Platelet antibody detection method based on nano material and platelet preservation method

An antibody detection and nanomaterial technology, applied in the field of blood transfusion medicine, achieves the effect of short technical time, low cost, and improved clinical infusion effect

Active Publication Date: 2020-10-23
SHANGHAI BLOOD CENT
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  • Platelet antibody detection method based on nano material and platelet preservation method
  • Platelet antibody detection method based on nano material and platelet preservation method

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Embodiment 1

[0040] The present invention mainly uses polylactic acid glycolic acid polymer (PLGA), and it is a kind of by lactic acid and glycolic acid synthesis, the functional organic macromolecule material of degradability, has good biocompatibility, physical and chemical properties, its strength , mechanical properties and degradation rate can be adjusted by the composition ratio and molecular weight of the copolymer, and has been certified by the FDA as a pharmaceutical excipient, so PLGA can be used as an ideal biomaterial and widely used in the biomedical field.

[0041] 1 Experimental materials and instruments

[0042]PLGA powder (purchased from sigma); machine-collected platelets were washed and resuspended; AB slurry (reacted with multiple random platelets, all antibodies were negative); BCA rapid detection kit (purchased from thermo); platelet preservation solution (ACD) As a washing solution; deionized water; anti-HPA1a antibody standard serum (NIBSC); acetone (purchased from ...

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Abstract

The invention relates to a platelet antibody detection method based on a nano material and a platelet preservation method. In the invention, nano composite particles prepared by wrapping PLGA with platelet membrane protein are applied to platelet antibody detection for the first time, which proves that the platelet antibody can be accurately detected by taking human-derived serum containing an anti-platelet antibody as a detection object, and good sensitivity is possessed. In the invention, improvement of clinical diagnosis of platelet immune abnormal diseases is facilitated, matched plateletsare provided for patients, a clinical infusion effect of the platelets is improved, clinical blood transfusion quality is increased, and precious blood resources are saved for the society at the sametime.

Description

technical field [0001] The invention relates to the technical field of blood transfusion medicine, in particular to a nanomaterial-based platelet antibody detection method and a platelet preservation method. Background technique [0002] Platelets are the smallest cellular component of human blood and their main function is to stop bleeding by initiating the coagulation cascade. Platelet count has been used as an important indicator for clinical assessment of bleeding risk, and its normal value is 100-300*10 9 / L. When the platelet count is lower than normal, it is called thrombocytopenia and is attributed to either a decrease in the number of platelets or an increase in platelet destruction. In recent years, with the development of blood transfusion medicine, component blood transfusion has been widely used, and platelet transfusion has become one of the important means of clinical blood transfusion therapy. However, with the massive use of platelets, ineffective platele...

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Application Information

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IPC IPC(8): G01N15/14G01N33/68G01N33/543G01N33/545A01N1/02
CPCG01N15/14G01N33/6854G01N33/54346G01N33/545A01N1/0278A01N1/0236
Inventor 马勤勤陆萍朱慧君李睿书傅敏
Owner SHANGHAI BLOOD CENT
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