Chromatographic test strip for detecting platelet product bacterial pollution and detection method

A chromatographic test paper and bacterial contamination technology, applied in the field of microbiological detection, can solve the problems of no bacterial contamination of blood and blood components, poor physical condition, cumbersome operation, etc., and achieve the goal of saving platelet resources, reducing economic burden, and improving quality of life Effect

Inactive Publication Date: 2015-04-08
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) Clinically, platelet transfusion patients are mostly critically ill patients with poor basic physical conditions and low immunity, unable to effectively remove the imported bacteria, prone to bacteremia, sepsis and sepsis, and even death in severe cases
The current detection method has three main problems: time-consuming, cumbersome operation, low sensitivity, and cannot well meet the

Method used

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  • Chromatographic test strip for detecting platelet product bacterial pollution and detection method

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Experimental program
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Effect test

Embodiment 1

[0023] see figure 1 , providing a chromatographic test strip for detecting bacterial contamination of platelet products, including a sample pad 1, two reaction membranes 2, two absorbent pads 3 and a bottom plate 4. The two reaction membranes 2 are respectively located on the bottom plate 4 . The sample pad 1 is located in the middle of the bottom plate 4 , and the two ends of the sample pad 1 overlap and partially overlap one end of the reaction membrane 2 . The two absorbent pads 3 are located on the left and right ends of the bottom plate 4 , and one end of the absorbent pads 3 overlaps and partially overlaps the other end of the reaction membrane 2 that is not overlapped with the sample pad 1 . A detection zone 5 and an internal quality control zone 6 are fixed on the reaction membrane 2, generally an internal quality control zone 6 and at least one detection zone 5 are fixed on each reaction membrane 2, and the detection zone 5 is located near the One side of the sample...

Embodiment 2

[0025] A method for detecting bacterial contamination of platelet products with a chromatographic test strip is provided, the method is a visual identification result:

[0026] 1. Preparation of test strips

[0027] (1) Preparation of reaction membrane

[0028] Select Whatman Immunopore RP as the reaction membrane, cut it into a size of 30×1.8cm, and keep two sheets for spare. Adjust the concentration of anti-mouse IgG to 0.25mg / ml with 0.015M phosphate buffer solution with a pH of 7.2, add Tween-20 with a volume fraction of 1%, and spray it on the membrane surface as a quality control line , the amount of scratch film was 1 μl / cm. Use 0.015M pH 7.2 phosphate buffer solution to adjust the concentration of the monoclonal antibodies specific for lipopolysaccharide and phosphatidic acid to 0.25mg / ml, add Tween-20 with a volume fraction of 1%, and cut the membrane with The instrument sprays it on the surface of the two membranes as a detection line. The interval between each l...

Embodiment 3

[0042] A method for detecting bacterial contamination of platelet products with a chromatographic test strip is provided, the method is based on the results of fluorescence identification:

[0043] 1. Preparation of test strips

[0044] (1) Preparation of reaction membrane

[0045] Choose Whatman PRIMA60 as the reaction film, cut it into a size of 30×1.8cm, and keep two sheets for spare. Adjust the concentration of anti-mouse IgG to 0.25mg / ml with 0.015M phosphate buffer solution with a pH of 7.2, add Tween-20 with a volume fraction of 1%, and spray it on the membrane surface as a quality control line , the amount of scratch film was 1 μl / cm. The monoclonal antibodies specific for lipopolysaccharide and phosphatidic acid were adjusted to 0.25mg / ml with 0.015M phosphate buffer with a pH of 7.2, and Tween-20 with a volume fraction of 1% was added. The film meter sprays it on the surface of the two films as a detection line. The interval between each line is 5mm. Immediately...

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Abstract

The invention discloses a chromatographic test strip for detecting platelet product bacterial pollution and a detection method. The test strip consists of a sample pad, reaction films and an absorption pad which are mutually lapped on a bottom plate, wherein two ends of the sample pad are respectively lapped with two reaction films; each reaction film is fixedly provided with an inner quality control band and at least one detection band; the two reaction films are capable of respectively detecting the gram positive bacterium and negative bacterium in the platelet pollution; the sample in the sample pad can simultaneously diffuse toward the reaction films at two sides. Through the manner, the chromatographic test strip is capable of solving the technical problem that the traditional detection technology of the platelet product bacterial pollution is long in time, tedious to operate and low in sensitivity, so that the detection of conventional platelet product bacterial pollution before blood transfusion can be carried out clinically so as to avoid the blood transfusion diseases caused by bacterial pollution, improve the life quality of the patients and guarantee the clinically safe, effective and scientific blood transfusion.

Description

technical field [0001] The invention relates to the field of microbial detection, in particular to a chromatographic test strip and a detection method for detecting bacterial contamination of platelet products. Background technique [0002] Blood products are an important component of clinical disease treatment and first aid, which are directly related to the life safety of patients. However, the problem of bacterial contamination of blood products, especially platelets, is becoming more and more serious in clinical practice. Studies have shown that about 1 unit of platelet products is contaminated with bacteria in every 3000 units. The main source is the skin flora of blood donors, asymptomatic bacteremia in blood donors and contamination during the preparation process. If platelet products with bacterial contamination are transfused into patients, almost without exception, they will cause transfusion reactions of varying degrees, and even fatal bacteremia, sepsis and seps...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/577
CPCG01N33/56911G01N33/558
Inventor 田晶晶李勇段生宝王红梅陈晔洲丁少华蒙青林魏双施
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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