The invention discloses a mixed polypeptide
analysis method, which uses a volume exclusion
chromatographic column in a
mixed mode, carries out separation by combining the hydrophobicity difference of samples on the basis of a common volume exclusion
chromatographic column, and can effectively solve the problem that the common volume exclusion
chromatographic column is difficult to effectively separate samples with small molecular
weight difference. Especially, different polypeptide components with similar molecular weights in the mixed polypeptide can be effectively separated, the steps are simple and easy to operate, and rapid and efficient analysis of the components of the mixed polypeptide can be realized without connecting other analysis methods in series.