Novel platelet freeze-drying treating fluid

A technology for platelets and processing liquid, which is applied in the field of platelet freeze-drying and storage, and can solve the problems of product collapse, low mechanical strength, product separation from the wall, shrinkage, etc.

Active Publication Date: 2015-07-15
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the concentration of the buffer solution is too high, the surface of the liquid medicine will dry first when drying, forming a dense surface layer with small pores and poor air permeability, so it is difficult for the water vapor below to pass through the dry layer, so it rises along the bottle wall, causing the product to separate from the wall , shrivel; or make the water vapor stay in the dry layer for too long, and the dry surface product will absorb moisture again, causing the product to collapse and shrink in size; in addition,

Method used

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  • Novel platelet freeze-drying treating fluid

Examples

Experimental program
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Effect test

Embodiment 1

[0061] 1) Prepare the platelet freezing intervention treatment solution: add 50mmol / L trehalose and 1ug / mL PGE1 to the basic buffer solution, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.

[0062] 2) Prepare platelet freeze-drying buffer: add 50mmol / L trehalose, 1ug / mL PGE1 and 30% v / v plasma to the basic buffer, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.

[0063] 3) Intervention of platelet freezing:

[0064] 1 part of pooled platelets was centrifuged at 1500×g for 15 minutes, the supernatant was discarded and the precipitate was retained, the platelets were resuspended with the same volume of the discarded supernatant as the platelet freezing pretreatment solution, and the platelets were kept in a suspended state at 37°C for 4 hours in a shaking water bath. After the water bath, centrifuge the platelet suspension at 1500×g for 15 min, discard the supernatant and save the precipitate, and resuspend the platelets with the ...

Embodiment 2

[0069] 1) Prepare the platelet freezing intervention treatment solution: add 50mmol / L trehalose and 1ug / mL PGE1 to the basic buffer solution, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.

[0070] 2) Prepare platelet freeze-drying buffer: add 50mmol / L trehalose, 1ug / mL PGE1 and 40% v / v plasma to the basic buffer, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.

[0071] 3) Intervention of platelet freezing:

[0072] 1 part of pooled platelets was centrifuged at 1500×g for 15 minutes, the supernatant was discarded and the precipitate was retained, the platelets were resuspended with the same volume of the discarded supernatant as the platelet freezing pretreatment solution, and the platelets were kept in a suspended state at 37°C for 4 hours in a shaking water bath. After the water bath, centrifuge the platelet suspension at 1500×g for 15 min, discard the supernatant and save the precipitate, and resuspend the platelets with the ...

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Abstract

The invention discloses a novel platelet freeze-drying treating fluid. The fluid is composed of two parts: (1) a platelet freeze-drying pretreatment liquid, which is prepared by adding mycose and PGE1 into a basic buffer liquid; (2) a platelet freeze-drying buffer liquid, which is prepared by adding blood plasma into a freeze-drying pretreatment liquid. Preferably 30%v/v-40%v/v blood plasma is adopted to replace human serum albumin, a good effect is achieved, and the blood plasma is taken as the platelet protein protective agent to replace the pricy albumin and is economic. The osmotic pressure of the pretreatment liquid is 311.6 mOsm, is similar to the blood plasma osmotic pressure, and thus will not generate adverse effect on platelet. The weight concentration of the freeze-drying buffer liquid is 29%w/v, and the obtained freeze-dried platelet has a qualified appearance. The freeze-dried platelet can be stably preserved at a room temperature for at least 6 months, and the platelet can be used to prepare hemostatic or platelet products used in operation.

Description

technical field [0001] The invention relates to the field of platelet freeze-drying and preservation, in particular to a platelet freeze-drying treatment solution. Background technique [0002] Freeze-drying of platelets began in the 1950s, but the results were not as successful as expected. Until 2001, Wolkers WF et al. conducted another study on the freeze-drying of platelets. For the first time, trehalose was used to pretreat the platelets before freeze-drying, and the platelets were successfully freeze-dried. Predecessors' attempts to freeze-dry platelet storage indicate the possibility of platelet freeze-dry storage. Freeze-drying platelet storage has become a research hotspot. Many domestic units have also conducted freeze-drying research on platelets, but the freeze-drying formula reported so far There are some deficiencies, such as human serum albumin is used as the protein protective agent in the formula, which is expensive and not easy to obtain; it is also pretre...

Claims

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Application Information

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IPC IPC(8): A61K35/19A61K47/26A61K47/42A61K47/46A61K47/12A61K47/18A61P7/04
Inventor 单桂秋马静耿文艳张雅妮吕品程飚
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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