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Application of protein kinase A activator in blood platelet storage and blood platelet storage method

A protein kinase and preservation method technology, applied in the application field of protein kinase A activator in platelet preservation

Active Publication Date: 2017-08-18
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the storage temperature of platelets at room temperature is 22±2°C, but the storage time is about 3 days

Method used

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  • Application of protein kinase A activator in blood platelet storage and blood platelet storage method
  • Application of protein kinase A activator in blood platelet storage and blood platelet storage method
  • Application of protein kinase A activator in blood platelet storage and blood platelet storage method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0038] Under sterile conditions, the washed platelets were divided into three groups, untreated group, 5μM Forsklin group, and 0.5% DMSO group; the three groups of platelets were equally divided and stored at 0°C, 4°C, 10°C, 22°C, and 37°C For 5 days, 5 μL was taken every day, and 5 μL of washed platelets treated with A23187 at room temperature was used as a control.

[0039] figure 1 PS eversion was measured for flow cytometry in washed platelets treated with Forskolin and DMSO and stored for five days. Taking the DMSO group as the control, the PS valgus percentage is expressed as mean ± standard deviation. The above experiment was repeated 5 times, P<0.05; compared with the platelets in the DMSO group, the PS valgus percentage of the washed platelets in the Forskolin group decreased at the same time and in the storage environment. And the reduction became more obvious with the prolongation of time. On the 5th day, the difference in the effect of Forskolin on PS eversion of ...

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Abstract

The invention discloses an application of a protein kinase A activator in blood platelet storage and a blood platelet storage method. From the embodiment of the invention, the invention discloses an application of the protein kinase A activator in inhibiting blood platelet apoptosis or keeping the activity of blood platelets for the first time under circumstance that the inducing and adjusting mechanisms of blood platelet apoptosis are unknown. The protein kinase A activator can inhibit afunction induced by blood platelet apoptosis and can prevent the blood platelets from being affected by apoptosis in storage and pathological stress conditions, so that the blood platelet survival time is prolonged. The method is a novel policy of improving the survival period of the blood platelets. The result verifies that determination of service life and survival of the blood platelets by regulating apoptosis has far-reaching significance in prolonging the storage time of the blood platelets, increasing the clinical application efficiency of the blood platelets and preventing the stored blood platelets from being rejected due to afunction of the blood platelets.

Description

technical field [0001] The invention belongs to the technical field of platelet preservation, and in particular relates to the application of a protein kinase A activator in platelet preservation and a platelet preservation method. Background technique [0002] Platelets are the key factors regulating thrombus formation and pathological hemorrhage in the circulatory system, and also play an important role in the body's immune response, infection, atherosclerosis formation, and tumor metastasis and other pathophysiological processes. However, platelets have a short life cycle and can only survive for 8-9 days in peripheral blood. Fatal bleeding caused by thrombocytopenia is often seen in many common diseases such as infection, immune thrombocytopenia, diabetes, and some drug treatments, and the mechanism of shortened platelet lifespan caused by these diseases has not yet been fully understood. In addition, short-lived platelets, especially storage lesions, limit the shelf li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A61K31/352A61P7/00
CPCA01N1/0226A61K31/352
Inventor 戴克胜
Owner SUZHOU UNIV
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