can be preserved at room temperature for long period of time. The invention also provides kits and compositions for using the preserved platelets.

The invention relates to the technical field of platelet preservation, in particular to a clinical platelet cryopreservation technology. Through the synergistic effect of the low-toxicity composite cryopreservation liquid and the pulse type temperature control technology, the time limit that platelets are stored at normal temperature for 5 days traditionally is broken through, the storage period can be prolonged to half a year, and the cryopreservation liquid is suitable for platelet storage of autologous umbilical cord blood hematopoietic stem cell transplantation patients, meets the emergency blood use requirement and has good application prospects. In addition, the mixing speed of the blood platelets and the blood platelet cryoprotectant does not need to be strictly controlled during cryopreservation of the cryopreservation liquid, the blood platelets can be directly mixed, the cryopreservation method is simple, convenient and short in time consumption, meanwhile, the blood platelets cryopreserved through the method can be used without being washed after being recovered, the tedious and time-consuming washing step in a glycerin cryopreservation method is omitted, and the cost is reduced. The method has the remarkable advantages of simplicity and convenience in operation, short time consumption and high resuscitation efficiency.

The invention relates to a medical film material. The plastic body of the invention is PVC resin, which is added an appropriate of plasticizer and other additives, wherein, the weight content of all components is: 100 portion of PVC resin, 20 to 25 portion of dihexyl phthalate, 10 to 15 portion of di-n-octyl adipate, 1 to 5 portion of acetyl acid ester, 0 to 20 portion of epozy tetrahydro di-2-ethylhexyl phthalate, 2 to 10 portion of epoxidized soybean oil, 1 to 5 portion of EVA, 0.5 to 1 portion of stearate, 0.1 to 0.5 portion of lubricant polymethylphenyl siloxane fluid. The components are mixed, granulated, compressed and shaped, and made into the film material with thickness of 0.35 to 0.45mm. The invention has the advantages of reasonable prescription, good elasticity, soft and transparent film material, good permeability, good thermal stability and good low temperature resistance. On a high-frequency hot sealing machine, the film is hot sealed into bags in different sizes for storing blood and blood components; in particular the blood platelet storing bags can store the blood platelet for 5 days at normal temperature.

The invention provides blood platelet preserving fluid. A preparation method of the blood platelet preserving fluid comprises the following steps: preparing dimethyl sulfoxide, namely DMSO and fresh injection water into a solution with the volume concentration of 100%-50%; agitating uniformly and adding medical carbon; decarbonizing the obtained solution by a titanium rod and filtering the solution by a 0.45-micron micro-pore filter membrane; and filtering by a 0.22-micron micro-pore filter membrane to obtain the blood platelet preserving fluid. The preserving fluid can keep the physiological functions of blood platelets at a low temperature and has no toxic and side effects; and the damages of a receptor can be controlled in the lowest range.