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Method for detecting bacterial contamination of platelet product

A technology of bacterial contamination and platelets, which is applied in the field of medical testing, can solve the problem of low sensitivity of bacterial contamination and achieve the effects of reducing impact, improving sensitivity, and enhancing visibility

Pending Publication Date: 2019-11-15
苏州市中心血站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defect in the prior art that the sensitivity of bacterial contamination in the sample to be tested is not high through the aggregation and color development of carrier particles, thereby providing a method for detecting bacterial contamination of platelet products

Method used

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Embodiment 1

[0021] This scheme involves a detection method for bacterial contamination in platelet products, specifically taking Gram-positive bacteria or negative bacteria as an example, including the following steps:

[0022] S1. Preparation of the reaction membrane: select nitrocellulose membrane (NC membrane) as the reaction membrane, cut it into a size of 30×1.8 cm, and keep two for use. Adjust the concentration of anti-mouse IgG to 0.25mg / mL with 0.02M phosphate buffer solution with a pH of 7.2, add Tween-20 with a volume fraction of 1%, and spray it on the membrane surface as a quality control line , the amount of scratch film was 1 μL / cm. Use 0.02M pH 7.2 phosphate buffer solution to adjust the concentration of monoclonal antibodies specific to lipopolysaccharide and phospholipid teicidic acid to 0.2 mg / mL, add Tween-20 with a volume fraction of 1%, and draw the membrane using The instrument sprays it on the surface of the film as a detection line. The interval between each line...

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PUM

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Abstract

The invention relates to the technical field of medical examination, in particular to a method for detecting bacterial contamination of a platelet product. The method comprises the following steps: enriching and separating to-be-detected bacteria from a to-be-detected sample by using magnetic nanoparticles that have surfaces coupled with monoclonal or polyclonal antibodies or aptamers bound with the to-be-detected bacteria; resuspending the magnetic nanoparticles with the to-be-detected bacteria captured by using a buffer solution; inserting a piece of chromatography test paper into the buffersolution and carrying out reaction; taking out the chromatography test paper after reaction and immersing the chromatography test paper into a substrate capable of being catalyzed for color development by the magnetic nanoparticles to carry out secondary color development reaction; and taking out the paper, terminating the color development reaction, and observing results at a test strip and a quality control strip. With processing of the secondary color development reaction, the obviousness of the color change at the detection line can be enhanced, so that the detection sensitivity is improved and thus the detection result becomes accurate.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a detection method for bacterial contamination of platelet products. Background technique [0002] Blood products are an important component of clinical disease treatment and first aid, which are directly related to the life safety of patients. However, the problem of bacterial contamination of clinical blood products, especially platelets, is becoming more and more severe. Studies have shown that about 1 unit of platelet products in every 3000 units has bacterial contamination, and the main sources of contamination are skin flora of blood donors, asymptomatic bacteremia in blood donors, and contamination during the preparation process. However, clinically, most of the platelet transfusion patients are critically ill patients with poor basic physical conditions and low immunity, and cannot effectively remove the imported bacteria. Therefore, if the contaminated platelet ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56911G01N33/54306G01N33/54326G01N33/54346
Inventor 王明元严伟斌吴建香陈炜汤龙海
Owner 苏州市中心血站
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