Multi-parameter immunochromatographic assay test paper and preparation method thereof

Abstract

The invention discloses multi-parameter immunochromatographic assay test paper and a preparation method thereof. The multi-parameter immunochromatographic assay test paper comprises an immunochromatographic assay test paper lining plate, and is characterized in that a plurality of solid phase membranes, which are separated from each other, corresponding conjugate pad membranes, a sample pad membrane and an absorbent pad membrane are fixed on the immunochromatographic assay test paper lining plate, a test line reagent and a control line reagent are coated on the solid phase membranes which are separated from each other, and a colloidal particle reagent corresponding to needed detection indexes is arranged on the conjugate pad membranes. The immunochromatographic assay test paper for parallel tests by multiple parameters provided by the invention can be applied to the rapid and integrated detection analysis needed in clinic, food, medicines, chemical industry, environment, stock farming, agriculture and other aspects, and has the advantages that the limitation on production efficiency caused by existing single-parameter and multi-parameter immunochromatographic assay test paper preparation processes can be overcome, that the all performance of the test paper is not inferior to that of detection with a single detection index, that the separated detection of the multiple test indexes can be avoided when screening is implemented in physical examination, blood donation, blood transfusion and the like, that the operation is convenient and rapid, that the detection time is shortened, that the efficiency is improved, that the cost is lowered, that manpower and material resources are saved, and that the industrial production is easier.

Description

technical field [0001] The invention relates to a rapid detection technology that can be used for multi-parameter parallelization of indicators such as biomedicine, in particular to a multi-parameter immunochromatographic detection test paper and a preparation method thereof. Background technique [0002] Solid phase membrane-based immunoassay (solid phase membrane-based immunoassay) is similar to solid phase enzyme immunoassay (EL ISA), which is characterized by microporous membrane as the solid phase. Markers can be enzymes and various colored particles, such as colored latex, colloidal gold, colloidal selenium, etc., and red colloidal gold is the most commonly used. Solid phase membranes are characterized by their porosity, like filter paper. The solid phase membrane can be flowed through by the liquid, and the liquid can also move forward on the membrane by capillary action. Taking advantage of this property, two different types of rapid detection methods were establis...

AI Technical Summary

Problems solved by technology

The author believes that in the joint inspection of multi-parameter test strips, in order to ensure the specificity of the kit, the amount of colloidal gold cannot be increased; however, since several items are to be detected at the same time, the amount of colloidal gold corresponding to the target object should be reduced , which will cause the sensitivity of each item to be lower than that of individual detection, and in practical applications, there will be a problem of low sensitivity

At present, there is no immunochromatographic kit that can screen HBV, HCV, HIV, and TP at the same time. The main reason may be that it has not solved the problem that when several items are jointly detected, the sensitivity is not as good as individual detection.

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