Moderate molecular weighted chondroitin sulfate injection and its preparing process

A chondroitin sulfate and cartilage sulfate technology, which is applied in the directions of medical preparations containing active ingredients, bone diseases, and pharmaceutical formulations, to achieve the effects of high product quality, high preparation purity, and good pharmacological activity

Inactive Publication Date: 2005-11-02
汤毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no chondroitin sulfate product with a molecular weight lower than 30,000 Daltons on the market in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of medium molecular weight chondroitin sulfate

[0046] Put 4.5 liters of deionized water in a glass beaker, heat it to 70°C in a water bath, add 250 grams of commercially available chondroitin sulfate (average molecular weight 48,000 Daltons), stir and dissolve, add 145 ml of 36% hydrochloric acid to the solution, add deionized Water to 5 liters, so that the hydrochloric acidity of the solution is about 0.3N. After insulated and stirred for 6 hours, cool down rapidly, add 10% sodium hydroxide aqueous solution to make the pH of the solution around 10.5, and then use a centrifuge to remove the precipitate of miscellaneous proteins. The resulting supernatant was subjected to three-stage ultrafiltration with an ultrafiltration element with a molecular weight cut-off of 10,000 Daltons (polysulfone hollow fiber ultrafiltration membrane, Shanghai Yiming Filtration Technology Co., Ltd.), and then with a nanofiltration element (Hangzhou Water...

Embodiment 2

[0047] Embodiment 2 Preparation of medium molecular weight chondroitin sulfate

[0048] Put 4.5 liters of deionized water in a glass, heat it in a water bath to 80°C, add 300 grams of commercially available chondroitin sulfate (average molecular weight 48,000 Daltons) crude product, stir to dissolve, add 45 grams of oxalic acid to the solution, and add deionized water to 5 liters, so that the concentration of oxalic acid in the solution is about 0.1N. After heat preservation and stirring for 8 hours, cool down rapidly, add 50 grams of calcium carbonate to make the pH of the solution around 9.5, and then use a centrifuge to remove the precipitates of foreign proteins and calcium oxalate. The resulting supernatant is filtered with an ultrafiltration membrane with a molecular weight cut-off of 20,000 Daltons, and the nanofiltration membrane is concentrated. The concentrated solution is crystallized by adding 95% ethanol, and vacuum-dried at 60° C. to obtain 276.8 grams...

Embodiment 3

[0049] Embodiment 3 Preparation of medium molecular weight chondroitin sulfate

[0050] Put 650 liters of deionized water in a jacketed reaction tank, heat to 73°C, add 40 kg of commercially available crude product of common molecular weight chondroitin sulfate (average molecular weight 48,000 Daltons), stir and dissolve, add 11.2 liters of 36% hydrochloric acid to the solution, The hydrochloric acid concentration of the solution is about 0.2N. After insulated and stirred for 4 hours, cool down rapidly, add 7.0 kg of sodium carbonate to make the pH of the solution around 10.5, and then use a continuous flow centrifuge to remove the precipitate of miscellaneous proteins. The resulting supernatant is filtered with an ultrafiltration membrane with a molecular weight cut-off of 20,000 Daltons, the nanofiltration membrane is concentrated, and the concentrated solution is spray-dried to obtain 37 kilograms of white medium molecular weight chondroitin sulfate (average molecu...

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Abstract

The present invention discloses the injection of chondroitin sulfate in medium molecular weight and its preparation process. The injection of chondroitin sulfate includes chondroitin sulfate in medium molecular weight of 10,000-30,000 daltons and / or its salt and medicinal additive, and the medium molecular weight chondroitin sulfate is prepared through ultrafiltering and nanofiltering. Compared with common injection of chondroitin sulfate, the injection of the present invention has smaller molecular weight, higher pharmacological activity and higher purity, and can meet the requirement for safe administration.

Description

technical field [0001] The invention relates to a medium molecular weight chondroitin sulfate injection and a preparation method thereof. Specifically, the invention relates to a medium molecular weight chondroitin sulfate injection with a molecular weight of 10,000-30,000 Daltons and a preparation method thereof. Background technique [0002] Chondroitin Sulfate is a macromolecular acidic mucopolysaccharide prepared from the cartilage tissue of pigs, cattle, sheep and other animals and the cartilage of aquatic products such as sharks by using biochemical extraction technology. Chondroitin sulfate sodium salt, potassium salt, calcium salt, zinc salt, etc. are widely used in medicine, health products, food, cosmetics, pet feed, etc. For example, chondroitin sulfate can be used as a blood lipid-lowering drug for clinical treatment of hyperlipidemia. Chondroitin Sulfate A Sodium Injection has the effects of lowering blood lipids, resisting atherosclerotic plaque formation, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/737
Inventor 汤毅
Owner 汤毅
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