The invention discloses a universal type fluorescent quantitative PCR method for detecting platelet bacterial pollution. The fluorescent quantitative PCR method employs a pair of universal type primers detecting bacteria, an artificial internal interference of the universal type primers, a first fluorescently-labeled fluorescent probe and a second fluorescently-labeled fluorescent probe; the two fluorescent probes employ different fluorescent labels; the first fluorescently-labeled fluorescent probe is a universal type fluorescent probe used for detecting bacteria, and the second fluorescently-labeled fluorescent probe is an internal reference probe used for detecting the artificial internal interference; and a routine PCR amplification technology is employed for detection. According to the above technical scheme, the detection method is suitable for screening on bacteria pollution of platelet products, is capable of fully solving the bacteria existence, bacteria content, bacteria survival state and other problems in bacteria pollution of the platelet products, satisfies rapid, accurate and reliable detection requirements, and provides a scientific effective auxiliary diagnosis method for safe infusion of platelet.