Application of protein kinase a activator in platelet preservation and platelet preservation method

A protein kinase and activator technology, applied in the application field of protein kinase A activator in platelet preservation

Active Publication Date: 2021-04-13
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the storage temperature of platelets at room temperature is 22±2°C, but the storage time is about 3 days

Method used

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  • Application of protein kinase a activator in platelet preservation and platelet preservation method
  • Application of protein kinase a activator in platelet preservation and platelet preservation method
  • Application of protein kinase a activator in platelet preservation and platelet preservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] Under sterile conditions, the washed platelets were divided into three groups, untreated group, 5μM Forsklin group, and 0.5% DMSO group; the three groups of platelets were equally divided and stored at 0°C, 4°C, 10°C, 22°C, and 37°C For 5 days, 5 μL was taken every day, and 5 μL of washed platelets treated with A23187 at room temperature was used as a control.

[0039] figure 1 PS eversion was measured for flow cytometry in washed platelets treated with Forskolin and DMSO and stored for five days. Taking the DMSO group as the control, the PS valgus percentage is expressed as mean ± standard deviation. The above experiment was repeated 5 times, P<0.05; compared with the platelets in the DMSO group, the PS valgus percentage of the washed platelets in the Forskolin group decreased at the same time and in the storage environment. And the reduction became more obvious with the prolongation of time. On the 5th day, the difference in the effect of Forskolin on PS eversion of ...

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Abstract

The invention discloses the application of protein kinase A activator in platelet preservation and the platelet preservation method. It can be seen from the examples of the present invention that the present invention discloses for the first time the application of protein kinase A activators in inhibiting platelet apoptosis or maintaining platelet activity when the induction and regulation mechanism of platelet apoptosis is still unknown. Protein kinase A Activators can inhibit the loss of function caused by platelet apoptosis, and can protect platelets from apoptosis under storage and pathological stress conditions, thereby prolonging the survival time of platelets. It is a new strategy to improve the survival period of stored platelets. The results show that the present invention determines the lifespan and survival of platelets by regulating apoptosis, prolongs the storage time of platelets, improves the clinical application efficiency of platelets, and avoids the inactivation of stored platelets due to the loss of platelet function. And being scrapped has far-reaching significance.

Description

technical field [0001] The invention belongs to the technical field of platelet preservation, and in particular relates to the application of a protein kinase A activator in platelet preservation and a platelet preservation method. Background technique [0002] Platelets are the key factors regulating thrombus formation and pathological hemorrhage in the circulatory system, and also play an important role in the body's immune response, infection, atherosclerosis formation, and tumor metastasis and other pathophysiological processes. However, platelets have a short life cycle and can only survive for 8-9 days in peripheral blood. Fatal bleeding caused by thrombocytopenia is often seen in many common diseases such as infection, immune thrombocytopenia, diabetes, and some drug treatments, and the mechanism of shortened platelet lifespan caused by these diseases has not yet been fully understood. In addition, short-lived platelets, especially storage lesions, limit the shelf li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02A61K31/352A61P7/00
CPCA01N1/0226A61K31/352
Inventor 戴克胜
Owner SUZHOU UNIV
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