The invention discloses multi-parameter immunochromatographic assay test paper and a preparation method thereof. The multi-parameter immunochromatographic assay test paper comprises an immunochromatographic assay test paper lining plate, and is characterized in that a plurality of solid phase membranes, which are separated from each other, corresponding conjugate pad membranes, a sample pad membrane and an absorbent pad membrane are fixed on the immunochromatographic assay test paper lining plate, a test line reagent and a control line reagent are coated on the solid phase membranes which are separated from each other, and a colloidal particle reagent corresponding to needed detection indexes is arranged on the conjugate pad membranes. The immunochromatographic assay test paper for parallel tests by multiple parameters provided by the invention can be applied to the rapid and integrated detection analysis needed in clinic, food, medicines, chemical industry, environment, stock farming, agriculture and other aspects, and has the advantages that the limitation on production efficiency caused by existing single-parameter and multi-parameter immunochromatographic assay test paper preparation processes can be overcome, that the all performance of the test paper is not inferior to that of detection with a single detection index, that the separated detection of the multiple test indexes can be avoided when screening is implemented in physical examination, blood donation, blood transfusion and the like, that the operation is convenient and rapid, that the detection time is shortened, that the efficiency is improved, that the cost is lowered, that manpower and material resources are saved, and that the industrial production is easier.